A5045104429- Renal cell carcinoma treatment
- Advanced Chemical Sensor Technologies
- Olfactory and Sensory Function Studies
- Ferroptosis and cancer prognosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Phagocytosis and Immune Regulation
- Cancer therapeutics and mechanisms
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Renal and related cancers
- Biochemical Analysis and Sensing Techniques
- Chronic Obstructive Pulmonary Disease (COPD) Research
- CAR-T cell therapy research
- Diverse Scientific Research Studies
- Single-cell and spatial transcriptomics
- Synthesis and biological activity
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- Quinazolinone synthesis and applications
- Eosinophilic Disorders and Syndromes
- Chronic Myeloid Leukemia Treatments
- Retinoids in leukemia and cellular processes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Biosensing Techniques and Applications
Stanford University
2023-2025
Palo Alto University
2023
Washington University in St. Louis
2021-2023
James S. McDonnell Foundation
2021-2022
Technion – Israel Institute of Technology
2016-2019
Sri Venkateswara University
2014-2015
Sugen Life Sciences (India)
2014-2015
Nuclear factor kappa B (NFkB) is a transcription that resides in the cytoplasm of every cell and translocates to nucleus when activated. Its activation induced by wide variety agents including stress, cigarette smoke, viruses, bacteria, inflammatory stimuli, cytokines, free radicals, carcinogens, tumor promoters endotoxins. On activation, NFkB regulates expression almost 400 different genes, which include enzymes (COX-2 iNOS) cytokines (TNF, IL-1, IL-6,IL-8 chemokines),adhesion molecules,...
Pancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, transcriptomics cellular imaging. Subpopulations of tumor cells exhibited signatures proliferation, KRAS signaling, cell stress epithelial-to-mesenchymal transition. Mapping mutations copy number events distinguished populations normal transitional...
Abstract Identifying tumor-cell-specific markers and elucidating their epigenetic regulation spatial heterogeneity provides mechanistic insights into cancer etiology. Here, we perform snRNA-seq snATAC-seq in 34 28 human clear cell renal carcinoma (ccRCC) specimens, respectively, with matched bulk proteogenomics data. By identifying 20 tumor-specific through a multi-omics tiered approach, reveal an association between higher ceruloplasmin ( CP ) expression reduced survival. knockdown,...
PURPOSE Despite the effectiveness of immunotherapy in advanced melanoma, lack clinically reliable biomarkers hinders precision medicine approaches. This study investigates potential immune time-resolved Förster resonance energy transfer (iFRET) as a novel tool for understanding response melanoma. METHODS Using tissue from pilot phase II neoadjuvant talimogene laherparepvec (TVEC) before standard surgery, we explore iFRET's ability to assess PD-L1:PD-1 interactions tumor microenvironment...
Abstract Immunotherapy and targeted therapy have altered the standard of care for cancer patients, improving survival rates control, even in intransigent metastasis. Yet, only 20-40% patients respond to immunotherapy, up 50% experience severe adverse events, limiting application. Notably, cellular heterogeneity diversity makeup tumor immune microenvironment (TiME) present extraordinary challenges finding predictive biomarkers correlating therapeutic response. Existing profiling methods, with...
This study was designed to investigate the combination effects of brucine and gemcitabine, each with anticancer properties, in MCF-7 human breast cancer cells culture. With regard cell viability, both drugs their combinations were inversely proportional dose time. For various drug studied, analysed using CompuSyn software. The analyses revealed synergistic and/or additive regarding anchorage-independent growth migration. Combination exhibited diversified impacts type treatment, namely...
Idiopathic pulmonary fibrosis is a progressive fatal lung disease characterized by excessive collagen deposition, with no effective treatments. We investigated the efficacy of natural products high anti-inflammatory activity, such as passion fruit peel extract (PFPE), in mouse model bleomycin-induced (PF). C57BL/6J mice were subjected to single intratracheal instillation bleomycin induce PF. Daily PFPE treatment significantly reduced loss body mass and mortality rate compared those treated...
