A5053820774- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Atmospheric and Environmental Gas Dynamics
- Wastewater Treatment and Nitrogen Removal
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Corrosion Behavior and Inhibition
- Building materials and conservation
- Hydrocarbon exploration and reservoir analysis
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- CO2 Sequestration and Geologic Interactions
- Anaerobic Digestion and Biogas Production
- Food Safety and Hygiene
- Marine and coastal ecosystems
- Constructed Wetlands for Wastewater Treatment
- Listeria monocytogenes in Food Safety
- Metal Extraction and Bioleaching
- Enterobacteriaceae and Cronobacter Research
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Medical Device Sterilization and Disinfection
- Protist diversity and phylogeny
Radboud University Nijmegen
2019-2025
University of Amsterdam
2024-2025
Institute for Biodiversity
2025
University of Groningen
2023-2024
Radboud Institute for Molecular Life Sciences
2022-2024
Radboud University Medical Center
2022-2024
Health First
2021
Instituto Nacional de Investigação Agrária e Veterinária
2003-2021
The Ohio State University
2015-2018
Universidade Federal do Rio Grande do Sul
2013-2014
Rising seawater temperature and ocean acidification threaten the survival of coral reefs. The relationship between physiology its microbiome may reveal why some corals are more resilient to these global change conditions. Here, we conducted first experiment simultaneously investigate changes in response dual stress elevated expected by end this century. Two species corals, Acropora millepora containing thermally sensitive endosymbiont C21a Turbinaria reniformis tolerant Symbiodinium trenchi,...
Inland waters are increasingly recognized as critical sites of methane emissions to the atmosphere, but biogeochemical reactions driving such fluxes less well understood. The Prairie Pothole Region (PPR) North America is one largest wetland complexes in world, containing millions small, shallow wetlands. sediment pore PPR wetlands contain some highest concentrations dissolved organic carbon (DOC) and sulfur species ever recorded terrestrial aquatic environments. Using a suite geochemical...
Microorganisms drive high rates of methanogenesis and carbon mineralization in wetland ecosystems. These signals are especially pronounced the Prairie Pothole Region North America, tenth largest ecosystem world. Sulfate reduction up to 22 μmol cm−3 day−1 have been measured these sediments, as well methane fluxes 160 mg m−2 h−1—some highest emissions ever American wetlands. While pore waters from PPR wetlands characterized by concentrations sulfur species dissolved organic carbon, constraints...
Methane (CH 4 ) is a key greenhouse gas. Coastal areas account for major proportion of marine CH emissions. Eutrophication and associated bottom water hypoxia enhance production in coastal sediments. Here, we assess the fate produced sediments at site seasonally anoxic eutrophic basin (Scharendijke, Lake Grevelingen, Netherlands) spring (March) late summer (September) 2020. Removal sediment through anaerobic oxidation with sulfate <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Coastal zones account for 75% of marine methane emissions, despite covering only 15% the ocean surface area. In these ecosystems, tight balance between production and oxidation in sediments prevents most from escaping into seawater. However, anthropogenic activities could disrupt this balance, leading to an increased escape coastal sediments. To quantify unravel potential mechanisms underlying disruption, we used a suite biogeochemical microbiological analyses investigate impact...
ABSTRACT The hemolytic ability, the presence of cyl genes, and diagnostic accuracy cytolysin molecular detection were investigated in genus Enterococcus by using 164 strains from 20 different species (26 reference strains, 42 clinical isolates human veterinary origin, 96 ewe cheese milk). Hemolysis was assayed with sheep horse erythrocytes under aerobic or anaerobic conditions. Screening genes ( cylL L , S cylM cylB cylA ) performed new specific primers assay beta-hemolysis used as “gold...
The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples 55) Porto Alegre, Brazil, collected 2006 2009. In addition, ability form biofilm vitro on polystyrene β-haemolytic gelatinase activities were determined. Clinical strains presented higher prevalence aggregation substance (agg), enterococcal surface protein (esp) cytolysin (cylA) genes when...
Microorganisms are main drivers of the sulfur, nitrogen and carbon biogeochemical cycles. These elemental cycles interconnected by activity different guilds in sediments or wastewater treatment systems. Here, we investigated a nitrate-reducing microbial community laboratory-scale bioreactor model that closely mimicked estuary brackish sediment conditions. The simultaneously consumed sulfide, methane ammonium at expense nitrate. Ammonium oxidation occurred solely anammox bacteria identified...
Bacteria in the order 'Candidatus Brocadiales' within phylum Planctomycetes (Planctomycetota) have remarkable ability to perform anaerobic ammonium oxidation (anammox). Two families of anammox bacteria with different biogeographical distributions been reported, marine Ca. Scalinduaceae and freshwater Brocadiaceae. Here we report evidence three new species a novel genus family bacteria, which were discovered biofilms subsea road tunnel under fjord Norway. In this particular ecosystem,...
The long-term fate of plastics in the ocean and their interactions with marine microorganisms remain poorly understood. In particular, role sinking plastic particles as a transport vector for surface microbes towards deep sea has not been investigated. Here, we present first data on composition microbial communities floating suspended recovered from to bathypelagic water column (0-2000 m depth) North Pacific Subtropical Gyre. Microbial community differed that afloat at surface. However, both...
