A5057666213- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Biochemical and Structural Characterization
- Microbial Natural Products and Biosynthesis
- Vibrio bacteria research studies
- Inflammasome and immune disorders
- Orthopedic Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Dental materials and restorations
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Calcium signaling and nucleotide metabolism
- Escherichia coli research studies
- DNA and Nucleic Acid Chemistry
- Inhalation and Respiratory Drug Delivery
- Plant-Microbe Interactions and Immunity
University of North Carolina at Chapel Hill
2014-2025
Lung Institute
2017-2025
Northeastern University
2013-2019
Ollscoil na Gaillimhe – University of Galway
2014
University College Dublin
2010-2014
ABSTRACT Persisters are dormant variants that form a subpopulation of cells tolerant to antibiotics. largely responsible for the recalcitrance chronic infections therapy. In Escherichia coli , one widely accepted model persister formation holds stochastic accumulation ppGpp causes activation Lon protease degrades antitoxins; active toxins then inhibit translation, resulting in dormant, drug-tolerant persisters. We found various stresses induce toxin-antitoxin (TA) expression but induction...
Chronic coinfections of Staphylococcus aureus and Pseudomonas aeruginosa frequently fail to respond antibiotic treatment, leading significant patient morbidity mortality. Currently, the impact interspecies interaction on S. susceptibility remains poorly understood. In this study, we utilize a panel P. burn wound cystic fibrosis (CF) lung isolates demonstrate that alters bactericidal antibiotics in variable, strain-dependent manner further identify 3 independent interactions responsible for...
Persister cells are rare phenotypic variants that able to survive antibiotic treatment. Unlike resistant bacteria, which have specific mechanisms prevent antibiotics from binding their targets, persisters evade killing by entering a tolerant nongrowing state. Persisters been implicated in chronic infections multiple species, and growing evidence suggests persister responsible for many cases of treatment failure. New strategies aim kill more effectively, but the mechanism tolerance has...
Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Despite appropriate antibiotic treatment, relapse of GNB-BSI with the same strain is associated poor clinical outcomes high healthcare costs. The role persister cells, which sub-populations bacteria that survive for prolonged periods in presence bactericidal antibiotics, unclear. Using a cohort patients relapsed GNB-BSI, we aimed to determine how pathogen evolves within patient between initial...
ABSTRACT Teixobactin represents the first member of a newly discovered class antibiotics that act through inhibition cell wall synthesis. binds multiple bactoprenol-coupled precursors, inhibiting both peptidoglycan and teichoic acid Here, we show impressive bactericidal activity teixobactin is due to synergistic targets, resulting in damage, delocalization autolysins, subsequent lysis. We also find does not bind mature peptidoglycan, further increasing its at high densities against...
Bacterial invasion of synovial joints, as in infectious or septic arthritis, can be difficult to treat both veterinary and human clinical practice. Biofilms, the form free-floating clumps aggregates, are involved with pathogenesis arthritis periprosthetic joint infection (PJI). Infection a containing an orthopedic implant additionally complicate these infections due presence adherent biofilms. Because biofilm phenotypes, bacteria within infected joints show increased antimicrobial tolerance...
The icaADBC-encoded polysaccharide intercellular adhesin (PIA) and wall teichoic acids (WTA) are structural components of Staphylococcus epidermidis biofilms. Deletion tagO, which encodes the first enzymic step in WTA biosynthesis, had pleiotropic effects, including enhanced aggregation autolytic activity, impaired biofilm production. biofilm-negative phenotype tagO mutant, named TAGO1, was associated with increased cell surface hydrophobicity, lower rates primary attachment to polystyrene,...
ABSTRACT The polysaccharide intercellular adhesin or the cell wall-anchored accumulation-associated protein (Aap) mediates cellular accumulation during Staphylococcus epidermidis biofilm maturation. Mutation of sortase, which anchors up to 11 proteins (including Aap) wall, blocked development by cerebrospinal fluid isolate CSF41498. Aap was implicated in this phenotype when Western blots and two-dimensional (2D) electrophoresis revealed increased levels culture supernatants. Unexpectedly,...
Individuals with diabetes mellitus frequently develop severe skin and soft tissue infections (SSTIs) that are recalcitrant to antibiotic treatment. We examined how affects the emergence of resistance in a Staphylococcus aureus SSTI. determined S. evolves rapidly diabetic mice, while did not occur nondiabetic mice over course infection. Diabetes-associated immune cell dysfunction plays minor role resistance, hyperglycemia dominant facilitating expansion takeover resistant mutants infections....
Staphylococcus aureus is a leading human pathogen that frequently causes chronic and relapsing infections. Antibiotic-tolerant persister cells contribute to frequent antibiotic failure in patients. Macrophages represent an important niche during S. bacteremia, recent work has identified role for oxidative burst the formation of antibiotic-tolerant aureus. We find host-derived peroxynitrite, reaction product superoxide nitric oxide, main mediator tolerance macrophages. Using collection...
Antibiotic tolerance and antibiotic resistance are the two major obstacles to efficient reliable treatment of bacterial infections. Identifying adjuvants that sensitize resistant tolerant bacteria killing may lead development superior treatments with improved outcomes. Vancomycin, a lipid II inhibitor, is frontline for treating methicillin-resistant Staphylococcus aureus other Gram-positive However, vancomycin use has led increasing prevalence strains reduced susceptibility vancomycin. Here,...