Introduction: Idiopathic pulmonary fibrosis (IPF) is characterized by alveolitis, progressing into fibrosis. Due to the involvement of both endothelin and platelet-derived growth factor signaling in IPF, combination effects a bosentan imatinib were studied mouse model bleomycin-induced Methods: Mice subjected bleomycin instillation (0.05 U) administered with either (100 mg/kg) and/or (50 mg/kg). Inflammatory cell count, total protein estimation bronchoalveolar lavage fluid, lung edema,...
Abstract Cell–cell cross talk is of great importance in cancer research due to its major role proliferation, differentiation, migration, and influence on the apoptotic pathway. Different cell–cell communication mechanisms have come mainly from proteomic genomic approaches. In this paper, a new route reported for between cells that occurs, even when they are far away each other. Single‐cell culture analysis shows upregulation emits hundreds volatile organic compounds (VOCs) into their...
SUMMARY Pancreatic Ductal Adenocarcinoma (PDAC) is a lethal disease with limited treatment options and poor survival. We studied 73 samples from 21 patients (7 treatment-naïve 14 treated neoadjuvant regimens), analyzing distinct spatial units performing bulk proteogenomics, single cell sequencing, cellular imaging. Spatial drivers, including mutant KRAS , SMAD4 GNAQ, were associated differential phosphosignaling metabolic responses compared to wild type. Single subtyping discovered 12 of...
<h3>Background</h3> Although survival remains significantly poorer for thick, ulcerated melanoma, application of immunotherapy is controversial in the absence nodal disease. Talimogene Laherparpvec (TVEC) only FDA approved intralesional therapy melanoma and has demonstrated overall response bystander effect (34% complete uninjected lesions) late stage melanoma. We hypothesize neoadjuvant TVEC II will treat local subclinical regional disease still allow standard care surgery. <h3>Methods</h3>...
<h3>Background</h3> Tumor-immune microenvironment (TiME) profiling methods, such as antibody-based labeling and RNA sequencing, have increased the complexity of analyses possible but are costly, time-intensive, do not faithfully capture structural or post-translational modifications that change functional behavior critical proteins. Here, we demonstrate rapid, single-cell differentiation state characterization in TiME using vibrational spectroscopy with metasurface optics (VISMO) machine...
In article number 1900131, Hossam Haick and co-workers report a new route for cross talk between cancer cells that occurs, even when they are far away from each other. The relies on volatile organic compounds (VOCs) generated by the cell exchanged with found in their vicinity or at long distances.
<h3>Background</h3> Anti-PD-1 refractory melanoma remains a devastating clinical challenge. We previously defined AXL tyrosine kinase as an emerging target of interest in tumor associated macrophage (TAM) behavior and immunotherapeutic resistance. Here, we aim to define the mechanisms by which mediates tumor-immune crosstalk capacity for inhibition address resistance checkpoint blockade. <h3>Methods</h3> Characterization mouse melanoma: YUMM1.7 (anti-PD-1 resistant), YUMMeR1.7, B16F10,...
Abstract Next-generation sequencing enabled molecular landscaping and the discovery of novel tumor markers in ccRCC. However, spatial relationships histological features microenvironment were lost during this process. Combining snRNA-seq, snATAC-seq, Spatial Transcriptomics (ST) technology, immunofluorescence labeling, we discovered ccRCC progression markers, uncovered their dependent expression pattern both human tumors patient-derived xenograft (PDX). Using snRNA-seq such as NDRG1, MGST1,...
Abstract The molecular landscape of clear cell renal carcinoma (ccRCC) has been annotated by a number large-scale sequencing studies, complemented recent proteomics efforts. However, these bulk analyses are limited the diverse populations that compose tumors. Here, we obtained single-nucleus RNA-seq (snRNA-seq) and ATAC-seq (snATAC-seq) data from 31 13 samples ccRCC patients, respectively, with matching proteogenomics collected through National Cancer Institute Clinical Proteomic Tumor...