A5037253743- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Seismic Waves and Analysis
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Seismology and Earthquake Studies
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Fungal Infections and Studies
- Advanced Proteomics Techniques and Applications
- Trypanosoma species research and implications
- Solar and Space Plasma Dynamics
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Antifungal resistance and susceptibility
- Diabetes Management and Research
- Legionella and Acanthamoeba research
- Mass Spectrometry Techniques and Applications
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- Research on Leishmaniasis Studies
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Genomics and Phylogenetic Studies
- Diet, Metabolism, and Disease
Pacific Northwest National Laboratory
2016-2025
Richland College
2017-2024
Purdue University West Lafayette
2013-2023
Battelle
2011-2023
Government of the United States of America
2023
University of Chicago
2023
University of Colorado Anschutz Medical Campus
2023
Johns Hopkins University
2019
The University of Texas at El Paso
2006-2015
Universidade de São Paulo
2005-2012
ABSTRACT Cryptococcus neoformans produces vesicles containing its major virulence factor, the capsular polysaccharide glucuronoxylomannan (GXM). These cross cell wall to reach extracellular space, where is supposedly used for capsule growth or delivered into host tissues. In present study, we characterized vesicle morphology and protein composition by a combination of techniques including electron microscopy, proteomics, enzymatic activity, serological reactivity. Secretory in C. appear be...
Vesicular secretion of macromolecules has recently been described in the basidiomycete Cryptococcus neoformans, raising question as to whether ascomycetes similarly utilize vesicles for transport. In present study, we examine clinically important ascomycete Histoplasma capsulatum produce and utilized these structures secrete macromolecules. Transmission electron microscopy (TEM) shows transcellular by yeast cells. Proteomic lipidomic analyses isolated from culture supernatants reveal a rich...
The release of extracellular vesicles (EV) by fungal organisms is considered an alternative transport mechanism to trans-cell wall passage macromolecules. Previous studies have revealed the presence EV in culture supernatants from pathogens, such as Cryptococcus neoformans, Histoplasma capsulatum, Paracoccidioides brasiliensis, Sporothrix schenckii, Malassezia sympodialis and Candida albicans. Here we investigated size, composition, kinetics internalization bone marrow-derived murine...
Background Extracellular vesicles in yeast cells are involved the molecular traffic across cell wall. In pathogens, these have been implicated transport of proteins, lipids, polysaccharide and pigments to extracellular space. Cellular pathways required for biogenesis largely unknown. Methodology/Principal Findings We characterized vesicle production wild type (WT) mutant strains model Saccharomyces cerevisiae using transmission electron microscopy combination with light scattering analysis,...
Integrative multi-omics analyses can empower more effective investigation and complete understanding of complex biological systems. Despite recent advances in a range omics analyses, multi-omic measurements the same sample are still challenging current methods have not been well evaluated terms reproducibility broad applicability. Here we adapted solvent-based method, widely applied for extracting lipids metabolites, to add proteomics mass spectrometry-based measurements. The metabolite,...
Exosomes are 30–100-nm membrane vesicles of endocytic origin that released after the fusion multivesicular bodies (MVBs) with plasma membrane. While initial studies suggested role exosomes was limited to removal proteins during maturation reticulocytes erythrocytes, recent indicate they produced by different types cells and involved in promoting inter-cellular communication antigen presentation. Here, we describe isolation characterization from peripheral blood BALB/c mice infected...
Microorganisms release effector molecules that modulate the host machinery enabling survival, replication, and dissemination of a pathogen. Here we characterized extracellular proteome Paracoccidioides brasiliensis at its pathogenic yeast phase. Cell-free culture supernatants from Pb18 isolate, cultivated in defined medium, were separated into vesicle vesicle-free fractions, digested with trypsin, analyzed by liquid chromatography-tandem mass spectrometry. In preparations identified,...
Article26 June 2012Open Access Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation Aarash Bordbar Department Bioengineering, University California San Diego, La Jolla, CA, USA Search for more papers by this author Monica L Mo Ernesto S Nakayasu Pacific Northwest National Laboratory, Richland, WA, Alexandra C Schrimpe-Rutledge Young-Mo Kim Thomas O Metz Marcus B Jones J. Craig Venter Institute, Rockville, MD, Bryan Frank Richard D Smith Scott N...
Characterization of the mature protein complement in cells is crucial for a better understanding cellular processes on systems-wide scale. Toward this end, we used single-dimension ultra–high-pressure liquid chromatography mass spectrometry to investigate comprehensive “intact” proteome Gram-negative bacterial pathogen Salmonella Typhimurium. Top-down proteomics analysis revealed 563 unique proteins including 1,665 proteoforms generated by posttranslational modifications (PTMs), representing...
Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive secrete extracellular (EVs) was not pursued until recently due to assumption thick peptidoglycan cell wall would prevent their release environment. However, following discovery in fungi, which also walls, EVs now described variety of bacteria. purified from are implicated virulence, toxin release, and transference host cells, eliciting immune responses,...
Background Fungal extracellular vesicles are able to cross the cell wall and transport molecules that help in nutrient acquisition, defense, modulation of host defense machinery. Methodology/Principal Findings Here we present a detailed lipidomic analysis released by Paracoccidioides brasiliensis at yeast pathogenic phase. We compared data two representative isolates, Pb3 Pb18, which have distinct virulence profiles phylogenetic background. Vesicle lipids were fractionated into different...
ABSTRACT Lysine acetylation is a common protein post-translational modification in bacteria and eukaryotes. Unlike phosphorylation, whose functional role signaling has been established, it unclear what regulatory mechanism plays whether conserved across evolution. By performing proteomic analysis of 48 phylogenetically distant bacteria, we discovered sites on catalytically essential lysine residues that are invariant throughout removes the residue’s charge changes shape pocket required for...
We describe the first comprehensive analysis of midgut metabolome Aedes aegypti, primary mosquito vector for arboviruses such as dengue, Zika, chikungunya and yellow fever viruses. Transmission these viruses depends on their ability to infect, replicate disseminate from several tissues in vector. The metabolic environments within play crucial roles processes. Since are enveloped, viral replication, assembly release occur cellular membranes primed through manipulation host metabolism....
Abstract Interferon-α (IFNα), a type I interferon, is expressed in the islets of 1 diabetic individuals, and its expression signaling are regulated by T1D genetic risk variants viral infections associated with T1D. We presently characterize human beta cell responses to IFNα combining ATAC-seq, RNA-seq proteomics assays. The initial response characterized chromatin remodeling, followed changes transcriptional translational regulation. induces alternative splicing (AS) first exon usage,...
Histoplasma capsulatum produces extracellular vesicles containing virulence-associated molecules capable of modulating host machinery, benefiting the pathogen. Treatment H. cells with monoclonal antibodies (MAbs) can change outcome infection in mice. We evaluated sizes, enzymatic contents, and proteomic profiles released by fungal treated either protective MAb 6B7 (IgG1) or nonprotective 7B6 (IgG2b), both which bind heat shock protein 60 (Hsp60). Our results showed that treatment was...