A5036313663- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine Sponges and Natural Products
- Marine and coastal ecosystems
- Microbial Metabolism and Applications
- Algal biology and biofuel production
- Essential Oils and Antimicrobial Activity
- Biocrusts and Microbial Ecology
- Protist diversity and phylogeny
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Diatoms and Algae Research
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Botanical Research and Applications
- Botany and Geology in Latin America and Caribbean
- Mediterranean and Iberian flora and fauna
- Botany, Ecology, and Taxonomy Studies
- Environmental Toxicology and Ecotoxicology
- Proteoglycans and glycosaminoglycans research
- Plant Diversity and Evolution
- Microbial bioremediation and biosurfactants
Universidade do Porto
2016-2025
University of Helsinki
2020-2021
Centro Interdisciplinar de Investigação Marinha e Ambiental
2018-2020
Universidade Castelo Branco
2020
Abstract With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role the discovery novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these are often organised into gene clusters (BGCs). In 2015, we defined Minimum Information about Biosynthetic Gene cluster (MIBiG): standardised format describes minimally required information to...
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Abstract Specialized or secondary metabolites are small molecules of biological origin, often showing potent activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis these natural products is governed by sets co-regulated physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs a standardized machine-readable way, Minimum Information Biosynthetic Gene cluster (MIBiG) data standard repository was initiated...
Cyanobacteria are a well-known source of bioproducts which renders culturable strains valuable resource for biotechnology purposes. We describe here the establishment cyanobacterial culture collection (CC) and present first version strain catalog its online database (http://lege.ciimar.up.pt/). The LEGE CC holds 386 strains, mainly collected in coastal (48%), estuarine (11%), fresh (34%) water bodies, most part from Portugal (84%). By following recent taxonomic classification, were...
Bacterial biofilms are complex biological systems that difficult to eradicate at a medical, industrial, or environmental level. Biofilms confer bacteria protection against external factors and antimicrobial treatments. Taking into account about 80% of human infections caused by bacterial biofilms, the eradication these structures is great priority. resistant old-generation antibiotics, which has led search for new antimicrobials from different sources, including deep oceans/seas. In this...
Lipids are one of the primary metabolites microalgae and cyanobacteria, which enrich their utility in pharmaceutical, feed, cosmetic, chemistry sectors. This work describes isolation, structural elucidation, antibiotic antibiofilm activities diverse lipids produced by different cyanobacteria strains from two European collections (ACOI LEGE-CC). Three strain were selected for antibacterial and/or activity after screening about 600 carried out under NoMorFilm project. The total organic...
Essential trace metals are vital for cellular processes such as respiration, DNA replication, and photosynthesis. Cyanobacteria must tightly regulate metal homeostasis to prevent deficiency or toxicity, yet their metallophores remain overlooked. Here, we report lusichelins A-E (1-5), new isolated from the marine cyanobacterium Lusitaniella coriacea LEGE 07167. Their structures configurational assignments were determined using NMR, mass spectrometry, TD-DFT calculations, retrobiosynthetic...
Recent analyses of genome data indicate that members the cyanobacterial order Pleurocapsales show tremendous potential for natural product discovery. However, only a few compounds have been reported from this order. Here, we report isolation hyellamide (1), glycosylated N-acyl tyrosine-derived eneamide, pleurocapsalean cyanobacterium Hyella patelloides LEGE 07179. The putative biosynthetic gene cluster 1 was identified in producing organism and proposal is presented. This work sheds light on...
Microbial mats are complex, micro-scale ecosystems that can be found in a wide range of environments. In the top layer photosynthetic from hypersaline environments, large diversity cyanobacteria typically predominates. With aim strengthening knowledge on cyanobacterial present coastal lagoon system Araruama (state Rio de Janeiro, Brazil), we have characterized three mat samples by means polyphasic approach. We used morphological and molecular data obtained culture-dependent -independent...
Blooms of cyanobacteria have negative effects on aquatic organisms and even human health, since many species can synthesize dangerous toxins. Studies their potential toxicity been very scarce in Chile limited to lakes the central zone. Recently, North‐Patagonian lakes, although characterized by low nutrient concentration productivity, revealed also presence blooms cyanobacteria, mainly constituted Microcystis Dolichospermum (former Anabaena sp.). To identify toxicological risk associated...
