A5032263286- Microbial Natural Products and Biosynthesis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Synthetic Organic Chemistry Methods
- Marine Sponges and Natural Products
- Chemical Synthesis and Analysis
- Chemical synthesis and alkaloids
- Plant biochemistry and biosynthesis
- Alkaloids: synthesis and pharmacology
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Molecular spectroscopy and chirality
- Enzyme Catalysis and Immobilization
- Axial and Atropisomeric Chirality Synthesis
- Fungal Biology and Applications
- Ubiquitin and proteasome pathways
- Catalytic C–H Functionalization Methods
- Photosynthetic Processes and Mechanisms
- Natural product bioactivities and synthesis
- Peptidase Inhibition and Analysis
- Gut microbiota and health
- Synthesis and Biological Activity
- Biochemical and Structural Characterization
- Radical Photochemical Reactions
- Fluorine in Organic Chemistry
Helmholtz Centre for Infection Research
2023-2025
Saarland University
2023-2025
TU Dresden
2018-2025
Helmholtz Institute for Pharmaceutical Research Saarland
2023-2025
Center for Integrated Protein Science Munich
2014-2022
Technical University of Munich
2013-2022
Scripps Institution of Oceanography
2009-2022
University of California, San Diego
2011-2022
Max Planck Computing and Data Facility
2015-2021
University of Bonn
2010-2014
Our knowledge about the gut microbiota of pigs is still scarce, despite importance these animals for biomedical research and agriculture. Here, we present a collection cultured bacteria from pig gut, including 110 species across 40 families nine phyla. We provide taxonomic descriptions 22 novel 16 genera. Meta-analysis 16S rRNA amplicon sequence data metagenome-assembled genomes reveal prevalent pig-specific within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus,...
Bacteria are a major source of natural products that provide rich opportunities for both chemical and biological investigation. Although the vast majority known bacterial metabolites derive from free-living organisms, increasing evidence supports widespread existence chemically prolific bacteria living in symbioses. A strategy based on bioinformatic prediction, symbiont cultivation, isotopic enrichment, advanced analytics was used to characterize unique polyketide, nosperin,...
Abstract Polycyclic tetramate macrolactams (PTMs) are a family of biomedically promising natural products with challenging molecular frameworks. Despite these interesting properties, so far only relatively little is known about the biosynthetic origin PTMs, in particular concerning mechanism by which their ring systems formed. Herein we present first insights into processes using biosynthesis ikarugamycin as an example. This has been facilitated heterologous expression PTM gene cluster...
Reported is the structure and biosynthesis of ansalactam A, an ansamycin class polyketide produced by unusual modification pathway. This new metabolite, a marine sediment-derived bacterium genus Streptomyces, possesses novel spiro γ-lactam moiety distinctive isobutyryl fragment observed for first time in this natural products. The A was defined spectroscopic methods including X-ray crystallographic analysis. Biosynthetic studies with stable isotopes further led to discovery new, branched...
Axially chiral biaryl compounds are frequently encountered in nature where they exhibit diverse biological properties. Many biphenols that have C–C or C–O linkages installed by cytochrome P450 oxygenases control the regio- and stereoselectivity of intermolecular coupling reaction. In contrast, bipyrrole-coupling enzymology has not been observed. Marinopyrroles, produced a marine-derived streptomycete, first 1,3′-bipyrrole natural products. On basis marinopyrrole's unusual bipyrrole...
The need for new pharmacological lead structures, especially against drug resistances, has led to a surge in natural product research and discovery. New biosynthetic gene cluster capturing methods efficiently clone heterologously express pathways have thus been developed. Direct pathway cloning (DiPaC) is an emerging synthetic biology strategy that utilizes long-amplification PCR HiFi DNA assembly the capture expression of clusters. Here, we further streamlined DiPaC by reducing time reagent...
