A5078246040- Nanoparticle-Based Drug Delivery
- Genetic Neurodegenerative Diseases
- Advanced Electron Microscopy Techniques and Applications
- Protein Structure and Dynamics
- Image Processing Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Caveolin-1 and cellular processes
- Retinal Development and Disorders
- Reproductive biology and impacts on aquatic species
- Alzheimer's disease research and treatments
- Genomics and Chromatin Dynamics
- Sperm and Testicular Function
- Pesticide Residue Analysis and Safety
- Advanced NMR Techniques and Applications
- Nuclear Structure and Function
- Domain Adaptation and Few-Shot Learning
- Microtubule and mitosis dynamics
- Mesoporous Materials and Catalysis
- Environmental Toxicology and Ecotoxicology
- Pharmaceutical and Antibiotic Environmental Impacts
- Protein Kinase Regulation and GTPase Signaling
- Advanced Drug Delivery Systems
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2025
Universidade do Porto
2011-2023
Instituto de Biologia Molecular e Celular
2015-2023
International Iberian Nanotechnology Laboratory
2020-2022
Xanthomonas axonopodis pv. citri is the causal agent of citrus canker, which one most serious diseases citrus. To understand virulence mechanisms X. citri, we designed and conducted genome-wide microarray analyses to characterize HrpG HrpX regulons, are critical for pathogenicity citri. Our revealed that 232 181 genes belonged respectively. In total, 123 were overlapped in two regulons at any three selected timepoints representing growth stages XVM2 medium. results showed regulated all 24...
Intervertebral disc (IVD) degeneration is characterized by significant biochemical and histomorphological alterations, such as loss of extracellular matrix (ECM) integrity, abnormal synthesis ECM main components, resultant from altered anabolic/catabolic cell activities death. Mesenchymal Stem/Stromal Cell (MSC) migration towards degenerated IVD may represent a viable strategy to promote tissue repair/regeneration. Here, human MSCs (hMSCs) were seeded on top cartilaginous endplates (CEP)...
Spinocerebellar ataxia type 3 (SCA3) is a rare inherited neurodegenerative disease caused by the expansion of polyglutamine repeat in protease ataxin-3 (Atx3). Despite extensive knowledge downstream pathophysiology, no disease-modifying therapies are currently available to halt progression. The accumulation protein inclusions enriched polyQ-expanded Atx3 neurons suggests that inhibiting its self-assembly may yield targeted therapeutic approaches. Here it shown supramolecular tweezer, CLR01,...
An analytical method based on solid-phase extraction followed by gas chromatography mass spectrometry (GC-MS) was developed and validated for the quantification of 39 pesticides with distinct physico-chemical characteristics (including some degradates), in estuarine water samples. The includes pre-concentration samples (2500-fold factor), OASIS HLB cartridges, solid phase extraction. analysed included organochlorines, organophosphorous, triazines, pyrethroids other miscellaneous compounds,...
This study focusses on the development of a magnetic zeolite nanocomposite as suitable platform towards design theranostic system. Herein, we explored its ability to act <italic>T</italic><sub>2</sub>-MRI contrast enhancer when nanoparticles are incorporated in structure.
The Rho GTPase Rac1 is a multifunctional protein involved in distinct pathways ranging from development to pathology. aim of the present study was unravel contribution neuronal regulating response brain injury induced by permanent focal cerebral ischemia (pMCAO). Our results show that pMCAO significantly increased total levels wild type mice, mainly through rising nuclear Rac1, while reduction activation observed. Such changes preceded cell death excitotoxic stress. Pharmacological...
Zeolites are crystalline porous materials with a regular framework which have non-toxic effects on variety of human cell lines and been explored for imaging drug delivery. Understanding the interaction between zeolite nanoparticles cells is imperative improving their potentialities, since process internalization these particles still poorly understood. In this study, intracellular trafficking kinetics L into breast cancer normal epithelial mammary were analysed using scanning electron...
Spinocerebellar ataxia type 3 (SCA3) is caused by the expansion of a glutamine repeat in protein ataxin-3, which deposited as intracellular aggregates affected brain regions. Despite controversial role ataxin-3 amyloid structures SCA3 pathology, identification molecules with capacity to prevent aberrant self-assembly and stabilize functional conformation(s) key development therapeutic solutions. Amyloid-specific kinetic assays are routinely used measure rates vitro employed during screening...
Oogenesis in zebrafish (Danio rerio) is controlled by the hypothalamus-pituitary-gonadal axis and reproductive hormones. In addition, an interference of stress hormones known with biology. presented work, we aimed to explore hypothesis that cortisol (Cort) ACTH may affect early oogenesis zebrafish, given presence specific receptors for glucocorticoids ovary. Follicles at stages I II were exposed vitro 1 μM Cort 48 h, then ultrastructural molecular effects analyzed. The comet assay...
The accumulation of mutant ataxin-3 (Atx3) in neuronal nuclear inclusions is a pathological hallmark Machado-Joseph disease (MJD), also known as Spinocerebellar Ataxia Type 3. Decreasing the protein aggregation burden possible disease-modifying strategy to tackle MJD and other neurodegenerative disorders for which only symptomatic treatments are currently available. We performed drug repurposing screening identify inhibitors Atx3 with toxicological pharmacokinetic profiles. Interestingly,...
The throughput level currently reached by automatic liquid handling and assay monitoring techniques is expected to facilitate the discovery of new modulators enzyme activity. Judicious dependable ways interpret vast amounts information are, however, required effectively answer this challenge. Here, 3-point method kinetic analysis proposed as a means significantly increase hit success rates decrease number falsely identified compounds (false positives). In post-Michaelis-Menten approach, each...
The assembly of biomolecular condensate in eukaryotic cells and the accumulation amyloid deposits neurons are processes involving nucleation growth (NAG) new protein phases. To therapeutically target phase separation, drug candidates tested vitro assays that monitor increase mass or size phase. Limited mechanistic insight is, however, provided if empirical untestable kinetic models fitted to these progress curves. Here we present web server NAGPKin quantifies NAG rates using mass-based...
Abstract The accumulation of mutant ataxin-3 (Atx3) in neuronal nuclear inclusions is a pathological hallmark Machado-Joseph disease (MJD), also known as Spinocerebellar Ataxia Type 3. Decreasing the protein aggregation burden possible disease-modifying strategy to tackle MJD and other neurodegenerative disorders for which only symptomatic treatments are currently available. We performed drug repurposing screening identify inhibitors Atx3 with toxicological pharmacokinetic profiles....
Aluminum (Al) toxicity limits crops growth and production in acidic soils. Compared to roots, less is known about the toxic effects of Al leaves. subcellular compartmentalization also largely unknown. Using rye ( Secale cereale L.) genotypes differing tolerance, we evaluated patterns accumulation leaf cell organelles photosynthetic metabolic strategies cope with among Beira (more tolerant) RioDeva sensitive) genotypes. Al-tolerant genotype accumulated all organelles, except vacuoles. This...