A5051837759- Methane Hydrates and Related Phenomena
- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Geophysics and Gravity Measurements
- Astrophysics and Cosmic Phenomena
- Ionosphere and magnetosphere dynamics
- Superconducting Materials and Applications
- Seismology and Earthquake Studies
- Soil Moisture and Remote Sensing
- Solar and Space Plasma Dynamics
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Soil and Water Nutrient Dynamics
- Soil Carbon and Nitrogen Dynamics
- Isotope Analysis in Ecology
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Wastewater Treatment and Nitrogen Removal
- Marine and coastal ecosystems
- Soil and Unsaturated Flow
- Groundwater and Isotope Geochemistry
- Climate change and permafrost
- Environmental DNA in Biodiversity Studies
- Fish Ecology and Management Studies
- Bacteriophages and microbial interactions
Pacific Northwest National Laboratory
2015-2024
Battelle
2010-2018
Richland College
2018
Environmental transitions often result in resource mixtures that overcome limitations to microbial metabolism, resulting biogeochemical hotspots and moments. Riverine systems, where groundwater mixes with surface water (the hyporheic zone), are spatially complex temporally dynamic, making development of predictive models challenging. Spatial temporal variations zone communities a key, but understudied, component riverine function. Here, investigate the coupling among groundwater–surface...
The hyporheic corridor (HC) encompasses the river-groundwater continuum, where mixing of groundwater (GW) with river water (RW) in HC can stimulate biogeochemical activity. Here we propose a novel thermodynamic mechanism underlying this phenomenon and reveal broader impacts on dissolved organic carbon (DOC) microbial ecology. We show that thermodynamically favorable DOC accumulates GW despite lower concentration, RW contains less-favorable DOC, but at higher concentrations. This indicates is...
Subsurface groundwater-surface water mixing zones (hyporheic zones) have enhanced biogeochemical activity, but assembly processes governing subsurface microbiomes remain a critical uncertainty in understanding hyporheic biogeochemistry. To address this obstacle, we investigated (a) biogeographical patterns attached and waterborne across three hydrologically-connected, physicochemically-distinct (inland hyporheic, nearshore river); (b) that generated these patterns; (c) groups of organisms...
Spatial isolation of soil organic carbon (SOC) in different sized pores may be a mechanism by which otherwise labile (C) could protected soils. When water content increases, the hydrologic connectivity also allowing greater transport SOC and other resources from locations, to microbially colonized locations more favorable decomposition. The heterogeneous distribution specialized decomposers, C, throughout indicates that metabolism or persistence C compounds is highly dependent on...
Community assembly processes generate shifts in species abundances that influence ecosystem cycling of carbon and nutrients, yet our understanding remains largely separate from ecosystem-level functioning. Here, we investigate relationships between changes microbial metabolism across space time hyporheic communities. We pair sampling two habitat types (i.e., attached planktonic) through seasonal sub-hourly hydrologic fluctuation with null modeling temporally-explicit multivariate statistics....
Abstract Climate change is intensifying the global water cycle, with increased frequency of drought and flood. Water an important driver soil carbon dynamics, it crucial to understand how moisture disturbances will affect availability fluxes in soils. Here we investigate role substrate-microbe connectivity cycling under extreme conditions. We collected soils from Alaska, Florida, Washington USA, incubated them Drought Flood had a stronger effect on respiration, pore-water carbon, microbial...
Recent evidence has linked the gut microbiome to host behavior via gut-brain axis [1-3]; however, underlying mechanisms remain unexplored. Here, we determined links between genetics, and memory using genetically defined Collaborative Cross (CC) mouse cohort, complemented with metabolomic analyses in conventional germ-free (GF) mice.A genome-wide association analysis (GWAS) identified 715 of 76,080 single-nucleotide polymorphisms (SNPs) that were significantly associated short-term passive...
