A5101795866- Cancer-related molecular mechanisms research
- Environmental DNA in Biodiversity Studies
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Schizophrenia research and treatment
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Cannabis and Cannabinoid Research
- CAR-T cell therapy research
- Diet and metabolism studies
- Stress Responses and Cortisol
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Melanoma and MAPK Pathways
Pfizer (United States)
2017-2025
Biogen (United States)
2019-2023
Abstract Psychiatric disorders such as schizophrenia (SCZ), bipolar disorder (BD), and major depressive (MDD) arise from complex interactions between genetic environmental factors. Common variants associated with multiple psychiatric suggest that shared architecture could contribute to divergent clinical syndromes. To evaluate transcriptional alterations across connected brain regions, Affymetrix microarrays were used profile postmortem dorsolateral prefrontal cortex (DLPFC), hippocampus,...
Many current gene therapy targets use recombinant adeno-associated virus (AAV). The majority of delivered AAV therapeutics persist as episomes, separate from host DNA, yet some viral DNA can integrate into in different proportions and at genomic locations. potential for integration leading to oncogenic transformation has led regulatory agencies require investigation events following preclinical species. In the present study, tissues were collected cynomolgus monkeys mice 6 8 weeks,...
Status epilepticus (SE) is a life-threatening and commonly drug-refractory condition. Novel therapies are needed to rapidly terminate seizures prevent mortality morbidity. Monoacylglycerol lipase (MAGL) the key enzyme responsible for hydrolysis of endocannabinoid 2-arachidonoylglycerol (2-AG) major contributor brain pool arachidonic acid (AA). Inhibiting monoacylglycerol modulates synaptic activity neuroinflammation, 2 mediators excessive neuronal activation underlying seizures. We studied...
The cuprizone (CPZ) model is widely used for modeling demyelination in multiple sclerosis (MS) and testing potential remyelination therapies. We integrated single-cell spatial transcriptomics (ST) to fine map the cellular molecular responses during de remyelination. ST revealed global neuroinflammation brain beyond corpus callosum, with region-specific differences. identified oligodendroglia microglia as two major cell types significant transcriptomic changes model. Ligand‒receptor pairing...
Immune checkpoint inhibitors (ICI) targeting CTLA-4 and PD-1 have shown remarkable antitumor efficacy, but can also cause immune-related adverse events, including inhibitor-induced liver injury (ChILI). This multi-omic study aimed to investigate changes in blood samples from treated cancer patients who developed ChILI. PBMCs were sequenced for by transcriptomic T cell receptor repertoire (bulk single-cell immune profiling), extracellular vesicle (EV) enrichment plasma was analyzed mass...
Abstract Background Single-cell RNA sequencing is a state-of-the-art technology to understand gene expression in complex tissues. With the growing amount of data being generated, standardization and automation analysis are critical generating hypotheses discovering biological insights. Results Here, we present scRNASequest, semi-automated single-cell RNA-seq (scRNA-seq) workflow which allows (1) preprocessing from raw UMI count data, (2) harmonization by one or multiple methods, (3)...
RNA medicines have become a promising platform for therapeutic use in recent years. Understanding the immunomodulatory effects of novel mRNA-LNPs is crucial future development. An vitro whole blood assay was developed to assess impact on immune cell function, cytokine release, and complement activation. significantly increased CD69 expression T cells natural killer (NK) cells, CD80/CD86 myeloid subsets, dose-dependent fashion. Furthermore, elicited robust release pro-inflammatory cytokines,...