The potential and drivers of microbial methane removal in the water column seasonally stratified coastal ecosystems importance methanotrophic community composition for ecosystem functioning are not well explored. Here, we combined depth profiles oxygen with 16S rRNA gene amplicon sequencing, metagenomics oxidation rates at discrete depths a marine system (Lake Grevelingen, Netherlands). Three sequence variants (ASVs) belonging to different genera aerobic Methylomonadaceae corresponding three...
The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface exposed to oxygen. Concrete biodeterioration and steel corrosion have been linked growth of iron- manganese-oxidizing biofilms areas saline water seepage. Surprisingly, previous 16S rRNA gene surveys biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. This study aimed identify genomes metabolic potential for novel...
Methane is a powerful greenhouse gas that produced in large quantities marine sediments. Microbially mediated oxidation of methane sediments, when balance with production, prevents the release to overlying water. Here, we present gene-based reactive transport model includes both microbial and geochemical dynamics use it investigate whether rate growth oxidizers sediments impacts efficiency filter. We focus on iron- methane-rich coastal and, model, show at our site, up 10% all removed...
The ability of antibiotic resistant E. faecalis and faecium isolated from food to form biofilm at different temperatures in the absence or presence 0.75% glucose was evaluated. A synergistic effect on 10 °C, 28 37 °C 45 observed for faecium.
Microbial biofilm formation can contribute to the accelerated deterioration of steel-reinforced concrete structures and significantly impact their service life, making it critical understand diversity community prevailing processes in these habitats. Here, we analyzed 16S rRNA gene amplicon metagenomics sequencing data study abundance nitrifiers within biofilms on surface Oslofjord subsea road tunnel Norway. We showed that varied greatly time space, with a mean 24.7±15% but wide range...
Coagulase-positive staphylococci (CPS) cause staphylococcal food poisoning. Recently, these bacteria have received increasing attention due to their potential role in the dissemination of antibiotic resistance markers. The present study aimed evaluate coagulase-positive counts, species distribution, enterotoxin genes prevalence, and profile CPS isolated from natura chicken meat. Fifteen frozen 15 chilled industrialized, uncooked parts or entire carcasses were used. Staphylococcal counts...
Microbial methane oxidation is a major biofilter preventing larger emissions of this powerful greenhouse gas from marine coastal areas into the atmosphere. In these zones, various electron acceptors such as sulfate, metal oxides, nitrate, or oxygen can be used. However, key microbial players and mechanisms are poorly understood. study, we inoculated bioreactor with methane- iron-rich sediments Bothnian Sea to investigate iron cycling under low concentrations. Using metagenomics, investigated...
The high-quality draft genome of "Candidatus Methanoperedens sp." strain BLZ2, a nitrate-reducing archaeon anaerobically oxidizing methane, is presented. was obtained from an enrichment culture and measures 3.74 Mb. It harbors two nitrate reductase gene clusters, ammonium-forming nitrite reductase, the complete reverse methanogenesis pathway. Methane that escapes to atmosphere acts as potent greenhouse gas. Global methane emissions are mitigated by methanotrophs, which oxidize CO2 spp."...
Understanding patterns of organic matter degradation in wetlands is essential for identifying the substrates and mechanisms supporting greenhouse gas production emissions from wetlands, main natural source methane atmosphere. Alcohols are common fermentation products but poorly studied as key intermediates wetlands. By investigating genes, pathways, microorganisms potentially accounting high concentrations ethanol isopropanol measured Prairie Pothole wetland sediments, this work advanced our...
Microbial communities are key drivers of carbon, sulfur, and nitrogen cycling in coastal ecosystems, where they subjected to dynamic shifts substrate availability exposure toxic compounds. However, how these affect microbial interactions function is poorly understood. Unraveling such community responses understand their environmental distribution resilience under current future disturbances. Here, we used metagenomics metatranscriptomics investigate structure transcriptional prolonged...
The aim of this study was to evaluate the species distribution, antibiotic-resistance profile and presence enterotoxin (SE) genes in staphylococci isolated from Dilúvio stream South Brazil. Eighty-eight were identified, 93.18% identified as coagulase-negative (CNS) 6.82% coagulase-positive (CPS). Fourteen Staphylococcus detected most frequently cohnii (30.48%) S. haemolyticus (21.95%). Resistance erythromycin verified 37.50% strains, followed by 27.27% penicillin, 12.50% clindamycin, 6.81%...
Summary Methoxylated aromatic compounds (MACs) are important components of lignin found in significant amounts the subsurface. Recently, methanogenic archaeon Methermicoccus shengliensis was shown to be able use a variety MACs during methoxydotrophic growth. After molecular survey, we that hyperthermophilic non‐methanogenic Archaeoglobus fulgidus also encodes genes for bacterial‐like demethoxylation system. In this study, performed growth and metabolite analysis, used transcriptomics...
Abstract Coastal zones account for significant global marine methane emissions to the atmosphere. In coastal ecosystems, tight balance between microbial production and oxidation in sediments prevents most from escaping water column. Anthropogenic activities, causing eutrophication bottom deoxygenation, could disrupt this cycle lead increased release sediments. Here, we combined microbiological biogeochemical analyses of three sites along a redox gradient (oxic-hypoxic-euxinic) eutrophic...