A5087136590- Microbial Community Ecology and Physiology
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Polar Research and Ecology
- Plant biochemistry and biosynthesis
- Marine Sponges and Natural Products
- Microbial Metabolism and Applications
- Protist diversity and phylogeny
- Corrosion Behavior and Inhibition
- Biosensors and Analytical Detection
- Actinomycetales infections and treatment
- Marine Biology and Ecology Research
- Marine Biology and Environmental Chemistry
- Plant and animal studies
- Microbial bioremediation and biosurfactants
- Marine Ecology and Invasive Species
- SARS-CoV-2 detection and testing
- Photosynthetic Processes and Mechanisms
- Carbohydrate Chemistry and Synthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Bacteriophages and microbial interactions
Universidade do Porto
2016-2025
Abstract With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role the discovery novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these are often organised into gene clusters (BGCs). In 2015, we defined Minimum Information about Biosynthetic Gene cluster (MIBiG): standardised format describes minimally required information to...
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with development of High Throughput Sequencing (HTS) techniques. However, some remains inaccessible by power sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria cyanobacteria along different soil endolithic micro-environments Victoria Valley Valleys. Our results demonstrate that distribution is driven environmental forces,...
Terpenes, a large family of natural products with important applications, are commonly associated plants and fungi. The diterpenoids dehydroabietic abietic acids defense metabolites abundant in resin, used as biomarkers for conifer plants. We report here the first time that two diterpenoid produced by members several genera cyanobacteria. Dehydroabietic acid was isolated from cyanobacterial strains its identity confirmed spectroscopically. One or both were detected cells phylogenetically...
Marine biofilms are known to influence the corrosion of metal surfaces in marine environment. Despite some recent research, succession bacterial communities colonizing artificial remains uncharacterized temporal settings. More specifically, it is not fully if colonizers similar or distinct different seasons year. In particular study early biofilms, which cells first adhere surfaces, crucial for development subsequent biofilm communities. this work, we used amplicon-based NGS (next-generation...
Recent analyses of genome data indicate that members the cyanobacterial order Pleurocapsales show tremendous potential for natural product discovery. However, only a few compounds have been reported from this order. Here, we report isolation hyellamide (1), glycosylated N-acyl tyrosine-derived eneamide, pleurocapsalean cyanobacterium Hyella patelloides LEGE 07179. The putative biosynthetic gene cluster 1 was identified in producing organism and proposal is presented. This work sheds light on...
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) two pharmaceutically important families NPs recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) peptide synthetase (NRPS) genes, likely be involved in the production novel metabolites. Despite this, diversity secondary metabolites genes is poorly explored. In this study, computational bioprospection approach was employed study...
Polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) are mega enzymes responsible for the biosynthesis of a large fraction natural products (NPs). Molecular markers biosynthetic genes, such as ketosynthase (KS) domain PKSs, have been used to assess diversity distribution genes in complex microbial communities. More recently, metagenomic studies complemented enhanced this approach by allowing recovery complete gene clusters (BGCs) from environmental DNA. In study, Arctic...
The monitoring of cities' wastewaters for the detection potentially pathogenic viruses and bacteria has been considered a priority during COVID-19 pandemic to monitor public health in urban environments. methodological approaches frequently used this purpose include deoxyribonucleic acid (DNA)/Ribonucleic (RNA) isolation followed by quantitative polymerase chain reaction (qPCR) reverse transcription (RT)‒qPCR targeting genes. More recently, application metatranscriptomic opened opportunities...
Two novel actinomycetal strains, designated CC-R113 T and CC-R104 , were isolated from the tissues of two macroalgae collected on northern Portuguese coast. Phylogenetic analyses based 16S rRNA gene showed that strain CT-R113 belongs to genus Nocardiopsis being closely related umidischolae 66/93 tropica VKM Ac-1457 with 98.65 98.39 % sequence similarity, respectively. The clade formed between three type strains was confirmed by phylogenomic analysis. genome 7.27 Mb in size a G+C content 71.3...
Abstract Background Halogenation is a recurring feature in natural products, especially those from marine organisms. The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. Recently, CylC – the first predicted dimetal-carboxylate halogenase to be characterized was shown regio- and stereoselectively install chlorine atom onto an unactivated carbon center during cylindrocyclophane biosynthesis. Homologs of are...
The morphology, 16S rRNA gene phylogeny and 16S-23S ITS secondary structures of three strains marine Cyanobacteria, isolated from inter- subtidal environments north Portugal were studied, resulting in the description Zarconia navalis gen. nov., sp. nov. (Oscillatoriales incertae sedis), Romeriopsis (Leptolyngbyaceae) marina named under International Code Nomenclature for algae, fungi, plants. No diacritical morphological characters found new genera species. maximum-likelihood Bayesian...
The McMurdo Dry Valleys constitute the largest ice-free region of Antarctica and one most extreme deserts on Earth. Despite low temperatures, dry poor soils katabatic winds, some microbes are able to take advantage endolithic microenvironments, inhabiting pore spaces soil constituting photosynthesis-based communities. We isolated a green microalga, Endolithella mcmurdoensis gen. et sp. nov, from an sandstone sample collected in (Victoria Land, East Antarctica) during K020 expedition, January...
ABSTRACT Actinomycetota are unrivalled producers of bioactive natural products, with strains living in association macroalgae representing a prolific—yet largely unexplored—source specialised chemicals. In this work, we have investigated the potential from through culture‐dependent and ‐independent approaches. A bioprospecting pipeline was applied to collection 380 actinobacterial strains, recovered two species collected Portuguese northern shore— Codium tomentosum Chondrus crispus— order...
Abstract Halogenation is a recurring feature in natural products, especially those from marine organisms. The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. Recently, CylC – the first predicted dimetal-carboxylate halogenase to be characterized was shown regio- and stereoselectively install chlorine atom onto an unactivated carbon center during cylindrocyclophane biosynthesis. Homologs of are also found...