A5046773391- Bacterial biofilms and quorum sensing
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Tracheal and airway disorders
- Bacterial Infections and Vaccines
- Solar and Space Plasma Dynamics
- Astrophysics and Cosmic Phenomena
- Geophysics and Gravity Measurements
- Pediatric health and respiratory diseases
- Gut microbiota and health
- Neonatal Respiratory Health Research
- Biochemical and Structural Characterization
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- Yersinia bacterium, plague, ectoparasites research
- COVID-19 Clinical Research Studies
- Pneumonia and Respiratory Infections
- Wound Healing and Treatments
- Genomics and Phylogenetic Studies
- Immune Response and Inflammation
University of North Carolina at Chapel Hill
2016-2025
Lung Institute
2017-2025
Children’s Minnesota - St. Paul Hospital
2023
Cincinnati Children's Hospital Medical Center
2023
National Institutes of Health
1998-2020
Center for Cancer Research
2020
National Cancer Institute
2020
University of California, Davis
2018
University of North Carolina Health Care
2009-2015
Bipar
2008
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute...
Pulmonary infection in cystic fibrosis (CF) is polymicrobial and it possible that anaerobic bacteria, not detected by routine aerobic culture methods, reside within infected airway mucus.To determine whether bacteria are present the sputum of patients with CF.Sputum samples were collected from clinically stable adults CF bronchoalveolar lavage fluid (BALF) children CF. Induced healthy volunteers who did have All processed using bacteriologic techniques quantified identified.Anaerobic species...
Summary The single polar flagellum of Pseudomonas aeruginosa is an important virulence and colonization factor this opportunistic pathogen. In study, the annotation genes belonging to fla regulon was updated their organization analysed in strains PAK PAO1, representative type‐a type‐b P. respectively. flagellar are clustered three non‐contiguous regions chromosome. A polymorphic locus flanked by flgJ fleQ Region I contains a glycosylation island PAK. expression ordered assembly complex...
Pseudomonas aeruginosa is a ubiquitous environmental bacterium capable of causing variety life-threatening human infections. The genetic basis for preferential infection certain immunocompromised patients or individuals with cystic fibrosis by P. not understood. To establish whether variation in the genomic repertoire strains can be associated particular type infection, we used whole-genome DNA microarray to determine genome content 18 isolated from most common infections and sources. A...
Cystic fibrosis (CF) is characterized by defective mucociliary clearance and chronic airway infection a complex microbiota. Infection, persistent inflammation periodic episodes of acute pulmonary exacerbation contribute to an irreversible decline in CF lung function. While the factors leading exacerbations are poorly understood, antibiotic treatment can temporarily resolve symptoms partially restore Previous studies indicated that may be associated with changes microbial densities...
Rationale: Characterization of bacterial populations in infectious respiratory diseases will provide improved understanding the relationship between lung microbiota, disease pathogenesis, and treatment outcomes.Objectives: To comprehensively define microbiota composition during stable exacerbation patients with bronchiectasis.Methods: Sputum was collected from when clinically before after completion antibiotic exacerbations. Bacterial abundance community were analyzed using anaerobic culture...
Biofilms are surface-attached multicellular communities. Using single-cell tracking microscopy, we showed that a pilY1 mutant of Pseudomonas aeruginosa is defective in early biofilm formation. We leveraged the observation PilY1 protein levels increase on surface to perform genetic screen identify mutants altered surface-grown expression this protein. Based our studies, found soon after initiating growth, cyclic AMP (cAMP) increase, dependent PilJ, chemoreceptor-like Pil-Chp complex, and type...
Although destructive airway disease is evident in young children with cystic fibrosis (CF), little known about the nature of early CF lung environment triggering disease. To elucidate pulmonary pathophysiology, we performed mucus, inflammation, metabolomic, and microbiome analyses on bronchoalveolar lavage fluid (BALF) from 46 preschool enrolled Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST CF) program 16 non-CF controls. Total mucins were elevated compared to...
It is unclear how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leads to the strong but ineffective inflammatory response that characterizes Coronavirus disease 2019 (COVID-19), with amplified immune activation in diverse cell types, including cells without angiotensin-converting enzyme receptors necessary for infection. Proteolytic degradation of SARS-CoV-2 virions a milestone host viral clearance, impact remnant peptide fragments from high loads not known. Here, we...
