Miguel Semedo

ORCID: 0000-0003-2263-2212
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About
Contact & Profiles
Research Areas
  • Microbial Community Ecology and Physiology
  • Wastewater Treatment and Nitrogen Removal
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Aquaculture disease management and microbiota
  • SARS-CoV-2 detection and testing
  • Protist diversity and phylogeny
  • Environmental DNA in Biodiversity Studies
  • Biosensors and Analytical Detection
  • Soil and Water Nutrient Dynamics
  • Environmental Toxicology and Ecotoxicology
  • SARS-CoV-2 and COVID-19 Research
  • Methane Hydrates and Related Phenomena
  • Marine and coastal ecosystems
  • Mercury impact and mitigation studies
  • Bacteriophages and microbial interactions
  • Parasite Biology and Host Interactions
  • Genomics and Phylogenetic Studies
  • Polar Research and Ecology
  • Toxic Organic Pollutants Impact
  • Agriculture, Soil, Plant Science
  • Advanced biosensing and bioanalysis techniques
  • Climate Change Communication and Perception
  • Animal testing and alternatives
  • Effects and risks of endocrine disrupting chemicals
  • Aquaculture Nutrition and Growth

Universidade do Porto
2012-2025

William & Mary
2018-2022

Centro Interdisciplinar de Investigação Marinha e Ambiental
2022

Antibiotics in soils may affect the structure and function of microbial communities. In this study, we investigated effects tetracycline on soil community composition production nitrous oxide (N2O) dinitrogen (N2) as end-products denitrification. Grassland were pre-incubated with without for one-week prior to measurements N2O N2 slurries along analysis prokaryotic fungal communities by qPCR next-generation sequencing. Abundance taxonomical bacteria carrying two genotypes reductase genes...

10.3389/fmicb.2018.02121 article EN cc-by Frontiers in Microbiology 2018-09-11

Oil spills are among the most catastrophic events to marine ecosystems and current remediation techniques not suitable for ecological restoration. Bioremediation approaches can take advantage of activity microorganisms with biodegradation capacity thus helping accelerate recovery contaminated environments. The use native increase bioremediation efficiency since they have higher potential survive in natural environment while preventing unpredictable impacts associated introduction non-native...

10.3389/fmicb.2021.633659 article EN cc-by Frontiers in Microbiology 2021-04-23

ABSTRACT The extensive microbial diversity found in the oceans is becoming to be uncovered despite limited knowledge and cultured representatives for many taxonomic groups. This study analysed distribution of Planctomycetota at four water column profiles Eastern North Pacific subtropical front (ENPSF) using 16S rRNA gene sequencing. A dual approach, utilising PacBio long‐reads Illumina short‐reads, was employed enhance accuracy assignment compare sequencing methods. increased below deep...

10.1111/1758-2229.70063 article EN cc-by Environmental Microbiology Reports 2025-02-01

Nitrification is a vital ecosystem function in the open ocean that regenerates inorganic nitrogen and promotes primary production. Recent studies have shown ecology physiology of nitrifying organisms more complex than previously postulated. The distribution these remote oligotrophic their interactions with physicochemical environment are relatively understudied. In this work, we aimed to evaluate depth profile archaea bacteria Eastern North Pacific Subtropical Front, an area limited...

10.3389/fmicb.2021.624071 article EN cc-by Frontiers in Microbiology 2021-02-23

Recirculating aquaculture systems (RAS) pose unique challenges in microbial community management since they rely on a stable with key target groups, both the RAS environment and host (in this case, Solea senegalensis). Our goal was to determine how much of sole microbiome is inherited from egg stage, acquired during remainder life cycle an production batch, especially regarding potentially probiotic pathogenic groups. work comprises tissue samples 2 days before hatching up 146 after (-2...

10.3389/fmicb.2023.1188876 article EN cc-by Frontiers in Microbiology 2023-06-26

The intensification of the poultry industry in last decades has led to a sharp increase number animal processing plants discharging wastewater water bodies. These discharges may have significant effect on environmental quality and important ecosystem functions, such as denitrification. We conducted seasonal survey microcosm experiment an impacted reference tidal creek investigate impacts discharge from plant sedimentary microbial communities, denitrification activity, nitrate removal....

10.1021/acs.est.9b03560 article EN Environmental Science & Technology 2019-12-11

The intensification of the poultry industry may lead to increased spread antibiotic resistance genes (ARGs) in environment. However, impacts wastewater discharge from processing plants on sediment resistome are relatively unexplored. Furthermore, its relationships with important biogeochemical pathways, such as N cycle, virtually unknown. overall objective this study was examine abundance and diversity cycling microbial communities impacted by wastewater. We performed a metagenomic...

