A5063472068- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Receptor Mechanisms and Signaling
- Synthetic Organic Chemistry Methods
- Protein Kinase Regulation and GTPase Signaling
- Seaweed-derived Bioactive Compounds
- Fungal Biology and Applications
- Chemical synthesis and alkaloids
- Synthesis and Biological Activity
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Natural product bioactivities and synthesis
- Bioactive Compounds and Antitumor Agents
- Glycosylation and Glycoproteins Research
- Neuropeptides and Animal Physiology
- Marine and coastal plant biology
- Traditional and Medicinal Uses of Annonaceae
- Carbohydrate Chemistry and Synthesis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- Microbial Community Ecology and Physiology
- Molecular spectroscopy and chirality
- Phytochemistry and Biological Activities
University of Bonn
2015-2024
Max Planck Institute for Neurobiology of Behavior – caesar
2023
Max Planck Institute of Neurobiology
2023
Center of Advanced European Studies and Research
2023
German Center for Infection Research
2014-2022
Pharmac
2021
Kaspe (Czechia)
2020
University of Würzburg
2012
University of Khartoum
2008-2010
Helmholtz Centre for Infection Research
2010
G protein-independent, arrestin-dependent signaling is a paradigm that broadens the scope of protein-coupled receptors (GPCRs) beyond proteins for numerous biological processes. However, arrestin in collective absence functional has never been demonstrated. Here we achieve state "zero G" at cellular level using HEK293 cells depleted by CRISPR/Cas9 technology Gs/q/12 families Gα proteins, along with pertussis toxin-mediated inactivation Gi/o. Together lacking β-arrestins ("zero arrestin"),...
Abstract Despite the discovery of heterotrimeric αβγ G proteins ∼25 years ago, their selective perturbation by cell-permeable inhibitors remains a fundamental challenge. Here we report that plant-derived depsipeptide FR900359 (FR) is ideally suited to this task. Using multifaceted approach systematically characterize FR as inhibitor Gq/11/14 over all other mammalian Gα isoforms and elaborate its molecular mechanism action. We also use investigate whether inhibition Gq an effective...
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Replacement of the lost myelin sheath is a therapeutic goal for treating demyelinating diseases central nervous system (CNS), such as multiple sclerosis (MS). The G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptor (GPCR) GPR17, which phylogenetically closely related to receptors "purinergic cluster," has emerged modulator CNS myelination. However, whether GPR17-mediated signaling positively or negatively regulates this critical process unresolved. We identified...
The ability of cyanobacteria to produce complex secondary metabolites with potent biological activities has gathered considerable attention due their potential therapeutic and agrochemical applications. However, the precise physiological or ecological roles played by a majority these have remained elusive. Several studies shown that are able interfere other organisms in communities through release compounds into surrounding medium, phenomenon usually referred as allelopathy. Exudates from...
Inhibition of constitutively activated, oncogenic G proteins inhibits uveal melanoma tumor growth in mice.
Brain capillary calcium signaling controls blood flow.
The marine sponges Ectyplasia perox and Myxilla incrustans were investigated for associated fungal strains. Among others, a Coniothyrium sp., from E. perox, Microsphaeropsis M. incrustans, isolated, cultured, their biologically active secondary metabolite contents. new compound microsphaeropsisin (1) together with the known compounds (R)-mellein (4), (3R,4S)-hydroxymellein (5), (3R,4R)-hydroxymellein (6), 4,8-dihydroxy-3,4-dihydro-2H-naphthalen-1-one (7) isolated sp. From culture extracts of...
From the green alga Ulva sp., endophytic and obligate marine fungus Ascochyta salicorniae was isolated. A. mass cultivated found to produce unprecedented structurally unusual tetramic acid containing metabolites ascosalipyrrolidinones A (1) B (2). Additionally, new natural product ascosalipyrone (3) known 4 5 were obtained. Ascosalipyrrolidinone has antiplasmodial activity toward Plasmodium falciparum strains K1 NF 54, as well showing antimicrobial inhibiting tyrosine kinase p56lck.
From the CH2Cl2 extract of temperate marine red alga, Laurencia rigida, which has antifouling properties, eight sesquiterpenes (1−8) were isolated. Of these, four (3-acetoxy-E-γ-bisabolene (1), (−)-10α-bromo-9β-hydroxy-α-chamigrene (2), rigidol (3), and (+)-(10S)-10-bromo-β-chamigrene (4)), shown to be new natural products. For known compound deschloroelatol (5), reassignment 1H- 13C-NMR data was found necessary on basis extensive NMR measurements. elatol (6), complete are also reported. The...
A total of 80 lipophilic and hydrophilic extracts obtained from 20 samples cultured freshwater terrestrial cyanobacteria were investigated for their biological activities. Of all the extracts, 26% exhibited a significant lethal effect against brine shrimp. Out 54 tested antimicrobial activity, 78% showed antibacterial 45% antifungal 30 cytotoxicity KB cells, none was found to be active. Bioassay-guided fractionation Fischerella ambigua led isolation three compounds; ambigols (1) B (2),...
The marine fungus Apiospora montagnei was isolated from the inner tissue of North Sea alga Polysiphonia violacea. Cultivation this fungal strain led to isolation several new secondary metabolites, including diterpene myrocin A (1) and polyketide apiosporic acid (2). Furthermore monomethyl ester 9-hydroxyhexylitaconic (3) (−)-enantiomer (4) known (+)-hexylitaconic were found together with (+)-epiepoxydon (5), (+)-epoxydon monoacetate, R-mellein, R-8-methoxymellein,...
Investigation of the fungal strain Monodictys putredinis isolated from inner tissue a marine green alga led to isolation four new monomeric xanthones and benzophenone. All structures were elucidated by extensive spectroscopic measurements. The relative configuration compound 1 was determined X-ray crystal structure analysis, while for 2 3 configurations confirmed NOE experiments. Absolute compounds 1−3 deduced comparing experimental circular dichroism data with those calculated employing...
The marine-derived fungus Coniothyrium cereale was isolated from the green alga Enteromorpha sp. and found to produce new phenalenone derivatives 1-7 as well known compounds lactone 8, (-) sclerodin (9), lamellicolic anhydride (10), scleroderolide (11), sclerodione (12). structures of these closely related were established extensive spectroscopic investigations on basis one two dimensional NMR studies ((1)H, (13)C, COSY, NOESY, HSQC HMBC) mass spectrometric analysis (LC/MS, HREIMS HRESIMS),...
Abstract Corallopyronin A is a myxobacterial compound with potent antibacterial activity. Feeding experiments labelled precursors resulted in the deduction of all biosynthetic building blocks for corallopyronin and revealed an unusual feature this metabolite: its biosynthesis from two chains, one solely PKS‐derived other NRPS/PKS‐derived. The starter molecule believed to be carbonic acid or monomethyl ester. putative gene cluster trans‐AT‐type mixed PKS/NRPS cluster, containing β‐branching...