Pseudomonas aeruginosa (PA) is a ubiquitous, Gram-negative, bacteria that can attribute its survivability to numerous sensing and signaling pathways; conferring fitness due speed of response. Post-transcriptional regulation an energy efficient approach quickly shift gene expression in response the environment. The conserved post-transcriptional regulator RsmA involved regulating translation genes pathways contribute virulence, metabolism, antibiotic resistance. Prior high-throughput...
Neisseria gonorrhoeae , the Gram‐negative aetiological agent of gonorrhoea, is one many mucosal pathogens man that expresses competence for natural transformation. Expression this phenotype by gonococci appears to rely on expression type IV pili (Tfp), but mechanistic basis relationship remains unknown. During studies gonococcal pilus biogenesis, a homologue PilT family proteins, required Tfp‐dependent twitching motility in Pseudomonas aeruginosa and social gliding Myxococcus xanthus was...
Many respiratory pathogens, including Hemophilus influenzae, Streptococcus pneumoniae, and Pseudomonas aeruginosa, express neuraminidases that can cleave α2,3-linked sialic acids from glycoconjugates. As mucosal surfaces are heavily sialylated, have been thought to modify epithelial cells by exposing potential bacterial receptors. However, in contrast neuraminidase produced the influenza virus, a role for pathogenesis has not yet clearly established. We constructed mutant of P. aeruginosa...
<h3>Background</h3> Anaerobic bacteria are increasingly regarded as important in cystic fibrosis (CF) pulmonary infection. The aim of this study was to determine the effect antibiotic treatment on aerobic and anaerobic microbial community diversity abundance during exacerbations patients with CF. <h3>Methods</h3> Sputum collected at start completion when clinically stable. Bacteria were quantified identified following culture, composition also examined using culture-independent methods....
Chronic coinfections of Staphylococcus aureus and Pseudomonas aeruginosa frequently fail to respond antibiotic treatment, leading significant patient morbidity mortality. Currently, the impact interspecies interaction on S. susceptibility remains poorly understood. In this study, we utilize a panel P. burn wound cystic fibrosis (CF) lung isolates demonstrate that alters bactericidal antibiotics in variable, strain-dependent manner further identify 3 independent interactions responsible for...
Summary Multiple virulence systems in the opportunistic pathogen Pseudomonas aeruginosa are regulated by second messenger signalling molecule adenosine 3′, 5′‐cyclic monophosphate (cAMP). Production of cAMP putative adenylate cyclase enzyme CyaB represents a critical control point for gene regulation. To identify regulators CyaB, we screened transposon insertion library mutants with reduced intracellular cAMP. The majority insertions resulting mapped to Chp cluster encoding chemotaxis‐like...
The cystic fibrosis (CF) lung microbiome has been studied in children and adults; however, little is known about its relationship to early disease progression. To better understand the between respiratory disease, we characterized lower airways using bronchoalveolar lavage (BAL) samples obtained from clinically stable CF infants preschoolers who underwent bronchoscopy chest computed tomography (CT). Cross-sectional suggested a progression of with age, beginning relatively sterile infancy. By...
The e-liquids used in electronic cigarettes (E-cigs) consist of propylene glycol (PG), vegetable glycerin (VG), nicotine, and chemical additives for flavoring. There are currently over 7,700 e-liquid flavors available, while some have been tested toxicity the laboratory, most not. Here, we developed a 3-phase, 384-well, plate-based, high-throughput screening (HTS) assay to rapidly triage validate multiple e-liquids. Our data demonstrated that PG/VG vehicle adversely affected cell viability...
Anaerobic bacteria are present in large numbers the airways of people with cystic fibrosis (PWCF). In gut, anaerobes produce short-chain fatty acids (SCFAs) that modulate immune and inflammatory processes.To investigate capacity to contribute (CF) airway pathogenesis via SCFAs.Samples 109 PWCF were processed using anaerobic microbiological culture identified by 16S RNA sequencing. SCFA levels supernatants bronchoalveolar lavage (BAL) determined gas chromatography. The mRNA and/or protein...
Highlights•In silico predictions of miR-2392 as a miRNA involved with SARS-CoV-2•Overexpression produces similar biological response COVID-19 infection•miR-2392 is confirmed to circulate in serum and urine patients COVID-19•Development initiated potential antiviral therapeutic against COVID-19SummaryMicroRNAs (miRNAs) are small non-coding RNAs post-transcriptional gene regulation that have major impact on many diseases provide an exciting avenue toward therapeutics. From patient...