Abstract Natural products are a virtually inexhaustible source of small molecules with spectacular molecular architectures and biomedical potential. Their structural complexity generates formidable challenges to total synthesis but often also precludes time‐ resource‐efficient, stereoselective synthetic access. Biosynthetically, nature frequently uses dimerization oligomerization reactions produce highly challenging frameworks from simple starting materials. Impressive examples the...
Abstract The sorbicillinoid family is a large class of natural products known for their structural variety and strong, diverse biological activities. A special member this family, sorbicillactone A, the first nitrogen-containing sorbicillinoid, exhibits potent anti-leukemic anti-HIV activities possesses unique structure formed from sorbicillinol, alanine, fumaric acid building blocks. To facilitate in-depth structure-activity relationship studies promising product, we developed...
In this paper, the synthesis of a new type intrinsically chiral, directly β,β′-linked, octa-meso-aryl-substituted bisporphyrins is described, by using an optimized Suzuki−Miyaura coupling procedure. This strategy leads to broad variety such axially chiral 'superbiaryls', differing in their metalation states and substitution patterns. On basis efficient route as-yet-unknown β-boronic acid esters various meso-tetraarylporphyrins (TAPs) two-step bromination−borylation protocol, 18 bisporphyrin...
A marine bacterium, strain Pol012(T), was isolated from the Mediterranean sponge Axinella polypoides and subsequently characterized as belonging to subphylum 1 of phylum 'Verrucomicrobia'. Strain Pol012(T) non-motile, Gram-negative, coccoid or rod-shaped red in colour. The menaquinones MK-8 MK-9 were detected. G+C content genomic DNA 50.9 mol%. Growth possible at temperatures between 8 30 degrees C pH values 6.8 8.2. closest cultured relative Akkermansia muciniphila (83 % sequence...
Abstract Nature provides an inexhaustible diversity of small organic molecules with beautiful molecular architectures that have strong and selective inhibitory activities. However, this tremendous biomedical potential often remains inaccessible, as the structural complexity natural products can render their synthetic preparation extremely challenging. This problem is addressable by harnessing biocatalytic procedures evolved nature. In work, we present enzymatic total synthesis ikarugamycin....
Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to identification a putative sodorifen biosynthetic gene cluster (BGC). The is volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (C16H26) produced by strains S. plymuthica. BGC encoding consists four genes, two which (sodA, sodB) are homologs genes enzymes...
Abstract Background The biosynthesis of the natural product family polycyclic tetramate macrolactams (PoTeMs) employs an uncommon iterative polyketide synthase/non-ribosomal peptide synthetase (iPKS/NRPS). This machinery produces a universal PoTeM biosynthetic precursor that contains tetramic acid moiety connected to two unsaturated polyene side chains. enormous structural and hence functional diversity PoTeMs is enabled by pathway-specific tailoring enzymes, particularly...
ABSTRACT Ikarugamycin is a member of the natural product family polycyclic tetramate macrolactams (PoTeMs). The compound exhibits diverse range biological activities, including antimicrobial, antiprotozoal, anti‐leukemic, and anti‐inflammatory properties. In addition, it interferes with several crucial cellular functions, such as oxidized low‐density lipoprotein uptake in macrophages, Nef‐induced CD4 cell surface downregulation, mechanisms endocytosis. It is, therefore, used tool to study...
Human health is greatly influenced by the gut microbiota and imbalance can lead to development of diseases. It widely acknowledged that interaction bacteria within competitive ecosystems their specialized metabolites, which act, e.g., as antibacterials or siderophores. However, our understanding occurrence impact such natural products in human microbiome remains very limited. As arylthiazole siderophores are an emerging family growth-promoting molecules pathogenic bacteria, we analyzed a...
Cyanobacteria have long been known as a prolific source of bioactive natural products (NPs). One such group NPs are the furanolides, whose first congeners were discovered in 1980s and 1990s. A structural characteristic furanolides is γ-butyrolactone moiety with aromatic or aliphatic substituents at α- β-positions an substituent its γ-position. Recent elucidation genetic basis furanolide formation enables genome mining approaches to discover related cryptic biosynthetic gene clusters (BGCs)....