Soil microorganisms carry out key processes for life on our planet, including cycling of carbon and other nutrients supporting growth plants. However, there is poor molecular-level understanding their functional roles in ecosystem stability responses to environmental perturbations. This knowledge gap largely due the difficulty culturing majority soil microbes. Thus, use culture-independent approaches, such as metagenomics, promises direct assessment potential microbiomes. is, however, a...
Understanding drivers of permafrost microbial community composition is critical for understanding microbiology and predicting ecosystem responses to thaw. We hypothesize that communities are shaped by physical constraints imposed prolonged freezing, exhibit spatial distributions reflect dispersal limitation selective pressures associated with these constraints. To test this, we characterized patterns environmental variation in across an Alaskan boreal forest landscape. used null modeling...
Abstract Environmental metabolomes are fundamentally coupled to microbially-linked biogeochemical processes within ecosystems. However, significant gaps exist in our understanding of their spatiotemporal organization, limiting ability uncover transferrable principles and predict ecosystem function. We propose that a theoretical paradigm, which integrates concepts from metacommunity ecology, is necessary reveal underlying mechanisms governing metabolomes. call this synthesis between ecology...
The soil microbiome carries out important ecosystem functions, but interactions between microbial communities have been difficult to study due the high diversity and complexity of habitat. In this study, we successfully obtained stable consortia with reduced that contained species found in original source soil. These methods used obtain them can be a valuable resource for exploration specific mechanisms underlying community ecology. results also provide new experimental context better inform...
Climate change is causing shifts in precipitation patterns the central grasslands of United States, with largely unknown consequences on collective physiological responses soil microbial community, i.e., metaphenome. Here, we used an untargeted omics approach to determine community's metaphenomic response moisture and define specific metabolic signatures response. Specifically, aimed develop technical approaches mapping framework necessary for future systematic ecological studies. We...
Commensal microorganisms in the mammalian gut play important roles host health and physiology, but a central challenge remains achieving detailed mechanistic understanding of specific microbial contributions to biochemistry. New function-based approaches are needed that analyze function at molecular level by coupling detection measurements situ biochemical activity with identification responsible microbes enzymes. We developed platform employing β-glucuronidase selective activity-based...
River corridor metabolomes reflect organic matter (OM) processing that drives aquatic biogeochemical cycles. Recent work highlights the power of ultrahigh-resolution mass spectrometry for understanding metabolome composition and river metabolism. However, there have been no studies on global chemogeography surface water sediment using techniques. Here, we describe a community science effort from Worldwide Hydrobiogeochemistry Observation Network Dynamic Systems (WHONDRS) consortium to...
Soil viruses are abundant, but the influence of environment and climate on soil remains poorly understood. Here, we addressed this gap by comparing diversity, abundance, lifestyle, metabolic potential DNA in three grassland soils with historical differences average annual precipitation, low eastern Washington (WA), high Iowa (IA), intermediate Kansas (KS).
The effects of climate change on soil organic matter—its structure, microbial community, carbon storage, and respiration response—remain uncertain widely debated. In addition, the changes ecosystem structure function are often modulated or delayed, meaning that short-term experiments not sufficient to characterize responses. This study capitalized a long-term reciprocal transplant experiment examine response dryland soils change. two sites were separated by 500 m elevation same mountain...
Background Many human microbial infectious diseases including dental caries are polymicrobial in nature. How these complex multi-species communities evolve from a healthy to diseased state is not well understood. Although many health- or disease-associated oral bacteria have been characterized vitro, their physiology within the microbiome difficult determine with current approaches. In addition, about half of species remain uncultivated date little known besides 16S rRNA sequence. Lacking...
The soil microbiome represents one of the most complex microbial communities on planet, encompassing thousands taxa and metabolic pathways, rendering holistic analyses computationally intensive difficult. Here, we developed an alternative approach in which was broken into components ("functional modules"), based capacities, for individual characterization. We hypothesized that reproducible, low-complexity represent functional modules could be obtained through targeted enrichments that,...