A5100352749- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Forensic and Genetic Research
- Pulmonary Hypertension Research and Treatments
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Nitric Oxide and Endothelin Effects
- MicroRNA in disease regulation
- Biosensors and Analytical Detection
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Genetic Associations and Epidemiology
- Gold and Silver Nanoparticles Synthesis and Applications
- interferon and immune responses
- Paraquat toxicity studies and treatments
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- COVID-19 Clinical Research Studies
Shenzhen University Health Science Center
2023-2025
Nanjing Medical University
2016-2025
Wuxi People's Hospital
2010-2025
Xi'an Jiaotong University
2012-2025
South China Agricultural University
2016-2025
Washington University in St. Louis
2013-2025
Nanjing University
2013-2025
Xinqiao Hospital
2016-2025
Stanford University
2014-2025
Wenzhou Medical University
2019-2025
We have produced a draft sequence of the rice genome for most widely cultivated subspecies in China, Oryza sativa L. ssp. indica , by whole-genome shotgun sequencing. The was 466 megabases size, with an estimated 46,022 to 55,615 genes. Functional coverage assembled sequences 92.0%. About 42.2% exact 20-nucleotide oligomer repeats, and transposons were intergenic regions between Although 80.6% predicted Arabidopsis thaliana genes had homolog rice, only 49.4% A. . large proportion no...
Coronavirus disease 2019 (COVID-19) is a pandemic with no specific therapeutic agents and substantial mortality. It critical to find new treatments.To determine whether convalescent plasma transfusion may be beneficial in the treatment of critically ill patients severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.Case series 5 laboratory-confirmed COVID-19 distress (ARDS) who met following criteria: pneumonia rapid progression continuously high viral load despite antiviral...
Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative are still lacking; specifically realm appropriate statistical treatment. Confidence interval and significance considerations not explicit many current approaches. Based on standard curve method other useful methods, we present compare four approaches models data.In first approach, a multiple regression model was developed to derive DeltaDeltaCt from...
The bacterial and archaeal genomes that have been sequenced to date were chosen for sequencing based mainly on their physiology, which is fine but has resulted in a distinct phylogenetic bias. An alternative approach taken the Genomic Encyclopedia of Bacteria Archaea (GEBA) project, advocates choosing organism's position, with aim filling gaps along branches tree life. value this demonstrated by pilot study genome sequences 56 culturable species selected maximize coverage. Analysis provides...
The OrthoMCL database (http://orthomcl.cbil.upenn.edu) houses ortholog group predictions for 55 species, including 16 bacterial and 4 archaeal genomes representing phylogenetically diverse lineages, most currently available complete eukaryotic genomes: 24 unikonts (12 animals, 9 fungi, microsporidium, Dictyostelium, Entamoeba), plants/algae 7 apicomplexan parasites. software was used to cluster proteins based on sequence similarity, using an all-against-all BLAST search of each species'...
Our knowledge of Neanderthals is based on a limited number remains and artifacts from which we must make inferences about their biology, behavior, relationship to ourselves. Here, describe the characterization these extinct hominids new perspective, development Neanderthal metagenomic library its high-throughput sequencing analysis. Several lines evidence indicate that 65,250 base pairs hominid sequence so far identified in are origin, strongest being ascertainment identities between...
Mutations in the microtubule-associated protein tau, including P301L, are genetically coupled to hereditary frontotemporal dementia with parkinsonism linked chromosome 17. To determine whether P301L is associated fibril formation mice, we expressed longest human tau isoform, tau40, this mutation transgenic mice by using neuron-specific mouse Thy1.2 promoter. We obtained high expression of cortical and hippocampal neurons. Accumulated was hyperphosphorylated translocated from axonal...
