A5088912262- Seismic Waves and Analysis
- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Microbial Community Ecology and Physiology
- Soil and Water Nutrient Dynamics
- Metabolomics and Mass Spectrometry Studies
- Marine and coastal ecosystems
- Biofuel production and bioconversion
- Isotope Analysis in Ecology
- Genomics and Phylogenetic Studies
- Fish Ecology and Management Studies
- Smart Materials for Construction
- Protist diversity and phylogeny
- Gut microbiota and health
- Advanced Chemical Sensor Technologies
- Agriculture, Soil, Plant Science
- Soil Carbon and Nitrogen Dynamics
- Groundwater and Isotope Geochemistry
- Geophysics and Gravity Measurements
- Bacteriophages and microbial interactions
- Wastewater Treatment and Nitrogen Removal
- Sustainability and Ecological Systems Analysis
- Hydrology and Watershed Management Studies
- Seismology and Earthquake Studies
- Mine drainage and remediation techniques
Pacific Northwest National Laboratory
2018-2025
Hiram College
2021-2023
Environmental Molecular Sciences Laboratory
2022-2023
Battelle
2021
The Ohio State University
2014-2019
Environmental transitions often result in resource mixtures that overcome limitations to microbial metabolism, resulting biogeochemical hotspots and moments. Riverine systems, where groundwater mixes with surface water (the hyporheic zone), are spatially complex temporally dynamic, making development of predictive models challenging. Spatial temporal variations zone communities a key, but understudied, component riverine function. Here, investigate the coupling among groundwater–surface...
The Candidate Phyla Radiation (CPR) is a recently described expansion of the tree life that represents more than 15% all bacterial diversity and potentially contains over 70 different phyla. Despite this broad phylogenetic variation, these microorganisms appear to feature little functional diversity, with members generally characterized as obligate fermenters. Additionally, much data describing CPR phyla has been generated from limited number environments, constraining our knowledge their...
Three-dimensional variably saturated flow and multicomponent biogeochemical reactive transport modeling, based on published newly generated data, is used to better understand the interplay of hydrology, geochemistry, biology controlling cycling carbon, nitrogen, oxygen, iron, sulfur, uranium in a shallow floodplain. In this system, aerobic respiration generally maintains anoxic groundwater below an oxic vadose zone until seasonal snowmelt-driven water table peaking transports dissolved...
Microorganisms drive high rates of methanogenesis and carbon mineralization in wetland ecosystems. These signals are especially pronounced the Prairie Pothole Region North America, tenth largest ecosystem world. Sulfate reduction up to 22 μmol cm−3 day−1 have been measured these sediments, as well methane fluxes 160 mg m−2 h−1—some highest emissions ever American wetlands. While pore waters from PPR wetlands characterized by concentrations sulfur species dissolved organic carbon, constraints...
Abstract Environmental metabolomes are fundamentally coupled to microbially-linked biogeochemical processes within ecosystems. However, significant gaps exist in our understanding of their spatiotemporal organization, limiting ability uncover transferrable principles and predict ecosystem function. We propose that a theoretical paradigm, which integrates concepts from metacommunity ecology, is necessary reveal underlying mechanisms governing metabolomes. call this synthesis between ecology...
Microbial ecological processes are frequently studied in the presence of perturbations rather than undisturbed environments, despite relatively stable conditions dominating many microbial habitats. To examine influencing community structuring absence strong external perturbations, three unperturbed aquifers Ohio (Greene, Licking, and Athens) were sampled over 2 years analyzed using geochemical measurements, 16S rRNA gene sequencing, modeling. Redox ranging from highly reducing to more...
Xanthohumol (XN), a polyphenol found in the hop plant (Humulus lupulus), has antioxidant, anti-inflammatory, prebiotic, and anti-hyperlipidemic activity. Preclinical evidence suggests gut microbiome is essential mediating these bioactivities; however, relatively little known about XN's impact on human microbiota vivo. We conducted randomized, triple-blinded, placebo-controlled clinical trial (ClinicalTrials.gov NCT03735420) to determine safety tolerability of XN healthy adults. Thirty...
Abstract Riverbed microbial communities play an oversized role in many watershed ecosystem functions, including the processing of organic carbon, cycling nitrogen, and alterations to metal mobility. The structure activity assemblages depend part on geochemical conditions set by river‐groundwater exchange or hyporheic exchange. To assess how seasonal changes mixing affect these populations a snowmelt‐dominated fluvial system, vertical sediment pore water profiles were sampled at three time...
River corridor metabolomes reflect organic matter (OM) processing that drives aquatic biogeochemical cycles. Recent work highlights the power of ultrahigh-resolution mass spectrometry for understanding metabolome composition and river metabolism. However, there have been no studies on global chemogeography surface water sediment using techniques. Here, we describe a community science effort from Worldwide Hydrobiogeochemistry Observation Network Dynamic Systems (WHONDRS) consortium to...
Abstract The primary aim of this study is to broadly evaluate the relationship between cursoriality (i.e. anatomical and physiological specialization for running) limb bone morphology in lagomorphs. Relative most previous studies cursoriality, our focus on a size‐restricted, taxonomically narrow group mammals permits us degree which ‘cursorial specialization’ affects locomotor anatomy independently broader allometric phylogenetic trends that might obscure such relationship. We collected...
