A5061751596- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Astrophysics and Cosmic Phenomena
- Gut microbiota and health
- Marine and coastal ecosystems
- Gene Regulatory Network Analysis
- Methane Hydrates and Related Phenomena
- Microbial Fuel Cells and Bioremediation
- Bioinformatics and Genomic Networks
- Photosynthetic Processes and Mechanisms
- Evolutionary Game Theory and Cooperation
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Regional Development and Policy
- Mass Spectrometry Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Anaerobic Digestion and Biogas Production
- Bacterial biofilms and quorum sensing
- Economic Growth and Productivity
- CO2 Sequestration and Geologic Interactions
UiT The Arctic University of Norway
2018-2025
Pacific Northwest National Laboratory
2014-2023
Centre for Arctic Gas Hydrate, Environment and Climate
2018-2019
Washington State University
2016-2019
Battelle
2018
Montana State University
2010-2016
Purple Mountain Observatory
2007
Much research has been invested into engineering microorganisms to perform desired biotransformations; nonetheless, these efforts frequently fall short of expected results due the unforeseen effects biofeedback regulation and functional incompatibility. In nature, metabolic function is compartmentalized diverse organisms assembled robust consortia, in which division labor thought lead increased community efficiency productivity. Here we consider whether how consortia can be designed...
Abstract Biofilms have been implicated in delayed wound healing, although the mechanisms by which biofilms impair healing are poorly understood. Many species of bacteria produce exotoxins and exoenzymes that may inhibit healing. In addition, oxygen consumption responding leukocytes, impede depleting is required for this study, microsensors to measure transects through vitro cultured biofilms, formed vivo within scabs from a diabetic (db/db) mouse model, ex human chronic specimens was used....
Metabolic network modeling of microbial communities provides an in-depth understanding community-wide metabolic and regulatory processes. Compared to single organism analyses, community is more complex because it needs account for interspecies interactions. To date, most approaches focus on reconstruction high-quality individual networks so that, when combined, they can predict behaviors as a result However, this conventional method becomes ineffective whose members are not well...
Bacterial biofilm formation is a complex developmental process involving cellular differentiation and the of intricate 3D structures. Here we demonstrate that exposure to ferric chloride triggers rugose by uropathogenic Escherichia coli strain UTI89 enteric bacteria Citrobacter koseri Salmonella enterica serovar typhimurium . Two unique separable populations emerge in iron-triggered, biofilms. Bacteria at air–biofilm interface express high levels regulator csgD , cellulose activator adrA...
The soil microbiome carries out important ecosystem functions, but interactions between microbial communities have been difficult to study due the high diversity and complexity of habitat. In this study, we successfully obtained stable consortia with reduced that contained species found in original source soil. These methods used obtain them can be a valuable resource for exploration specific mechanisms underlying community ecology. results also provide new experimental context better inform...
A new co-cultivation technology is presented that converts greenhouse gasses, CH4 and CO2, into microbial biomass. The methanotrophic bacterium, Methylomicrobium alcaliphilum 20z, was coupled to a cyanobacterium, Synechococcus PCC 7002 via oxygenic photosynthesis. system exhibited robust growth on diverse gas mixtures ranging from biogas those representative of natural feedstock. continuous processes developed synthetic feed achieved steady-state by imposing light O2 limitations the...
Harnessing the metabolic potential of photosynthetic microbes for next-generation biotechnology objectives requires detailed scientific understanding physiological constraints and regulatory controls affecting carbon partitioning between biomass, metabolite storage pools, bioproduct synthesis. We dissected cellular mechanisms underlying remarkable robustness euryhaline unicellular cyanobacterium Synechococcus sp. strain PCC 7002 (Synechococcus 7002) identify key that allow cyanobacteria to...
Rising atmospheric carbon concentrations affect global health, the economy, and overall quality of life. We are fast approaching climate tipping points that must be addressed, not only by reducing emissions but also through new innovation action toward capture for sequestration utilization (CCSU). In this perspective, we delineate next-generation biotechnologies CCSU supported engineering design principles derived from ecological processes inspired three major biomes (plant-soil, deep...
