A5031591890- Bioactive Natural Diterpenoids Research
- Algal biology and biofuel production
- Drug Transport and Resistance Mechanisms
- Plant-based Medicinal Research
- Marine Sponges and Natural Products
- Essential Oils and Antimicrobial Activity
- Metabolomics and Mass Spectrometry Studies
- Microbial Natural Products and Biosynthesis
- Cancer therapeutics and mechanisms
- Medicinal Plants and Bioactive Compounds
- Natural product bioactivities and synthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Phytochemistry and Bioactivity Studies
- Marine Biology and Environmental Chemistry
- Parasitic Infections and Diagnostics
- Diatoms and Algae Research
- Plant biochemistry and biosynthesis
- Echinoderm biology and ecology
- Biocrusts and Microbial Ecology
- Biochemical and biochemical processes
- X-ray Diffraction in Crystallography
- Tea Polyphenols and Effects
- Photosynthetic Processes and Mechanisms
- Natural Compounds in Disease Treatment
- Sirtuins and Resveratrol in Medicine
Universidade do Porto
2018-2025
University of Aveiro
2021
Rede de Química e Tecnologia
2021
AlgaPlus (Portugal)
2021
University of Lisbon
2011-2020
Centro Interdisciplinar de Investigação Marinha e Ambiental
2019
Universidade Nova de Lisboa
2010
Grateloupia turuturu Yamada, 1941, is a red seaweed widely used for food in Japan and Korea which was recorded on the Atlantic Coast of Europe about twenty years ago. This presents eicosapentaenoic acid (EPA) other polyunsaturated fatty acids (PUFAs) its lipid fraction, feature that sparked interest potential applications. In seaweeds, PUFAs are mostly esterified to polar lipids, emerging as healthy phytochemicals. However, date, these biomolecules still unknown G. turuturu. The present work...
Metals are important cofactors in the metabolic processes of cyanobacteria, including photosynthesis, cellular respiration, DNA replication, and biosynthesis primary secondary metabolites. In adaptation to marine environment, cyanobacteria use metallophores acquire trace metals when necessary as well reduce potential toxicity from excessive metal concentrations. Leptochelins A–C were identified structurally novel three geographically dispersed genus Leptothoe. Determination complex...
The phytochemical study of Euphorbia piscatoria yielded jolkinol D (1) in a large amount, whose derivatization gave rise to 12 ester derivatives (2–13) and hydrolysis compound 14. vitro modulation P-gp compounds 1–14 was evaluated through combination transport chemosensitivity assays, using the L5178 mouse T lymphoma cell line transfected with human MDR1 gene. Apart from D, all (2–14) showed potential as MDR reversal agents. In this small library novel bioactive macrocyclic lathyrane...
Bacterial biofilms are complex biological systems that difficult to eradicate at a medical, industrial, or environmental level. Biofilms confer bacteria protection against external factors and antimicrobial treatments. Taking into account about 80% of human infections caused by bacterial biofilms, the eradication these structures is great priority. resistant old-generation antibiotics, which has led search for new antimicrobials from different sources, including deep oceans/seas. In this...
Lipids are one of the primary metabolites microalgae and cyanobacteria, which enrich their utility in pharmaceutical, feed, cosmetic, chemistry sectors. This work describes isolation, structural elucidation, antibiotic antibiofilm activities diverse lipids produced by different cyanobacteria strains from two European collections (ACOI LEGE-CC). Three strain were selected for antibacterial and/or activity after screening about 600 carried out under NoMorFilm project. The total organic...
Essential trace metals are vital for cellular processes such as respiration, DNA replication, and photosynthesis. Cyanobacteria must tightly regulate metal homeostasis to prevent deficiency or toxicity, yet their metallophores remain overlooked. Here, we report lusichelins A-E (1-5), new isolated from the marine cyanobacterium Lusitaniella coriacea LEGE 07167. Their structures configurational assignments were determined using NMR, mass spectrometry, TD-DFT calculations, retrobiosynthetic...
Marine organisms, particularly cyanobacteria, are important resources for the production of bioactive secondary metabolites treatment human diseases. In this study, a bioassay-guided approach was used to discover with lipid-reducing activity. Two chlorophyll derivatives were successfully isolated, previously described 132-hydroxy-pheophytin (1) and new compound 132-hydroxy-pheofarnesin (2). The structure elucidation 2 established based on one- two-dimensional (1D 2D) NMR spectroscopy mass...