Human poisoning by microcystin has been recorded in many countries, including Brazil, where fatal cases have already occurred. The Amazon River is the main source of drinking water municipalities such as Macapá, there no monitoring cyanobacteria and cyanotoxins. This study investigated presence cyanotoxins samples from a treatment plant (DWTP) that catches River. toxin analyses employed ELISA, LC/MS, molecular screening for genes involved production sampling was carried out monthly April...
Microginins are a large family of cyanobacterial lipopeptide protease inhibitors. A hybrid polyketide synthase/non-ribosomal peptide synthetase biosynthetic gene cluster (BGC) found in several microginin-producing strains─mic─was proposed to encode the production microginins, based on bioinformatic analysis. Here, we explored cyanobacterium, Microcystis aeruginosa LEGE 91341, which contains mic BGC, discover 12 new microginin variants. The compounds contain uncommon amino acids, namely,...
Microbial natural products are compounds with unique chemical structures and diverse biological activities. Cyanobacteria commonly possess a wide range of biosynthetic gene clusters (BGCs) to produce products. Although product BGCs have been found in almost all cyanobacterial genomes, little attention has given research the partitioning these pathways chromosomes plasmids. Cyanobacterial plasmids believed disperse several BGCs, such as toxins, by through horizontal transfer. Therefore, may...
Small, single-celled planktonic cyanobacteria are ubiquitous in the world's oceans yet tend not to be perceived as secondary metabolite-rich organisms. Here we report isolation and structure elucidation of hierridin C, a minor metabolite obtained from cultured picocyanobacterium Cyanobium sp. LEGE 06113. We describe simple, straightforward synthetic route scarcely produced hierridins that relies on key regioselective halogenation step. In addition, show these compounds originate type III PKS...
Four natural lactylates of chlorinated fatty acids, chlorosphaerolactylates A–D (1–4), were isolated from the methanolic extract cyanobacterium Sphaerospermopsis sp. LEGE 00249 through a combination bioassay-guided and MS-guided approaches. Compounds 1–4 are esters (mono-, di-, or tri)chlorinated lauric acid lactic acid, whose structures assigned on basis spectrometric spectroscopic methods inclusive 1D 2D NMR experiments. High-resolution mass-spectrometry data sets also demonstrated...
Lactylates are an important group of molecules in the food and cosmetic industries. A series natural halogenated 1-lactylates, chlorosphaerolactylates (1–4), were recently reported from Sphaerospermopsis sp. LEGE 00249. Here, we identify cly biosynthetic gene cluster, containing all necessary functionalities for biosynthesis lactylates, based on silico analyses. Using a combination stable isotope incorporation experiments bioinformatic analysis, propose that dodecanoic acid pyruvate key...
The Amazon region contains a great diversity of species, and the River basin accounts for almost 20% all freshwater in world. Despite favorable environmental conditions this region, little is known about cyanobacterial waterbody, especially at mouth river. In paper, we used polyphasic approach to identify 14 strains isolated on inlet site from drinking water supply located close river mouth. were characterized based morphology, behavior culture, 16S rRNA gene sequencing, phylogenetic...
A new species Temnogametum iztacalense sp. nov. (Zygnemataceae) was described using an integrative taxonomic approach. Material collected between 2014–2019 from purple mats at the bottom of La Luna Lake, which is a shallow, well-oxygenated, acidic and ultraoligotrophic high mountain lake exposed to levels ultraviolet radiation. Morphological analysis revealed that material belonged Zygnemataceae contained mature zygospores oblate-sphaeroidal shape; smooth yellow exospores, finely punctate...
Abstract Background Halogenation is a recurring feature in natural products, especially those from marine organisms. The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. Recently, CylC – the first predicted dimetal-carboxylate halogenase to be characterized was shown regio- and stereoselectively install chlorine atom onto an unactivated carbon center during cylindrocyclophane biosynthesis. Homologs of are...
Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin β-N-methylamino-L-alanine-BMAA) using molecular chemical methods. All isolates free of target genes involved toxin biosynthesis. Only DNA from amplified mcyE gene. Presence microcystin-LR BMAA extracts was discarded by LC/MS analyses. The analysed have an incomplete microcystin biosynthetic...
The McMurdo Dry Valleys constitute the largest ice-free region of Antarctica and one most extreme deserts on Earth. Despite low temperatures, dry poor soils katabatic winds, some microbes are able to take advantage endolithic microenvironments, inhabiting pore spaces soil constituting photosynthesis-based communities. We isolated a green microalga, Endolithella mcmurdoensis gen. et sp. nov, from an sandstone sample collected in (Victoria Land, East Antarctica) during K020 expedition, January...