A5058199613- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
University of Tennessee at Knoxville
2021-2024
Abstract Mitogen‐activated protein kinase (MPK) cascades play central signalling roles in plant immunity and stress response. The soybean orthologue of MPK kinase2 (GmMKK2) was recently identified as a potential node whose expression is upregulated the feeding site induced by cyst nematode (SCN, Heterodera glycines ). To investigate role GmMKK2 soybean–SCN interactions, we overexpressed catabolically inactive variant referred to kinase‐dead (KD‐GmMKK2) using transgenic hairy roots. KD‐GmMKK2...
BAK1-INTERACTING RECEPTOR LIKE KINASE1 (BIR1) is a negative regulator of various aspects disease resistance and immune responses. Here, we investigated the functional role soybean (Glycine max) BIR1 (GmBIR1) during interaction with cyst nematode (SCN, Heterodera glycines) molecular mechanism through which GmBIR1 regulates plant immunity. Overexpression wild-type variant (WT-GmBIR1) using transgenic hairy roots significantly increased susceptibility to SCN, whereas overexpression kinase-dead...
Protein kinases phosphorylate proteins for functional changes and are involved in nearly all cellular processes, thereby regulating almost aspects of plant growth development, responses to biotic abiotic stresses. We generated two independent co-expression networks soybean genes using control stress response gene expression data identified 392 differentially highly interconnected kinase hub among the networks. Of these kinases, 90 were as "syncytium connected hubs", potentially essential...
A growing body of evidence indicates that epigenetic mechanisms, particularly DNA methylation, play key regulatory roles in plant-nematode interactions. Nevertheless, the transcriptional activity genes mediating methylation and active demethylation nematode feeding sites remains largely unknown. Here, we profiled promoter 12 involved maintenance de novo establishment syncytia galls induced respectively by cyst Heterodera schachtii root-knot Meloidogyne incognita Arabidopsis roots. The assays...
Abstract Fluopyram is being used to manage plant-parasitic nematodes in cotton ( Gossypium hirsutum ) and soybean Glycine max ), but the duration depth of root protection from Meloidogyne incognita by seed-applied fluopyram unknown. Both M. susceptible cotton, Stoneville ‘ST 4848 GLT’, soybean, Delta Grow ‘DG 4880 GLY’, cultivars were treated with or abamectin inoculated second-stage juveniles two greenhouse studies. Root penetration was suppressed 7 21 d after planting while a similar trend...
Soybean cyst nematode (SCN, Heterodera glycines) is a serious root parasite of soybean (Glycine max) that induces extensive gene expression changes associated with pleiotropic biological activities in infected cells. However, the impacts various SCN Hg Types on host transcriptome reprogramming remain largely unknown. Here, we developed and used two recombinant inbred lines (RIL-72 RIL-137) to profile infection sites during resistant susceptible interactions Type 1.2.5.7 0. bioassays...