A5061581521- Algal biology and biofuel production
- Reproductive biology and impacts on aquatic species
- Reproductive Biology and Fertility
- Marine Sponges and Natural Products
- Sperm and Testicular Function
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Advanced Nanomaterials in Catalysis
- Environmental Toxicology and Ecotoxicology
- Diatoms and Algae Research
- Fish Biology and Ecology Studies
- Nanoparticles: synthesis and applications
- Metabolomics and Mass Spectrometry Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- bioluminescence and chemiluminescence research
- Microbial Community Ecology and Physiology
- Bioactive Natural Diterpenoids Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Autophagy in Disease and Therapy
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Environmental Chemistry and Analysis
- Microbial Fuel Cells and Bioremediation
- Advanced Proteomics Techniques and Applications
Universidade do Porto
2013-2025
Centro Interdisciplinar de Investigação Marinha e Ambiental
2017-2023
University of Aveiro
2015-2018
Institute of Biomedical Science
2013
Titanium dioxide (TiO2) and silver (Ag) NPs are among the most used engineered inorganic nanoparticles (NPs); however, their potential effects to marine demersal fish species, not fully understood. Therefore, this study aimed assess proteomic alterations induced by sub-lethal concentrations citrate-coated 25 nm ("P25") TiO2 or polyvinylpyrrolidone (PVP) coated 15 Ag turbot, Scophthalmus maximus. Juvenile were exposed through daily feeding for 14 days. The tested 0, 0.75 1.5 mg of each per kg...
Significance The dinoflagellate genus Symbiodinium is best known for harboring important endosymbiotic algae of marine invertebrates, notably reef-building corals. However, these dinoflagellates also live freely within coral reef waters and sediments provide an environmental pool the colonization new recruits. Although facilitate calcification indirectly when in hospite , we show that they can calcify partnership with bacteria free living. This discovery offers entirely perspectives on...
Marine organisms, particularly cyanobacteria, are important resources for the production of bioactive secondary metabolites treatment human diseases. In this study, a bioassay-guided approach was used to discover with lipid-reducing activity. Two chlorophyll derivatives were successfully isolated, previously described 132-hydroxy-pheophytin (1) and new compound 132-hydroxy-pheofarnesin (2). The structure elucidation 2 established based on one- two-dimensional (1D 2D) NMR spectroscopy mass...
Marine organisms, including seagrasses, are important sources of biologically active molecules for the treatment human diseases. In this study, organic extracts marine seagrass Halophila stipulacea obtained by different polarities from leaves (L) and stems (S) (hexane [HL, HS], ethyl acetate [EL, ES], methanol [ML, MS]) were tested bioactivities. The screening comprehended cytotoxicity activity against cancer cell lines grown as a monolayer culture or multicellular spheroids (cancer),...
The extensive use of nanomaterials, including titanium dioxide nanoparticles (TiO2 NPs), raises concerns about their persistence in ecosystems. Protecting aquatic ecosystems and ensuring healthy safe aquaculture products requires the assessment potential impacts NPs on organisms. Here, we study effects a sublethal concentration citrate-coated TiO2 two different primary sizes over time flatfish turbot, Scophthalmus maximus (Linnaeus, 1758). Bioaccumulation, histology gene expression were...
Essential trace metals are vital for cellular processes such as respiration, DNA replication, and photosynthesis. Cyanobacteria must tightly regulate metal homeostasis to prevent deficiency or toxicity, yet their metallophores remain overlooked. Here, we report lusichelins A-E (1-5), new isolated from the marine cyanobacterium Lusitaniella coriacea LEGE 07167. Their structures configurational assignments were determined using NMR, mass spectrometry, TD-DFT calculations, retrobiosynthetic...
Abstract Cyanobacteria, known to be rich sources of valuable natural products (NPs) with relevant biological properties, are a unique subject study the interplay between chemistry and ecology. Cultivation cyanobacteria as isolated strains may only reveal small fraction their NPs. In contrast, investigating microbial interactions from an ecological perspective is particularly fruitful approach unveil both new bioactivity. Cyanobacteria amoebae co-exist in diverse environments, but interaction...
Pursuing effective and biocompatible natural compounds to supplant current biocidal antifouling (AF) technologies remains crucial challenging. Among products hosts, cyanobacteria are recognized as producers of bioactive secondary metabolites that underexplored in terms anti-biofilm AF potential. Nocuolin A, a oxadiazine previously isolated known be produced by different cyanobacterial strains, has demonstrated potential, particularly against tumor cell lines. Considering this potential its...
