A5100460981- Cancer Immunotherapy and Biomarkers
- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Immune cells in cancer
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Chromatin Remodeling and Cancer
- Cancer, Hypoxia, and Metabolism
Guilin Medical University
2022-2025
Tokushima University
2024-2025
Shenzhen Maternity and Child Healthcare Hospital
2022-2025
Southern Medical University
2017-2025
Guangdong Academy of Medical Sciences
2025
Guangdong Provincial People's Hospital
2025
Ministry of Agriculture and Rural Affairs
2023-2025
Fujian Agriculture and Forestry University
2021-2025
Hangzhou Hospital of Traditional Chinese Medicine
2025
Zhejiang Chinese Medical University
2020-2025
Pancreatic ductal adenocarcinoma is a lethal disease with limited treatment options and poor survival. We studied 83 spatial samples from 31 patients (11 treatment-naïve 20 treated) using single-cell/nucleus RNA sequencing, bulk-proteogenomics, transcriptomics cellular imaging. Subpopulations of tumor cells exhibited signatures proliferation, KRAS signaling, cell stress epithelial-to-mesenchymal transition. Mapping mutations copy number events distinguished populations normal transitional...
Clear cell renal carcinomas (ccRCCs) represent ∼75% of RCC cases and account for most RCC-associated deaths. Inter- intratumoral heterogeneity (ITH) results in varying prognosis treatment outcomes. To obtain the comprehensive profile ccRCC, we perform integrative histopathologic, proteogenomic, metabolomic analyses on 305 ccRCC tumor segments 166 paired adjacent normal tissues from 213 cases. Combining histologic molecular profiles reveals ITH 90% ccRCCs, with 50% demonstrating immune...
Abstract Oncolytic virotherapy holds promise for cancer treatment, but the factors determining its oncolytic activity remain unclear. Neutrophil extracellular traps (NETs) are associated with progression, yet their formation mechanism and role in elusive. In this study, we demonstrate that, glioma, upregulation of IGF2BP3 enhances expression E3 ubiquitin protein ligase MIB1, promoting FTO degradation via ubiquitin-proteasome pathway. This results increased m6A-mediated CSF3 release NET...
To study the spatial interactions among cancer and non-cancer cells
Lactate derived from aerobic glycolysis is crucial for DNA damage repair and chemoresistance. Nevertheless, it frequently noted that cancer cells depend on glutaminolysis to replenish essential metabolites. Whether how might enhance lactate production facilitate in remains unknown. Here, shown malate enzyme 2 (ME2), which metabolizes glutamine-derived pyruvate, contributes chemotherapy resistance ovarian cancer. Mechanistically, reduces the expression of glucose transporters impairs uptake...
Activated T lymphocytes are present in human atherosclerotic lesions and autoantibodies to antigens within have been detected serum, but the roles of cellular humoral immune systems atherogenesis not determined. The effect total lymphocyte deficiency on was investigated by crossing apo E-deficient mice (which develop atherosclerosis resembling disease) with deficient RAG2 is required for normal B development). Mice were placed a fat- cholesterol-enriched diet 12 wk. RAG2-deficient had no...
CD73 is a novel immune checkpoint associated with adenosine metabolism that promotes tumor progression by suppressing antitumor response and promoting angiogenesis. The inhibition of CD73, in combination blockade, targeted therapy or conventional therapy, improves effects numerous preclinical mouse models cancer. Emerging evidence suggests the anti-CD73 blockade has promising clinical activity patients advanced solid tumors. In this review, we will discuss specific role on both cells...
Abstract The role of IL-33, particularly in tumor growth and immunity, remains ill-defined. We show that exogenous IL-33 can induce robust antitumor effect through a CD8+ T cell–dependent mechanism. Systemic administration rIL-33 alone was sufficient to inhibit established tumors transplant de novo melanoma tumorigenesis models. Notably, addition direct action on cell expansion IFN-γ production, therapy activated myeloid dendritic cells (mDCs) tumor-bearing mice, restored activity, increased...
Abstract Agonist antibodies (Ab) directed against costimulatory molecules on the surface of antigen-primed T cells are in various stages pre-clinical and clinical trials, albeit with limited therapeutic benefit as single agents. The underlying mechanisms action remain incompletely understood. Here, we demonstrate an inhibitory role ecto-enzyme CD73 for agonistic anti-4-1BB/CD137 Ab therapy. In particular, anti-4-1BB treatment preferentially drives − effector cell response tumor inhibition....
Background Limited therapeutic options are available for triple-negative breast cancer (TNBC), emphasizing an urgent need more effective treatment approaches. The development of strategies by targeting tumor-associated macrophages (TAMs) to stimulate their ability Programmed Cell Removal (PrCR) provides a promising new immunotherapy TNBC treatment. Methods CD47 is critical self-protective “don’t eat me” signal on multiple human cancers against macrophage immunosurveillance. Using and mouse...