Marine organisms, including seagrasses, are important sources of biologically active molecules for the treatment human diseases. In this study, organic extracts marine seagrass Halophila stipulacea obtained by different polarities from leaves (L) and stems (S) (hexane [HL, HS], ethyl acetate [EL, ES], methanol [ML, MS]) were tested bioactivities. The screening comprehended cytotoxicity activity against cancer cell lines grown as a monolayer culture or multicellular spheroids (cancer),...
Three new macrocyclic jatrophane diterpenes, named euphomelliferine (1) and euphomelliferenes A (2) B (3), one tetracyclic triterpene, 19(10→9)-abeo-8α,9β,10α-tirucalla-5,25-diene-3β,24-diol (6, C-24 epimers), were isolated from the methanolic extract of Euphorbia mellifera. known ingenane (7) two diterpenes (4 5) also isolated. Their structures elucidated by extensive spectroscopic methods, including 1D 2D homo- heteronuclear NMR experiments. Jatrophane 1-3 5 evaluated for their effects on...
Four new diterpenes were isolated from the methanolic extract of <i>Euphorbia piscatoria</i>, two <i>ent-</i>abietanes (<b>1, 2</b>) and lathyrane-type macrocyclic (<b>3, 4</b>), along with three known (<b>5</b>–<b>7</b>). Their structures characterized by spectroscopic methods, mainly 1D 2D NMR (<sup>1</sup>H, <sup>13</sup>C, DEPT, COSY, HMBC, HMQC, NOESY) experiments. Compound <b>2,</b> an unusual structure, might be considered intermediate in biosynthesis <i>ent</i>-abietane...
Epoxyboetirane A (1), a macrocyclic diterpene that was found to be inactive as an ABCB1 modulator, submitted several chemical transformations, aimed at generating series of compounds with improved multidrug resistance (MDR)-modifying activity. Overall, 23 new derivatives were prepared, in addition the already reported epoxylathyrol (2) and methoxyboetirol (3). Their anti-MDR potential assessed through both functional chemosensitivity assays on resistant human colon adenocarcinoma ABCB1-gene...
Macrocyclic monoacyl lathyrane derivatives bearing a benzoyl moiety were previously found to be strong ABCB1 modulators. To explore the effects of different substituents aromatic moiety, 14 new compounds (1.1-1.7, 1.10, and 2.1-2.4) prepared from jolkinol D (1), obtained Euphorbia piscatoria, jolkinodiol (2), its hydrolysis derivative. Compounds 1.8 1.9, having aliphatic moieties, also obtained. The reversal ABCB1-mediated MDR was evaluated through functional chemosensitivity assays on human...
Four natural lactylates of chlorinated fatty acids, chlorosphaerolactylates A–D (1–4), were isolated from the methanolic extract cyanobacterium Sphaerospermopsis sp. LEGE 00249 through a combination bioassay-guided and MS-guided approaches. Compounds 1–4 are esters (mono-, di-, or tri)chlorinated lauric acid lactic acid, whose structures assigned on basis spectrometric spectroscopic methods inclusive 1D 2D NMR experiments. High-resolution mass-spectrometry data sets also demonstrated...
Multidrug resistance (MDR) has been regarded as one of the major hurdles for successful outcome cancer chemotherapy. The collateral sensitivity (CS) effect is most auspicious anti-MDR strategies. Epoxylathyrane derivatives 1-16 were obtained by derivatization macrocyclic diterpene epoxyboetirane A (17), a lathyrane-type isolated from Euphorbia boetica. Some these compounds found to strongly modulate P-glycoprotein (P-gp/ABCB1) efflux.The main goal was develop diterpenes with improved...
The Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) holds a vast number of cyanobacteria whose chemical richness is still largely unknown. To expedite its bioactivity screening we developed natural products library. Sixty strains four environmental samples were chromatographed, using semiautomatic HPLC system, yielding 512 fractions that tested for their cytotoxic activity against 2D 3D models human colon carcinoma (HCT 116), non-cancerous cell line hCMEC/D3. Six showed...
Multidrug resistance (MDR) is a limiting step on the success of cancer chemotherapy. The drug efflux mediated by P-gp (Pglycoprotein) one best studied mechanisms MDR. This paper focuses inhibitory activity, pharmacophore modeling and structure-activity relationships studies sixteen macrocyclic diterpenes polycyclic derivatives obtained from Euphorbia species. MDR human colon adenocarcinoma cells (COLO 320 MDR) overexpressing were used as biological model to screen for dependent inhibitors....