Abstract Warmer and drier climate has contributed to increased occurrence of large, high severity wildfires in the Pacific Northwest, drawing concerns for water quality ecosystem recovery. While nutrient fluxes generally increase post‐fire, composition organic matter (OM) transported streams immediately following a fire is poorly constrained, yet can play an integral role downstream biogeochemistry. Here, we quantified spatiotemporal patterns dissolved OM (DOM) chemistry five burned by...
Soil microorganisms provide key ecological functions that often rely on metabolic interactions between individual populations of the soil microbiome. To better understand these and community processes, we used chitin, a major carbon nitrogen source in soil, as test substrate to investigate microbial during its decomposition. Chitin was applied model consortium developed, "model consortium-2" (MSC-2), consisting eight members diverse phyla including both chitin degraders nondegraders. A...
Abstract Background Microbiomes contribute to multiple ecosystem services by transforming organic matter in the soil. Extreme shifts environment, such as drying-rewetting cycles during drought, can impact microbial metabolism of altering physiology and function. These physiological responses are mediated part lipids that responsible for regulating interactions between cells environment. Despite this critical role response stress, little is known about metabolites soil or how they influence...
Disturbances cause rapid changes to forests, with different disturbance types and severities creating unique ecosystem trajectories that can impact the underlying soil microbiome. Pile burning-the combustion of logging residue on forest floor-is a common fuel reduction practice have impacts soils analogous those following high-severity wildfire. Further, pile burning clear-cut harvesting create persistent openings dominated by nonwoody plants surrounded dense regenerating conifer forest. A...
Two factors that are well-known to influence soil microbiomes the depth of as well level moisture. Previous works have demonstrated climate change will increase incidence drought in soils, but it is unknown how fluctuations moisture availability affect microbiome composition and functioning down profile. Here, we investigated wheatgrass rhizosphere a single common field setting under four different levels irrigation (100%, 75%, 50%, 25%) three depths (0-5 cm, 5-15 15-25 cm from surface). We...
Abstract. River corridors are fundamental components of the Earth system, and their biogeochemistry can be heavily influenced by processes in subsurface zones immediately below riverbed, referred to as hyporheic zone. Within zone, organic matter (OM) fuels microbial respiration, OM chemistry influences aerobic anaerobic biogeochemical processes. The link between respiration has been hypothesized mediated molecular diversity, whereby is predicted decrease with increasing diversity. Here we...
Dissolved organic matter (DOM) is vital to ecosystem functions, influencing nutrient cycles and water quality. Understanding the processes driving DOM chemistry variation remains a challenge. By examining these through community ecology perspective, we aim understand balance between stochastic forces (e.g., random mixing of DOM) deterministic systematic loss certain types molecules) shaping chemistry. Previous research on influences over applied null models aquatic environments subsurface...
Shallow riparian aquifers represent hotspots of biogeochemical activity in the arid western US. While these environments provide extensive ecosystem services, little is known how natural environmental perturbations influence subsurface microbial communities and associated processes. Over a six-month period we tracked annual snowmelt-driven incursion groundwater into vadose zone an aquifer adjacent to Colorado River, leading increased dissolved oxygen (DO) concentrations normally suboxic...
The ecological drivers that concurrently act upon both a virus and its host drive community assembly are poorly understood despite known interactions between viral populations their microbial hosts. Hydraulically fractured shale environments provide access to closed ecosystem in the deep subsurface where constrained processes can be examined. Here, we used metagenomic analyses of time-resolved-produced fluid samples from two wells Appalachian Basin track dynamics investigate processes....
Abstract Trace organic compounds (TrOCs) enter rivers with discharge of treated wastewater. These effluents can contain high loads dissolved matter (DOM). In a 48 h field study, we investigated changes in molecular composition seven DOM compound classes (FTICR-MS) and attenuation 17 polar TrOCs small urban stream receiving Correlations between were used to identify simultaneous surface water the hyporheic zone. Changes TrOC concentrations ranged decrease 29.2% for methylbenzotriazole an...
Abstract. Conceptual frameworks linking microbial community membership, properties, and processes with the environment emergent function have been proposed but remain untested. Here we refine test a recent conceptual framework using hyporheic zone sediments exposed to wetting–drying transitions. Our refined includes relationships between cumulative properties of (e.g., assembly biogeochemical rates), environmental features organic matter thermodynamics), ecosystem function. primary aim was...
A large amount of dissolved organic matter (DOM) is transported to the ocean from terrestrial inputs each year (~0.95 Pg C per year) and undergoes a series abiotic biotic reactions, causing significant release CO 2 . Combined, these reactions result in variable DOM characteristics (e.g., nominal oxidation state carbon, double-bond equivalents, chemodiversity) which have demonstrated impacts on biogeochemistry ecosystem function. Despite this importance, however, comparatively few studies...
Abstract. Biochemical transformations of organic matter (OM) are a primary driver river corridor biogeochemistry, thereby modulating ecosystem processes at local to global scales. OM driven by diverse biotic and abiotic processes, but we lack knowledge how the diversity those varies across corridors surface subsurface components corridors. To fill this gap quantified number putative molecules using ultra-high-resolution mass spectrometry. The unique is used here as proxy for biochemical...