A5007968942- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Molecular Biology Techniques and Applications
- Plant Disease Resistance and Genetics
- Environmental DNA in Biodiversity Studies
- Plant chemical constituents analysis
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Plant Pathogens and Fungal Diseases
- Cell Image Analysis Techniques
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Botany, Ecology, and Taxonomy Studies
- Plant Stress Responses and Tolerance
- Phytase and its Applications
- Entomopathogenic Microorganisms in Pest Control
- Lipid metabolism and biosynthesis
- Ecology and Conservation Studies
- Polyamine Metabolism and Applications
- Postharvest Quality and Shelf Life Management
- Biological Control of Invasive Species
University of Tennessee at Knoxville
2016-2025
Biogen (United States)
2023-2025
South Dakota State University
2013-2014
Knoxville College
2013
The phytohormone auxin regulates nearly all aspects of plant growth and development. Based on the current model in Arabidopsis thaliana, Auxin/indole-3-acetic acid (Aux/IAA) proteins repress auxin-inducible genes by inhibiting response transcription factors (ARFs). Experimental evidence suggests that heterodimerization between Aux/IAA ARF are related to their unique biological functions. objective this study was generate Aux/IAA-ARF protein-protein interaction map using full length sequences...
Abstract Plant-parasitic cyst nematodes synthesize and secrete effector proteins that are essential for parasitism. One such protein is the 10A07 from sugar beet nematode, Heterodera schachtii, which exclusively expressed in nematode dorsal gland cell during all parasitic stages. Overexpression of H. schachtii Arabidopsis thaliana produced a hypersusceptible phenotype response to infection along with developmental changes reminiscent auxin effects. The physically associates plant kinase...
Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance PI88788 breaking down due to narrow genetic background and population shift. requires mainly rhg1-b locus, while 'Peking' rhg1-a Rhg4 for resistance. In present study, whole genome re-sequencing of 106 lines was used define Rhg haplotypes investigate their responses HG-Types. The analysis showed a comprehensive profile SNPs...
A growing body of evidence indicates that epigenetic modifications can provide efficient, dynamic, and reversible cellular responses to a wide range environmental stimuli. However, the significance in plant-pathogen interactions remains largely unexplored. In this study, we comprehensive analysis epigenome changes during compatible interaction between beet cyst nematode Heterodera schachtii Arabidopsis (Arabidopsis thaliana). Whole-genome bisulfite sequencing was conducted assess dynamic...
Summary Micro RNA s (mi s) are a major class of small non‐coding with emerging functions in biotic and abiotic interactions. Here, we report on new functional role Arabidopsis miR827 its NITROGEN LIMITATION ADAPTATION ( NLA ) target gene mediating plant susceptibility to the beet cyst nematode Heterodera schachtii . Cyst nematodes sedentary endoparasites that induce formation multinucleated feeding structures termed syncytia roots host plants. Using promoter: GUS fusion assays established...
Ethylene is a gaseous plant hormone perceived by family of receptors in Arabidopsis (Arabidopsis thaliana) including ETHYLENE RESPONSE1 (ETR1) and ETR2. Previously we showed that etr1-6 loss-of-function plants germinate better etr2-3 worse than wild-type under NaCl stress response to abscisic acid (ABA). In this study, expanded these results showing ETR1 ETR2 have contrasting roles the control germination variety inhibitory conditions for seed such as treatment with KCl, CuSO4, ZnSO4,...
Mitogen-activated protein kinase (MAPK) genes in eukaryotes regulate various developmental and physiological processes including those associated with biotic abiotic stresses. Although MAPKs some plant species Arabidopsis have been identified, they are yet to be identified soybean. Major objectives of this study were identify GmMAPKs, assess their evolutionary relationships, analyze functional divergence. We a total 38 MAPKs, eleven MAPKKs, 150 MAPKKKs Within the GmMAPK family, we also new...
MicroRNAs (miRNAs) recently have been established as key regulators of transcriptome reprogramming that define cell function and identity. Nevertheless, the molecular functions greatest number miRNA genes remain to be determined. Here, we report cooperative regulatory miR858 its MYB83 transcription factor target gene in during Heterodera cyst nematode parasitism Arabidopsis (Arabidopsis thaliana). Gene expression analyses promoter-GUS fusion assays documented a role posttranscriptional...
Soybean nodulation is a highly controlled process that involves complex gene regulation at both transcriptional and post-transcriptional levels. In the present study, we profiled expression changes, alternative splicing events, DNA methylation patterns during nodule formation, development, senescence. The transcriptome data uncovered key transcription of development included 9669 core genes 7302 stage-specific genes. Alternative analysis total 2323 undergo events in least one developmental...
Growth-regulating factors (GRFs) belong to a small family of transcription that are highly conserved in plants. GRFs regulate many developmental processes and plant responses biotic abiotic stimuli. Despite the importance GRFs, detailed mechanistic understanding their regulatory functions is still lacking. In this study, we used ChIP sequencing (ChIP-seq) identify genome-wide binding sites Arabidopsis GRF1 GRF3, correspondingly direct downstream target genes. RNA-sequencing (RNA-seq)...
Summary Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both GmSHMT08 and GmSNAP18 in ‘Peking’‐type . Here, we describe molecular interaction between GmSNAP18, which potentiated by a pathogenesis‐related protein GmPR08‐Bet VI. Like , VI expression was induced response to its overexpression decreased cysts 65% infected transgenic roots. Overexpression of did not have an effect on when two...