Broad-host-range synthetic biology is an emerging frontier that aims to expand our current engineerable domain of microbial hosts for biodesign applications. As more novel species are brought “model status,” biologists discovering identically engineered genetic circuits can exhibit different performances depending on the organism it operates within, observation referred as “chassis effect.” It remains a major challenge uncover which genome-encoded and biological determinants will underpin...
The diatom lipidome actively regulates photosynthesis and displays a high degree of plasticity in response to light environment, either directly as structural modifications thylakoid membranes protein–pigment complexes, or indirectly via photoprotection mechanisms that dissipate excess energy. This acclimation is crucial maintaining primary production marine systems, particularly polar environments, due the large temporal variations both intensity wavelength distributions downwelling solar...
The choice of organism to host a genetic circuit, the chassis, is often defaulted model organisms due their amenability. chassis-design space has therefore remained underexplored as an engineering variable. In this work, we explored design toggle switch through variations in nine ribosome binding site compositions and three contexts, creating 27 circuit variants. Characterization performance metrics terms output growth dynamics unveils spectrum profiles from our library. We find that changes...
This is a protocol for using bi-parental conjugation to transfer plasmid into non-model recipient bacteria from DAP auxotroph donor strain.
Cold-water corals in the Hola area off coast of Vesterålen (N. Norway), thrive on a substrate made methane-derived carbonate and are closely associated with microbial mats. High resolution seafloor imagery sediment samples collected during EMAN7 expedition June 2022 allowed us to reconstruct spatial relationships between methane seepage habitats gain insights into subsurface biogeochemical processes directly influencing benthic ecosystems. Here, we present fine-scale orthomosaics...
Abstract Cold seeps and cold‐water corals (CWCs) coexist on Northern Norway's continental shelf at the Hola trough between Lofoten Vesterålen. Here, cold release methane from seabed, yet none reaches sea surface. Instead, dissolves disperses in ocean where it is ultimately consumed by methane‐oxidizing microorganisms. These microorganisms metabolize carbon dioxide dissolved organic matter (DOM), which may impact biogeochemical habitat of CWCs close vicinity seeps. We investigated...
The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model systems can yield some general principles related to this question. In study we employed naturally occurring co-culture composed heterotrophic bacteria, Halomonas sp. HL-48 Marinobacter HL-58, ask two scientific questions: 1) how do the phenotypes co-existing respond partnership as compared axenic growth? 2)...
Arctic marine environments are experiencing rapid changes due to the polar amplification of global warming. These impact habitat cold-adapted microbial communities, which underpin biogeochemical cycles and food webs. We comparatively investigated differences in prokaryotic microeukaryotic taxa between summer surface water microbiomes sampled along a latitudinal transect from ice-free southern Barents Sea into sea-ice-covered Nansen Basin disentangle dominating community (ecological)...
Abstract Background Biofilms are ubiquitous. For instance, the majority of medical infections thought to involve biofilms. However even after decades investigation, in vivo efficacy many antimicrobial strategies is still debated suggesting there a need for better understanding biofilm tolerances. The current study's goal characterize robustness antibiotic tolerance medically and industrially relevant culturing perturbations. By definition, robust systems will return similar, predictable...
Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily chlorophyll (680 nm) or phycocyanin (630 as organism subjected four distinct mono- dichromatic regimes. During cultivation with light, growth rates displayed generally proportional total incident irradiance at values < 275 µmol m-2 s-1...
Biomineralized ferric oxide microbial mats are ubiquitous features on Earth, common in hot springs of Yellowstone National Park (YNP, WY, USA), and form due to direct interaction between physicochemical processes. The overall goal this study was determine the contribution different community members assembly succession acidic high-temperature Fe(III)-oxide mat ecosystems. Spatial temporal changes accretion abundance relevant were monitored over 70 days using sterile glass microscope slides...