Portoamides are cyclic peptides produced and released by the cyanobacterial strain Phormidium sp. presumably to interfere with other organisms in their ecosystems ("allelopathy"). were previously demonstrated have an antiproliferative effect on human lung carcinoma cells, but underlying mechanism of this activity has not been described. In present work, effects portoamides proliferation examined eight cancer cell lines two non-carcinogenic lines, major differences sensitivities observed. To...
Natural products are interesting sources for drug discovery. The natural product oxadiazine Nocuolin A (NocA) was previously isolated from the cyanobacterial strain Nodularia sp. LEGE 06071 and here we examined its cytotoxic effects against different strains of colon cancer cell line HCT116 immortalized epithelial hTERT RPE-1. NocA cells under conditions exponential growth but only weakly active non-proliferating cells. induced apoptosis by mechanism(s) resistant to overexpression BCL family...
Abstract Esterification reactions are central to many aspects of industrial and biological chemistry. The formation carboxyesters typically occurs through nucleophilic attack an alcohol onto the carboxylate carbon. Under certain conditions employed in organic synthesis, nucleophile can be alkylated generate esters from alkyl halides, but this reaction has only been observed transiently enzymatic Here, we report a alkylating enzyme – BrtB that catalyzes O-C bond between free fatty acids...
A Gram-stain-negative, facultatively anaerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain SYM1 T , was isolated from a culture of Symbiodinium sp., an algal symbiont the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth observed at 4–40 °C (optimum 30 °C), pH 5.0–11.0 8.0) with 0.5–8 % 2 %) (w/v) NaCl. Phylogenetic analyses 16S rRNA gene sequences showed that member genus Neptunomonas type naphthovorans as closest phylogenetic relative pairwise sequence...
Proteomics has been recently introduced in aquaculture research, and more methodological studies are needed to improve the quality of proteomics studies. Therefore, this work aims compare three sample preparation methods for shotgun LC-MS/MS using tissues two species: liver turbot Scophthalmus maximus hepatopancreas Mediterranean mussel Mytilus galloprovincialis. We compared most common workflows analysis: filter-aided (FASP), suspension-trapping (S-Trap), solid-phase-enhanced preparations...
Dinoflagellates in the genus Symbiodinium exhibit a variety of life styles, ranging from mutualistic endosymbioses with animal and protist hosts to free-living styles. In culture, spp. naturally associated bacteria are known form calcifying biofilms that produce so-called symbiolites, i.e., aragonitic microbialites incorporate as endolithic cells. this study, we investigated (i) how algal growth combined physiological activity these bacterial-algal associations affect physicochemical...
Oogenesis in zebrafish (Danio rerio) is controlled by the hypothalamus-pituitary-gonadal axis and reproductive hormones. In addition, an interference of stress hormones known with biology. presented work, we aimed to explore hypothesis that cortisol (Cort) ACTH may affect early oogenesis zebrafish, given presence specific receptors for glucocorticoids ovary. Follicles at stages I II were exposed vitro 1 μM Cort 48 h, then ultrastructural molecular effects analyzed. The comet assay...
To achieve more information about growth and development of oocytes in teleost fish or concerning toxicity testing, it is necessary to develop adequate vitro oocyte culture conditions. Herein, initial stages zebrafish (I, primary; II, cortical; III, vitellogenic) were analyzed under serum-free medium conditions as gonadal fragments separated oocyte-follicle complexes. Two vital dye staining methods (MTT, trypan blue) applied assess mitochondrial activity membrane integrity the during 4 days,...
The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little known about the role hormones at first stages (Stages I II) follicle in fish. In present study, zebrafish follicles Stages II were exposed vitro to reproductive 17β-oestradiol (E2), 11-ketotestosterone (11KT), 17,20β-dihydroxy-4-pregnen-3-one (DHP) secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) a concentration 1µM for 48-h period. Morphological...
Event Abstract Back to Bioactivity screening of cyanobacteria for the isolation novel anticancer compounds using 2D and 3D cell culture models Leonor Ferreira1, 2, 3*, Maria L. Sousa1, Marco Preto1, Vitor Vasconcelos1, 3 Ralph Urbatzka1* 1 Centro Interdisciplinar de Pesquisa Marine e Ambiental (CIIMAR), Portugal 2 Institute Biomedical Sciences Abel Salazar (ICBAS), Faculdade Ciências, Universidade do Porto, Cyanobacteria are microorganisms who produce secondary metabolites potentially...
Under the effect of climate change and eutrophication, cyanobacterial blooms occur in surface waters; these cyanobacteria are capable producing secondary metabolites called cyanotoxins. Among cyanotoxins, microcystins (MCs) most common harmful to human animal health. Agricultural plants come into direct contact with during irrigation waters containing cyanotoxins (MCs), whereas animals humans may across MCs following consumption initially contaminated agricultural products. The objective...