DNA methylation is a widespread epigenetic mark that affects gene expression and transposon mobility during plant development stress responses. However, the role of in regulating microRNA (miRNA) genes remains largely unexplored. Here, we analyzed changes miRNA using pair soybean (Glycine max) near-isogenic lines (NILs) differing their response to cyst nematode (SCN; Heterodera glycines). Differences global levels over SCN infection were observed between isogenic lines. with significant...
Abstract Mitogen‐activated protein kinase (MPK) cascades play central signalling roles in plant immunity and stress response. The soybean orthologue of MPK kinase2 (GmMKK2) was recently identified as a potential node whose expression is upregulated the feeding site induced by cyst nematode (SCN, Heterodera glycines ). To investigate role GmMKK2 soybean–SCN interactions, we overexpressed catabolically inactive variant referred to kinase‐dead (KD‐GmMKK2) using transgenic hairy roots. KD‐GmMKK2...
Abstract In soybeans, eighteen members constitute the serine hydroxymethyltransferase ( GmSHMT ) gene family, of which cytosolic-targeted GmSHMT08 c member has been reported to mediate resistance soybean cyst nematode (SCN). This work presents a comprehensive study SHMT family members, including synteny, phylogeny, subcellular localizations, haplotypes, protein homology modeling, mutational, and expression analyses. Phylogenetic analysis showed that genes are divided into four classes...
Summary Plant‐parasitic cyst nematodes successfully exploit various phytohormone signaling pathways to establish a new hormonal equilibrium that facilitates nematode parasitism. Although it is largely accepted ethylene regulates plant responses infection, mechanistic understanding of how shapes plant–nematode interactions remains unknown. In this study, we examined the involvement components regulating perception and in establishing Arabidopsis susceptibility Heterodera schachtii using large...
The cuprizone (CPZ) model is widely used for modeling demyelination in multiple sclerosis (MS) and testing potential remyelination therapies. We integrated single-cell spatial transcriptomics (ST) to fine map the cellular molecular responses during de remyelination. ST revealed global neuroinflammation brain beyond corpus callosum, with region-specific differences. identified oligodendroglia microglia as two major cell types significant transcriptomic changes model. Ligand‒receptor pairing...
Recent advances in spatial transcriptomics (ST) technologies have transformed our ability to profile gene expression while retaining the crucial context within tissues. However, existing ST platforms suffer from high costs, long turnaround times, low resolution, limited coverage, and small tissue capture areas, which hinder their broad applications. Here we present iSCALE, a method that predicts super-resolution automatically annotates cellular-level architecture for large-sized tissues...
Summary Plasmodesmata (PD) allow direct communication across the cellulosic plant cell wall, facilitating intercellular movement of metabolites and signaling molecules within symplast. In Arabidopsis thaliana embryos with reduced levels chloroplast RNA helicase ISE2, trafficking number branched PD were increased. We therefore investigated relationship between altered ISE2 expression trafficking. Gene analyses in tissues where was increased or decreased identified genes associated metabolism...
Summary Plant‐parasitic cyst nematodes induce the formation of a multinucleated feeding site in infected root, termed syncytium. Recent studies point to key roles phytohormone auxin regulation gene expression and establishment Nevertheless, information about spatiotemporal patterns transcription factors that mediate transcriptional responses during syncytium is limited. Here, we provide map 22 response ( ARFs ) initiation, maintenance stages induced by nematode H eterodera schachtii...
Developing soybean lines with high levels of stearic acid is a primary goal the industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to dual role in seeds and nodule development, all derived deleterious mutant alleles are extremely poor agronomic value because defective nodulation. The stearoyl-acyl carrier protein desaturase (GmSACPD) gene family composed five members. Comparative genomics analysis indicated that SACPD genes were duplicated from...
Resistance to soybean cyst nematodes (SCN) in “Peking-type” resistance is bigenic, requiring Rhg4-a and rhg1-a. encodes a serine hydroxymethyltransferase (GmSHMT08) rhg1-a soluble NSF attachment protein (GmSNAP18). Recently, it has been shown that pathogenesis-related protein, GmPR08-Bet VI, potentiates the interaction between GmSHMT08 GmSNAP18. Mutational analysis using spontaneously occurring ethyl methanesulfonate (EMS)-induced mutations was carried out increase our knowledge of...
BAK1-INTERACTING RECEPTOR LIKE KINASE1 (BIR1) is a negative regulator of various aspects disease resistance and immune responses. Here, we investigated the functional role soybean (Glycine max) BIR1 (GmBIR1) during interaction with cyst nematode (SCN, Heterodera glycines) molecular mechanism through which GmBIR1 regulates plant immunity. Overexpression wild-type variant (WT-GmBIR1) using transgenic hairy roots significantly increased susceptibility to SCN, whereas overexpression kinase-dead...
Protein kinases phosphorylate proteins for functional changes and are involved in nearly all cellular processes, thereby regulating almost aspects of plant growth development, responses to biotic abiotic stresses. We generated two independent co-expression networks soybean genes using control stress response gene expression data identified 392 differentially highly interconnected kinase hub among the networks. Of these kinases, 90 were as "syncytium connected hubs", potentially essential...
Abstract Gene co‐expression network analysis is an efficient systems biology approach for the discovery of novel gene functions and trait‐associated modules. To identify clusters functionally related genes involved in soybean nodule formation development, we performed a weighted analysis. Two nodule‐specific modules (NSM‐1 NSM‐2, containing 304 203 genes, respectively) were identified. The NSM‐1 promoters significantly enriched cis‐ binding elements ERF, MYB, C2H2‐type zinc transcription...