Abstract Background Transfer RNA-derived fragments (tRFs) are a new class of small non-coding RNAs. Recent studies suggest that tRFs participate in some pathological processes. However, the biological functions and mechanisms non-small cell lung cancer (NSCLC) largely unknown. Methods Differentially expressed were identified by tRF tiRNA sequencing using 9 pairs pre- post-operation plasma from patients with NSCLC. Quantitative real-time PCR (qRT-PCR) fluorescence situ hybridization (FISH)...
BackgroundImmune checkpoint inhibitors (ICIs) have modest activity in ovarian cancer (OC). To augment their activity, we used priming with the hypomethylating agent guadecitabine a phase II study.MethodsEligible patients had platinum-resistant OC, normal organ function, measurable disease, and received up to 5 prior regimens. The treatment included (30 mg/m2) on days 1-4, pembrolizumab (200 mg i.v.) day 5, every 21 days. primary endpoint was response rate. Tumor biopsies, plasma, PBMCs were...
Abstract Antibody-drug conjugates (ADCs) are a class of innovative biopharmaceutical drugs, which, via their antibody (mAb) component, deliver and release potent warhead (a.k.a. payload) at the disease site, thereby simultaneously improving efficacy delivered therapy reducing its off-target toxicity. To design ADCs promising efficacy, it is crucial to have critical data pharma-information biological activities for each ADC. However, no such database has been constructed yet. In this study,...
Abstract Identifying tumor-cell-specific markers and elucidating their epigenetic regulation spatial heterogeneity provides mechanistic insights into cancer etiology. Here, we perform snRNA-seq snATAC-seq in 34 28 human clear cell renal carcinoma (ccRCC) specimens, respectively, with matched bulk proteogenomics data. By identifying 20 tumor-specific through a multi-omics tiered approach, reveal an association between higher ceruloplasmin ( CP ) expression reduced survival. knockdown,...
Non-clear cell renal carcinomas (non-ccRCCs) encompass diverse malignant and benign tumors. Refinement of differential diagnosis biomarkers, markers for early prognosis aggressive disease, therapeutic targets to complement immunotherapy are current clinical needs. Multi-omics analyses 48 non-ccRCCs compared with 103 ccRCCs reveal proteogenomic, phosphorylation, glycosylation, metabolic aberrations in RCC subtypes. RCCs high genome instability display overexpression IGF2BP3 PYCR1. Integration...
Abstract miR155 is a regulator of immune cell development and function that generally thought to be immunostimulatory. However, we report here genetic ablation renders mice resistant chemical carcinogenesis the growth several transplanted tumors, suggesting functions in immunosuppression tumor promotion. Host deficiency promoted overall antitumor immunity despite finding defective responses miR155-deficient dendritic cells T cells. Further analysis compartments revealed regulated...
Summary Platelet counts ( PC ) estimate bleeding risk in Immune Thrombocytopenia ITP ). We investigated whether measures of thromboelastometry and absolute immature platelet fraction (A‐ IPF would correlate better with acute score ABS than or mean volume MPV Simultaneous determination , complete blood count was performed 141 patients; 112 underwent A‐ testing. Subgroup analyses were for paediatric subjects, <60 × 10 9 /l <30 /l. significantly inversely correlated all total cohort. did...
Abstract Although a number of studies have recently explored the contribution adaptive immunity in IL-33–mediated antitumor effects, innate immune involvement has been poorly characterized. Utilizing Rag1−/− mice (lacking T and B lymphocytes), we show this study that either systemic administration recombinant IL-33 or ectopic expression melanoma cells is sufficient to inhibit tumor growth independent immunity. We demonstrated effects depend on expansion activation NK cells. Interestingly,...
Abstract Accumulating evidence suggests that inhibiting adenosine-generating ecto-enzymes (CD39 and CD73) and/or adenosine A2A or A2B receptors (R) stimulates antitumor immunity limits tumor progression. Although activating A2ARs A2BRs causes similar immunosuppressive protumoral functions, few studies have investigated the distinct role of A2BR in cancer. Here, we showed expression by hematopoietic cells was primarily responsible for promoting growth. Deletion profoundly enhanced anticancer...
Radiation-induced brain injury (RIBI) is the most serious complication of radiotherapy in patients with head and neck tumors, which seriously affects quality life. Currently, there no effective treatment for RIBI, identifying new that targets pathological mechanisms RIBI urgently needed.Immunofluorescence staining, western blotting, quantitative real-time polymerase chain reaction (Q-PCR), co-culture primary neurons microglia, terminal deoxynucleotidyl transferase dUTP nick-end labeling...
Despite the rapid utilization of immunotherapy, emerging challenges to current immune checkpoint blockade need be resolved. Here, we report that elevation CD73 levels due its aberrant turnover is correlated with poor prognosis in immune-cold triple-negative breast cancers (TNBCs). We have identified TRIM21 as an E3 ligase governs destruction. Disruption stabilizes turn enhances CD73-catalyzed production adenosine, resulting suppression CD8 + T cell function. Replacement lysine 133, 208, 262,...