As the emerging variants of SARS-CoV-2 continue to drive worldwide pandemic, there is a constant demand for vaccines that offer more effective and broad-spectrum protection. Here, we report circular RNA (circRNA) vaccine elicited potent neutralizing antibodies T cell responses by expressing trimeric RBD spike protein, providing robust protection against in both mice rhesus macaques. Notably, circRNA enabled higher durable antigen production than 1mΨ-modified mRNA proportion distinct...
Orthology detection is critically important for accurate functional annotation, and has been widely used to facilitate studies on comparative evolutionary genomics. Although various methods are now available, there no comprehensive analysis of performance, due the lack a genomic-scale ‘gold standard’ orthology dataset. Even in absence such datasets, comparison results from alternative methodologies contains useful information, as agreement enhances confidence disagreement indicates possible...
Genes in prokaryotic genomes are often arranged into clusters and co-transcribed polycistronic RNAs. Isolated examples of RNAs were also reported some higher eukaryotes but their presence was generally considered rare. Here we developed a long-read sequencing strategy to identify transcripts several mushroom forming fungal species including Plicaturopsis crispa, Phanerochaete chrysosporium, Trametes versicolor, Gloeophyllum trabeum. We found genome-wide prevalence transcription these...
Organisms of the third domain life, Archaea, share molecular characteristics both with Bacteria and Eukarya. These organisms attract scientific attention as research models for regulation evolution processes such transcription, translation, RNA processing. We have reconstructed primary transcriptome Sulfolobus solfataricus P2, one most widely studied model archaeal organisms. Analysis 625 million bases sequenced cDNAs yielded a single-base-pair resolution map transcription start sites operon...
Long noncoding RNAs (lncRNAs) have emerged as critical players in cancer progression, but their functions colorectal (CRC) metastasis not been systematically clarified.lncRNA expression profiles matched normal and CRC tissue were checked using microarray analysis. The biological roles of a novel lncRNA, namely RP11-138 J23.1 (RP11), development both vitro vivo. Its association with clinical progression was further analyzed.RP11 highly expressed tissues, its increased stage patients. RP11...
Studies on biological functions of N6-methyladenosine (m6A) modification in mRNA have sprung up recent years. We find m6A can positively regulate the glycolysis cancer cells. Specifically, m6A-sequencing and functional studies confirm that pyruvate dehydrogenase kinase 4 (PDK4) is involved regulated ATP generation. The modified 5'UTR PDK4 regulates its translation elongation stability via binding with YTHDF1/eEF-2 complex IGF2BP3, respectively. Targeted specific demethylation by dm6ACRISPR...
For the past decade, cancer genomic studies have focused on mutations leading to splice-site disruption, overlooking those having splice-creating potential. Here, we applied a bioinformatic tool, MiSplice, for large-scale discovery of splice-site-creating (SCMs) across 8,656 TCGA tumors. We report 1,964 originally mis-annotated clear evidence creating alternative splice junctions. TP53 and GATA3 26 18 SCMs, respectively, ATRX has 5 from lower-grade gliomas. Mutations in 11 genes, including...
Abstract Studies on biological functions of N6-methyladenosine (m6A) modification in mRNA have drawn significant attention recent years. Here we describe the construction and characterization a CRISPR–Cas13b-based tool for targeted demethylation specific mRNA. A fusion protein, named dm6ACRISPR, was created by linking catalytically inactive Type VI-B Cas13 enzyme from Prevotella sp. P5–125 (dPspCas13b) to m6A demethylase AlkB homolog 5 (ALKBH5). dm6ACRISPR specifically demethylates such as...
Abstract Sodium-glucose cotransporter 2 (SGLT2) inhibitors reduce cardiovascular mortality in patients with diabetes mellitus but the protective mechanism remains elusive. Here we demonstrated that SGLT2 inhibitor, Empagliflozin (EMPA), suppresses cardiomyocytes autosis (autophagic cell death) to confer cardioprotective effects. Using myocardial infarction (MI) mouse models and without mellitus, EMPA treatment significantly reduced infarct size, fibrosis, thereby leading improved cardiac...