A5038317651- Algal biology and biofuel production
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Microbial Community Ecology and Physiology
- Polar Research and Ecology
- Biocrusts and Microbial Ecology
- Marine Sponges and Natural Products
- Plant biochemistry and biosynthesis
- Protist diversity and phylogeny
- Microbial Metabolism and Applications
- Porphyrin and Phthalocyanine Chemistry
- Antioxidant Activity and Oxidative Stress
- Aquatic Ecosystems and Phytoplankton Dynamics
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Tannin, Tannase and Anticancer Activities
- Chemical synthesis and alkaloids
- Marine and coastal ecosystems
Universidade do Porto
2018-2025
Centro Interdisciplinar de Investigação Marinha e Ambiental
2022
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with development of High Throughput Sequencing (HTS) techniques. However, some remains inaccessible by power sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria cyanobacteria along different soil endolithic micro-environments Victoria Valley Valleys. Our results demonstrate that distribution is driven environmental forces,...
To address the on-going need for chemical novelty and limited information on Planctomycetota secondary metabolism, we focused exploring recently isolated marine planctomycetal strain Alienimonas chondri LzC2T to uncover its potential production of novel compounds. This work contemplates description a large-scale cultivation study LzC2T, followed by metabolite extraction compound isolation using chromatographic approaches, which resulted in molecule designated as alichondrichlorin. Structural...
Recent analyses of genome data indicate that members the cyanobacterial order Pleurocapsales show tremendous potential for natural product discovery. However, only a few compounds have been reported from this order. Here, we report isolation hyellamide (1), glycosylated N-acyl tyrosine-derived eneamide, pleurocapsalean cyanobacterium Hyella patelloides LEGE 07179. The putative biosynthetic gene cluster 1 was identified in producing organism and proposal is presented. This work sheds light on...
In recent years, extensive sequencing and annotation of bacterial genomes has revealed an unexpectedly large number secondary metabolite biosynthetic gene clusters whose products are yet to be discovered. For example, cyanobacterial contain a variety that likely incorporate fatty acid derived moieties, but for most cases we lack the knowledge tools effectively predict or detect encoded natural products. Here, exploit apparent absence functional β-oxidation pathway in cyanobacteria achieve...
Bioprospecting actinobacterial secondary metabolism from untapped marine sources may lead to the discovery of biotechnologically-relevant compounds. While studying diversity and bioactive potential Actinomycetota associated with Codium tomentosum, a green seaweed collected in northern Portuguese cost, strain CT-F61, identified as Streptomyces violaceoruber , was isolated. Its extracts displayed strong anticancer activity on breast carcinoma T-47D colorectal HCT116 cells, being effective well...
Nocuolin A is a cytotoxic cyanobacterial metabolite that proposed to be produced by enzymes of the noc biosynthetic gene cluster. features 1,2,3-oxadiazine moiety, structural feature unique among natural products and, so far, inaccessible through organic synthesis, suggesting novel enzymatic chemistry might involved in its biosynthesis. This heterocycle substituted with two alkyl chains and 3-hydroxypropanoyl moiety. We report here our efforts elucidate origin carbon skeleton nocuolin A....
The McMurdo Dry Valleys constitute the largest ice-free region of Antarctica and one most extreme deserts on Earth. Despite low temperatures, dry poor soils katabatic winds, some microbes are able to take advantage endolithic microenvironments, inhabiting pore spaces soil constituting photosynthesis-based communities. We isolated a green microalga, Endolithella mcmurdoensis gen. et sp. nov, from an sandstone sample collected in (Victoria Land, East Antarctica) during K020 expedition, January...
Fatty acyl moieties are a very common feature of cyanobacterial secondary metabolites. Using recently developed method to discover fatty acyl-containing natural products, we detected and isolated three new lipopeptides – fisch-erazoles A-C (1-3) from the cyanobacterium Fischerella sp. PCC 9431. These metabolites present extensive halo-genation in their moiety as well an unusual pendant allyl alcohol. We analyzed genome sequence producing identified putative biosynthetic gene cluster...
Apocarotenoids are widely distributed among living organisms (bacteria, fungi, algae, plants and even animals) have been associated with several signaling functions. These compounds generated by the activity of carotenoid cleavage dioxygenases (CCDs), whose diversity greatly contributes to large number apocarotenoids that described so far. It is nevertheless expected a considerable these molecules yet be discovered. In this work, we describe isolation structural elucidation apocarotenoid...
Natural products have an important role in several human activities, most notably as sources of new drugs. In recent years, massive sequencing and annotation bacterial genomes has revealed unexpectedly large number secondary metabolite biosynthetic gene clusters whose are yet to be discovered. For example, cyanobacterial contain a that likely incorporate fatty acid-derived moieties, but for cases we lack the knowledge tools effectively predict or detect encoded natural products. Here,...
Abstract In recent years, extensive sequencing and annotation of bacterial genomes has revealed an unexpectedly large number secondary metabolite biosynthetic gene clusters whose products are yet to be discovered. For example, cyanobacterial contain a variety that likely incorporate fatty acid derived moieties, but for most cases we lack the knowledge tools effectively predict or detect encoded natural products. Here, exploit apparent absence functional β‐oxidation pathway in cyanobacteria...
Natural products have an important role in several human activities, most notably as sources of new drugs. In recent years, massive sequencing and annotation bacterial genomes has revealed unexpectedly large number secondary metabolite biosynthetic gene clusters whose are yet to be discovered. For example, cyanobacterial contain a that likely incorporate fatty acid-derived moieties, but for cases we lack the knowledge tools effectively predict or detect encoded natural products. Here,...
Natural products have an important role in several human activities, most notably as sources of new drugs. In recent years, massive sequencing and annotation bacterial genomes has revealed unexpectedly large number secondary metabolite biosynthetic gene clusters whose are yet to be discovered. For example, cyanobacterial contain a that likely incorporate fatty acid-derived moieties, but for cases we lack the knowledge tools effectively predict or detect encoded natural products. Here,...
Nocuolin A is a cytotoxic cyanobacterial metabolite that proposed to be produced by enzymes of the noc biosynthetic gene cluster. features 1,2,3-oxadiazine moiety, structural feature unique among natural products and, so far, inaccessible through organic synthesis, suggesting novel enzymatic chemistry might involved in its biosynthesis. This heterocycle substituted with two alkyl chains and 3-hydroxypropanoyl moiety. We report here our efforts elucidate origin carbon skeleton nocuolin A....