A5045979148- Renal cell carcinoma treatment
- Ferroptosis and cancer prognosis
- Neurobiology and Insect Physiology Research
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Invertebrate Immune Response Mechanisms
- Cancer Genomics and Diagnostics
- Insect and Arachnid Ecology and Behavior
- Renal and related cancers
- Glioma Diagnosis and Treatment
- Renal and Vascular Pathologies
- Genetic Neurodegenerative Diseases
- Cancer, Hypoxia, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
Washington University in St. Louis
2022-2025
James S. McDonnell Foundation
2023-2024
Duke University
2018
Abstract Identifying tumor-cell-specific markers and elucidating their epigenetic regulation spatial heterogeneity provides mechanistic insights into cancer etiology. Here, we perform snRNA-seq snATAC-seq in 34 28 human clear cell renal carcinoma (ccRCC) specimens, respectively, with matched bulk proteogenomics data. By identifying 20 tumor-specific through a multi-omics tiered approach, reveal an association between higher ceruloplasmin ( CP ) expression reduced survival. knockdown,...
Although genomic anomalies in glioblastoma (GBM) have been well studied for over a decade, its 5-year survival rate remains lower than 5%. We seek to expand the molecular landscape of high-grade glioma, composed IDH-wildtype GBM and IDH-mutant grade 4 astrocytoma, by integrating proteomic, metabolomic, lipidomic, post-translational modifications (PTMs) with transcriptomic measurements uncover multi-scale regulatory interactions governing tumor development evolution. Applying 14 proteogenomic...
In Drosophila, 50 classes of olfactory receptor neurons (ORNs) connect to class-specific and uniquely positioned glomeruli in the antennal lobe. Despite identification cell surface receptors regulating axon guidance, how ORN axons sort form stereotypical remains unclear. Here we show that heterophilic adhesion proteins, DIPs Dprs, are expressed ORNs during glomerular formation. Many express a unique combination DIPs/dprs, with same class expressing interacting partners, suggesting role...
Abstract Clear cell renal carcinoma (ccRCC) is the most prevalent subtype of carcinoma, accounting for approximately 70% cases. Strikingly, ccRCC exhibits a marked sex disparity in prevalence, being significantly more common men than women. This underscores importance systematically investigating differences to elucidate their implications diagnosis, prognosis, and therapeutic strategies ccRCC. To address this, we generated comprehensive single-nucleus sequencing atlas (snRNA-seq snATAC-seq)...
Abstract Renal cell carcinomas (RCC) are highly heterogeneous and aggressive cancers with diverse histological subtypes, molecular profiles, tumor microenvironments (TMEs), clinical behaviors. Intratumoral heterogeneity (ITH), a hallmark of RCC, arises from distinct subclones within the same that vary histopathologically molecularly. To elucidate biological mechanisms underlying RCC tumorigenesis at single-cell resolution, we examined 155 specimens 78 cases, encompassing clear (ccRCC),...
Abstract BAP1 mutations are associated with increased T cell and tumor-associated macrophage infiltration in clear renal carcinoma (ccRCC) predictive of poor prognosis. have also been found to be T-cell peritoneal mesothelioma uveal melanoma. Because known aggressive disease survival, we hypothesize that these lead a more immunosuppressive microenvironment. Using single nucleus RNA sequencing high-resolution spatial transcriptomics, confirmed mutant tumors immune hot high fraction Treg,...
Summary In Drosophila , 50 classes of olfactory receptor neurons (ORNs) connect to class-specific and uniquely positioned glomeruli in the antennal lobe. Despite identification cell surface receptors regulating axon guidance, how ORN axons sort form stereotypical remains unclear. Here we show that heterophilic adhesion proteins, DIPs Dprs, are expressed ORNs during glomerular formation. Each class expresses a unique combination / dprs with same expressing interacting partners, suggesting...
Abstract Aging is a common risk factor in neurodegenerative disorders and the ability to investigate aging of neurons an isogenic background would facilitate discovering interplay between neuronal onset neurodegeneration. Here, we perform direct reprogramming longitudinally collected human fibroblasts reveal genetic pathways altered at different ages. Comparative transcriptome analysis aged striatal medium spiny (MSNs), primary subtype affected Huntington’s disease (HD), identified...
Abstract The National Cancer Institute Clinical Proteomic Atlas Consortium (CPTAC) herein reports our deep characterization of 228 grade IV IDH1 WT and mutant astrocytomas (including 28 matched primary recurrent GBMs) using 15 proteogenomic metabolomic platforms. Major advances over first CPTAC GBM report (Wang et al., 2021, Cell), are the inclusion many more samples including paired tumors, application new platforms, glycoproteomics targeted mass spectrometry methods, development...
Abstract Clear cell renal carcinoma (ccRCC) is the most common type of kidney cancer, with 5-year mortality 25%. The molecular landscape ccRCC has been elucidated by a number next generation sequencing studies and complemented recent global proteome phosphoproteome results. However, limitation such mixture signals from distinct populations, presenting challenges to subsequent interpretation. As remedy, single nuclei RNA-seq (snRNA-seq) able dissect populations map their differential...
Abstract Clear cell renal carcinoma (ccRCC) appears as one of the most immune infiltrated tumors in pan-cancer comparisons. A systematic characterization microenvironment ccRCC is important for understanding disease progression and would be beneficial to predicting outcomes. To better understand cellular components within tumor (TME), interactions among different types, contribution outcomes, we collected 34 samples single-nuclei RNA-Seq (snRNASeq) profiling. We obtained 161312 cells total,...