10.1016/j.scitotenv.2022.159496 article EN cc-by-nc-nd The Science of The Total Environment 2022-10-17

ABSTRACT Nitrous oxide (N2O) is a potent greenhouse gas and its reduction to dinitrogen by the N2O reductase (encoded nosZ gene) only known biological sink. Within phylogeny there are two major clades (I II), which seem have different ecological niches. However, physiological differences of nosZI nosZII expression that may impact emissions not well understood. Here, we evaluated differential nosZII, both present in Thauera linaloolentis strain 47LolT, response concentration presence...

10.1093/femsle/fnaa205 article EN FEMS Microbiology Letters 2020-12-01

The monitoring of cities' wastewaters for the detection potentially pathogenic viruses and bacteria has been considered a priority during COVID-19 pandemic to monitor public health in urban environments. methodological approaches frequently used this purpose include deoxyribonucleic acid (DNA)/Ribonucleic (RNA) isolation followed by quantitative polymerase chain reaction (qPCR) reverse transcription (RT)‒qPCR targeting genes. More recently, application metatranscriptomic opened opportunities...

10.1016/j.envres.2023.116040 article EN cc-by Environmental Research 2023-05-05

Microbial communities are crucial for important ecosystem functions in the open ocean, such as primary production and nutrient cycling. However, few studies have addressed distribution of microplankton remote oligotrophic region Pacific Ocean. Moreover, biogeochemical physical drivers microbial community structure not fully understood these areas. This research aims to investigate patterns prokaryotic protists communities’ North Subtropical Front (NPSF). The NPSF is a vast with layered...

10.3389/fmicb.2024.1455196 article EN cc-by Frontiers in Microbiology 2024-12-24

Keywords: precipitation, aerosols, microorganisms, Antarctica, cloudsClouds and precipitation play an intrinsic role in the global climate, upholding Earth's surface energy equilibrium water cycle. Despite their significance, clouds aerosols over Antarctica Southern Ocean remain poorly understood, primarily due to extreme environment for observations insufficient data. The Antarctic Peninsula (AP) has been exhibiting a significant warming trend last 60 years (Jones et al, 2019). Coupled with...

10.5194/egusphere-egu24-18750 preprint EN 2024-03-11

Abstract Research on the emerging COVID-19 pandemic is demonstrating that wastewater infrastructures can be used as public health observatories of virus circulation in human communities. Important efforts are being organized worldwide to implement sewage-based surveillance SARS-CoV-2 for preventive or early warning purposes, informing preparedness and response measures. However, its successful implementation requires important iterative methodological improvements, well establishment...

10.1101/2021.04.06.21254994 preprint EN cc-by-nc-nd medRxiv (Cold Spring Harbor Laboratory) 2021-04-09

The intensification of the poultry industry may lead to increased spread antibiotic resistance genes (ARGs) in environment. However, impacts wastewater discharge from processing plants on sediment resistome are relatively unexplored. Furthermore, its relationships with important biogeochemical pathways, such as N cycle, virtually unknown. overall objective this study was examine abundance and diversity cycling microbial communities impacted by wastewater. We performed a metagenomic...

10.2139/ssrn.4158230 article EN SSRN Electronic Journal 2022-01-01

Prokaryotic interactions in recirculating aquaculture systems (RAS) environmental communities may play a crucial role driving their functional potential. However, these are often neglected. The aim of this work is to detect prokaryotic Solea senegalensis RAS, through the definition relevant taxa and cluster identification using co-variance co-occurrence tools. Amplicon sequence variants (ASV) were obtained from water, tank biofilm, biofilters two systems, pre-ongrowing weaning, study focuses...

10.3389/fmars.2022.1038196 article EN cc-by Frontiers in Marine Science 2022-12-01

Deep-sea mining may lead to the release of high concentrations metals into surrounding seabed, which can disturb important ecosystem functions provided by microbial communities. Among these, production N2O and its reduction N2 is great relevance since an greenhouse gas. Metal impacts on net deep-sea bacteria are, however, currently unexplored. Here, we evaluated effects cadmium (Cd) a isolate, Shewanella loihica PV-4. We performed series Cd exposure incubations in oxic conditions determined...

10.1093/femsle/fnad047 article EN cc-by-nc FEMS Microbiology Letters 2023-01-01
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