A5000611930- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Trace Elements in Health
- Infective Endocarditis Diagnosis and Management
- Enzyme Catalysis and Immobilization
- Mycobacterium research and diagnosis
- Identification and Quantification in Food
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- Crystallography and molecular interactions
- Microbial Metabolism and Applications
- Iron Metabolism and Disorders
- Corrosion Behavior and Inhibition
- Microbial Inactivation Methods
- Organometallic Compounds Synthesis and Characterization
- Antimicrobial agents and applications
- Growth and nutrition in plants
National Institute of Health Dr. Ricardo Jorge
2024
Universidade Nova de Lisboa
2003-2018
Escola Secundária Quinta do Marquês
2009-2018
Instituto de Biologia Experimental e Tecnológica
1999-2018
GenIbet (Portugal)
2013
Instituto Português Da Qualidade
2012
University of Lisbon
2011
Instituto de Tecnología Química
1999-2003
Polylysogeny is frequently considered to be the result of an adaptive evolutionary process in which prophages confer fitness and/or virulence factors, thus making them important for evolution both bacterial populations and infectious diseases. The Enterococcus faecalis V583 isolate belongs high-risk clonal complex 2 that particularly well adapted hospital environment. Its genome carries 7 prophage-like elements (V583-pp1 -pp7), one ubiquitous species. In this study, we investigated activity...
ABSTRACT The hemolytic ability, the presence of cyl genes, and diagnostic accuracy cytolysin molecular detection were investigated in genus Enterococcus by using 164 strains from 20 different species (26 reference strains, 42 clinical isolates human veterinary origin, 96 ewe cheese milk). Hemolysis was assayed with sheep horse erythrocytes under aerobic or anaerobic conditions. Screening genes ( cylL L , S cylM cylB cylA ) performed new specific primers assay beta-hemolysis used as “gold...
β-Cyclodextrin (βCD) inclusion compounds of chloramphenicol in the solid-state were prepared via two different procedures, co-crystallisation from aqueous solution and solvent-free method co-grinding. Chloramphenicol into permethylated βCD (TRIMEB) was also performed by co-dissolution ethanol. The procedure best for preparing chloramphenicol·βCD 1 : stoichiometry high purity, while with co-grinding treatment same starting proportion, a complete not achieved. Microcrystals compound presented...
Despite the existence of various virulence factors in Enterococcus genus, enterococcal is still a debated issue. A main consideration detection same genes strains isolated from nosocomial or community-acquired infections, and food products. The goal this study was to evaluate roles two well-characterized factors, Fsr gelatinase, potential faecalis strains. Virulence unrelated isolates, including dairy carrying fsr gelE operons, compared Galleria mellonella insect model. E. were able kill...
Mechanisms that enable Enterococcus to cope with different environmental stresses and their contribution the switch from commensalism pathogenicity of this organism are still poorly understood. Maintenance intracellular homeostasis metal ions is crucial for survival these bacteria. In particular Zn2+, Mn2+ Cu2+ very important as they co-factors many enzymes, involved in oxidative stress defense have a role immune system host. Their concentrations inside human body vary hugely, which makes it...
ObjectivesTo investigate the role of a putative small multidrug resistance transporter, annotated in Enterococcus faecalis V583 genome as EFA0010 (we will refer to this gene qacZ), decreased susceptibility biocides.
Enterococcus faecalis V583 is a vancomycin-resistant clinical isolate which belongs to the hospital-adapted clade, CC2. This strain harbours several factors that have been associated with virulence, including fsr quorum-sensing regulatory system known control expression of GelE and SprE proteases. To discriminate between genes directly regulated by Fsr, those indirectly as result protease or activity, we compared gene in isogenic mutants variously defective either Fsr quorum sensing...
Metal ions, in particular manganese, are important modulators of bacterial pathogenicity. However, little is known about the role manganese-dependent proteins nosocomial pathogen Enterococcus faecalis, a major cause endocarditis. The present study demonstrates that DtxR/MntR family metalloregulator EfaR E. faecalis controls expression several its regulon members way. We also show efaR inactivation impairs ability to form biofilms, survive inside macrophages, and tolerate oxidative stress....
The concomitant presence of a complete fsr quorum-sensing system and gelE-sprE operons in Enterococcus faecalis is known to be essential for the detection gelatinase activity. However, there are reports absence activity despite gelE loci. In order understand this incongruence between genotype phenotype we sequenced loci E. LN68 strain, which was previously found carry both but lack Of 59 nucleotide differences detected compared with gelatinase-positive V583 nonsense mutation (a premature...
Enterococci isolated from Portuguese dairy products (milk and cheese) clinical settings (hospitals veterinary clinics), together with reference strains the genus Enterococcus, were screened for low- high-level gentamicin resistance using standard disc diffusion method (10 120 µg discs). MICs also determined both macrodilution Etest. Four genes [aac(6′)-Ie-aph(2″)-Ia, aph(2″)-Ib, aph(2″)-Ic aph(2″)-Id] responsible high- mid-level sought PCR. Although enterococci generally are regarded as...
ABSTRACT Cheese produced from raw ewes’ milk and chouriço , a Portuguese dry fermented sausage, are still in traditional way certain regions of Portugal by relying on colonization microbial populations associated with the materials, equipment, local environments. For purpose describing product origins types these foods, metabolic phenotypes can be used as descriptors well to determine presence compounds organoleptic value. The application artificial neural networks profiles bacterial...
Two Enterococcus faecalis genes encoding the P-type ATPase EF1400 and putative SapB protein EF0759 were previously shown to be strongly upregulated in presence of high concentrations zinc. In present work, we showed that a Zn(2+)-responsive DNA-binding motif (zim) is promoter regions these genes. Both proteins further studied with respect their involvement zinc homeostasis invasion host. contributed intramacrophage survival by an as-yet unknown mechanism(s). EF1400, here renamed ZntAEf,...