A5052431324- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Reproductive biology and impacts on aquatic species
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Genetic and rare skin diseases.
- Vibrio bacteria research studies
- Ichthyology and Marine Biology
- Myxozoan Parasites in Aquatic Species
- Food and Agricultural Sciences
- Protein Hydrolysis and Bioactive Peptides
- Immune Response and Inflammation
- Congenital heart defects research
- Genetic diversity and population structure
- interferon and immune responses
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquatic life and conservation
- Microbial infections and disease research
Agri-Food and Veterinary Authority of Singapore
2024
James Cook University Singapore
2024
Temasek Life Sciences Laboratory
2013-2020
National University of Singapore
2014-2015
Nanyang Technological University
2013-2015
We report here the ~670 Mb genome assembly of Asian seabass (Lates calcarifer), a tropical marine teleost. used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive chromosome-level contig N50 size over 1 scaffold 25 that span ~90% genome. The population structure L. calcarifer complex was analyzed re-sequencing 61 individuals representing various regions across species’ native range. SNP analyses...
Vaccination is an important strategy in the protection of aquaculture species from major diseases. However, we still do not have a good understanding mechanisms underlying vaccine-induced disease resistance. This further complicated by presence several lymphoid organs that play different roles when mounting immune response. In this study, attempt to elucidate some these using microarray-based approach. Asian seabass (Lates calcarifer) were vaccinated against Streptococcus iniae and...
Abstract Asian arowana or dragonfish ( Scleropages formosus ) is an important fish species due to its unusual breeding biology and high economic value in the ornamental markets. In present study, we aimed (i) create first transcriptome by Roche 454 pyrosequencing of brain gonad samples; (ii) identify differentially expressed genes between two sexes develop microsatellite (SSR) markers; (iii) construct a first‐generation SSR‐based genetic linkage map. A total over 1.3 million reads were...
As nutrition-related expenses constitute the majority of costs for aquaculture farms, it is essential them to use feeds that provide an ideal combination nutrients species choice. In this study, relative effect consuming three different pelleted (B, C and D) in comparison frozen baitfish (A; control) were compared on juvenile Asian seabass (77.3 ± 22.4g) selected increased growth rate over two generations. Our objectives were: 1) evaluate effects based overall physiological changes...
As part of our Asian seabass genome project, we are generating an inventory repeat elements in the and transcriptome. The karyotype showed a diploid number 2n = 24 chromosomes with variable B-chromosomes. transcriptome were searched for repetitive experimental bioinformatics tools. Six different types repeats constituting 8-14% characterized. Repetitive clustered pericentromeric heterochromatin all chromosomes, but some them preferentially accumulated pretelomeric regions several pairs have...
The Asian seabass (or barramundi; Lates calcarifer) is a marine teleost and popular food fish in Southeast Asia Australia. To date, comprehensive genome transcriptome sequence information has not been available for this species public repositories. Here, we report de novo assembly of the seabass. These data will be useful development molecular tools use aquaculture as well resource annotation. was obtained from sequences generated organs multiple individuals using three different...
Malabar snapper ( Lutjanus malabaricus ) is an economically important marine fish throughout the Indo‐Pacific, with emerging aquaculture industry. Although generic feeds are available for production, these not optimised this species. Understanding energy utilisation and balance can provide insight into suitable macronutrient profiles new species to a baseline future development. This study, therefore, evaluated effect of dietary composition (i.e., protein, fat, carbohydrate) on efficiencies...
Abstract The Asian seabass is an important marine food fish that has been cultured for several decades in Asia Pacific. However, the lack of a high quality reference genome hampered efforts to improve its selective breeding. A 3D BAC pool set generated this study was screened using 22 SSR markers located on linkage group 2 which contains growth-related QTL region. Seventy-two clones corresponding FPC contigs were sequenced by Illumina MiSeq technology. We co-assembled MiSeq-derived scaffolds...
Abstract Outbreaks of diseases in farmed fish remain a recurring problem despite the development vaccines and improved hygiene standards on aquaculture farms. One commonly observed bacterial disease tropical South-East Asian region is tenacibaculosis, which attributed to members Bacteroidetes genus Tenacibaculum , most notably T. maritimum . The impact tenacibaculosis microbiota remains poorly understood. In this study, we analysed different tissue types commercially reared seabass ( Lates...