A5045789336- Plant tissue culture and regeneration
- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Bioenergy crop production and management
- Biofuel production and bioconversion
- Insect Resistance and Genetics
- Methane Hydrates and Related Phenomena
- Geophysics and Gravity Measurements
- Ionosphere and magnetosphere dynamics
- Weed Control and Herbicide Applications
- Animal Genetics and Reproduction
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Pesticide and Herbicide Environmental Studies
- American Constitutional Law and Politics
- Forest Biomass Utilization and Management
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Electoral Systems and Political Participation
- Nematode management and characterization studies
University of Tennessee at Knoxville
2016-2025
Massachusetts Institute of Technology
1989-2025
Oak Ridge National Laboratory
2014-2024
Bioenergy Life Science (United States)
2010-2022
University of Tennessee Health Science Center
2022
Chelsea and Westminster Hospital NHS Foundation Trust
2022
Knoxville College
2014-2021
Moscow Institute of Thermal Technology
2013-2020
Plant (United States)
2008-2015
University of Saskatchewan
2015
Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative are still lacking; specifically realm appropriate statistical treatment. Confidence interval and significance considerations not explicit many current approaches. Based on standard curve method other useful methods, we present compare four approaches models data.In first approach, a multiple regression model was developed to derive DeltaDeltaCt from...
Summary Switchgrass ( Panicum virgatum L.) has been developed into a dedicated herbaceous bioenergy crop. Biomass yield is major target trait for genetic improvement of switchgrass. microRNAs have emerged as prominent class gene regulatory factors that the potential to improve complex traits such biomass yield. A miR156b precursor was overexpressed in The effects miR156 overexpression on SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes were revealed by microarray and quantitative RT‐PCR...
• The major obstacle for bioenergy production from switchgrass biomass is the low saccharification efficiency caused by cell wall recalcitrance. Saccharification negatively correlated with both lignin content and ester-linked p-coumarate: ferulate (p-CA : FA) ratio. In this study, we cloned functionally characterized an R2R3-MYB transcription factor evaluated its potential developing lignocellulosic feedstocks. PvMYB4 cDNAs were expressed in Escherichia coli, yeast, tobacco functional...
Unresolved questions about evolution of the large and diverse legume family include timing polyploidy (whole-genome duplication; WGDs) relative to origin major lineages within Fabaceae symbiotic nitrogen fixation. Previous work has established that a WGD affects most in Papilionoideae occurred sometime after divergence papilionoid mimosoid clades, but exact been unknown. The history also not for outside Papilionoideae. We investigated presence WGDs legumes by querying thousands phylogenetic...
The evolutionary significance of introgression has been discussed for decades. Questions about potential impacts transgene flow into wild and weedy populations brought renewed attention to the crop alleles those populations. In past two decades, field advanced with considerable descriptive, experimental, theoretical activity on dynamics gene its consequences. As illustrated by five case studies employing an array different approaches, occurred a wide species, sometimes without significant...
The phytohormone auxin regulates nearly all aspects of plant growth and development. Based on the current model in Arabidopsis thaliana, Auxin/indole-3-acetic acid (Aux/IAA) proteins repress auxin-inducible genes by inhibiting response transcription factors (ARFs). Experimental evidence suggests that heterodimerization between Aux/IAA ARF are related to their unique biological functions. objective this study was generate Aux/IAA-ARF protein-protein interaction map using full length sequences...
•Understanding fern (monilophyte) phylogeny and its evolutionary timescale is critical for broad investigations of the evolution land plants, providing point comparison necessary studying sister group, seed plants. Molecular phylogenetic have revolutionized our understanding phylogeny, however, to date, these studies relied almost exclusively on plastid data.•Here we take a curated phylogenomics approach infer first from multiple nuclear loci, by combining taxon sampling (73 ferns 12...
1. Introduction 2. Literacy and orality 3. Oral poetry 4. The coming of the alphabet: literacy oral communication in archaic Greece 5. Beyond rationalist view writing: between 'literate' 'oral' 6. Orality, performance, memorial 7. state: profusion writing Epilogue: Roman world Bibliographical essay Bibliography.
Abstract Somatic embryos of Jack, a Glycine max (L.) Merrill cultivar, were transformed using microprojectile bombardment with synthetic Bacillus thuringiensis insecticidal crystal protein gene (Bt cryIAc) driven by the 35S promoter and linked to HPH gene. Approximately 10 g tissue was bombarded, three transgenic lines selected on hygromycin-containing media converted into plants. The recovered contained gene, but Bt lost in one line. plasmid rearranged second line, third line had two...
Abstract A method for estimating and comparing population genetic variation using random amplified polymorphic DNA (RAPD) profiling is presented. An analysis of molecular variance (AMOVA) extended to accomodate phenotypic data in diploid populations Hardy‐Weinberg equilibrium or with an assumed degree selfing. We present a two step strategy: 1) Estimate RAPD site frequencies without preliminary assumptions on the unknown structure, then perform significance testing substructuring. 2) If...
Abstract Background As a major component of plant cell wall, lignin plays important roles in mechanical support, water transport, and stress responses. the main cause for recalcitrance modification has been task bioenergy feedstock improvement. The study evolution function biosynthesis genes thus two-fold implications. First, pathway provides an excellent model to coordinative biochemical plants. Second, understanding will guide us develop better strategies Results We analyzed from fourteen...
The statistical treatment for hypothesis testing using real-time PCR data is a challenge quantification of gene expression. One has to consider two key factors in precise analysis data: well-defined model and the integration amplification efficiency (AE) into model. Previous publications often fall short integrating AE Novel, user-friendly, universal AE-integrated methods were developed with four goals. First, we addressed definition AE, introduced concept efficiency-adjusted Delta Ct,...
Abstract Background Arsenic is toxic to plants and a common environmental pollutant. There strong chemical similarity between arsenate [As (V)] phosphate (Pi). Whole genome oligonucleotide microarrays were employed investigate the transcriptional responses of Arabidopsis thaliana As (V) stress. Results Antioxidant-related genes (i.e. coding for superoxide dismutases peroxidases) play prominent roles in response arsenate. The microarray experiment revealed induction chloroplast Cu/Zn...
Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify quality RNA, there have been no large-scale empirical evaluations major determinants success. We used combination existing newly developed methods isolate total RNA from 1115 samples 695 plant species 324 families, which represents >900 million years phylogenetic diversity green algae through flowering plants, including many plants...