A5042997375- Sinusitis and nasal conditions
- Head and Neck Surgical Oncology
- Nasal Surgery and Airway Studies
- Cystic Fibrosis Research Advances
- Protein Kinase Regulation and GTPase Signaling
- Olfactory and Sensory Function Studies
- Allergic Rhinitis and Sensitization
- Tracheal and airway disorders
- Receptor Mechanisms and Signaling
- Asthma and respiratory diseases
- Advanced Chemical Sensor Technologies
- Meningioma and schwannoma management
- Oral and Maxillofacial Pathology
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Biochemical Analysis and Sensing Techniques
- Amoebic Infections and Treatments
- Inhalation and Respiratory Drug Delivery
- Diversity and Career in Medicine
- Cleft Lip and Palate Research
- SARS-CoV-2 and COVID-19 Research
- Pancreatitis Pathology and Treatment
- Pituitary Gland Disorders and Treatments
- Neonatal Respiratory Health Research
- Head and Neck Cancer Studies
University of North Carolina at Chapel Hill
2016-2025
Lung Institute
2019-2025
University of North Carolina at Charlotte
2024
Triological Society
2024
University of North Carolina Hospitals
2014-2022
Cystic Fibrosis Research Foundation
2022
Copiah-Lincoln Community College
2022
Memorial Hospital
2014-2021
University of North Carolina Health Care
2013-2020
Evidence Based Research (United States)
2013
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute...
Heterotrimeric G protein signaling is important for cell-proliferative and glucose-sensing signal transduction pathways in the model plant organism Arabidopsis thaliana . AtRGS1 a seven-transmembrane, RGS domain-containing that putative membrane receptor d -glucose. Here we show, by using FRET, -glucose alters interaction between AtGPA1 vivo unique heterotrimeric α subunit constitutively GTP-bound given its high spontaneous nucleotide exchange coupled with slow GTP hydrolysis. Analysis of...
Regulator of G protein signaling (RGS) proteins accelerate GTP hydrolysis by Galpha subunits and thus facilitate termination initiated protein-coupled receptors (GPCRs). RGS hold great promise as disease intervention points, given their signature role negative regulators GPCRs-receptors to which the largest fraction approved medications are currently directed. share a hallmark domain that interacts most avidly with when in its transition state for hydrolysis; binding stabilizing switch...
Abstract Topical intra-nasal sprays are amongst the most commonly prescribed therapeutic options for sinonasal diseases in humans. However, inconsistency and ambiguity instructions show a lack of definitive knowledge on best spray use techniques. In this study, we have identified new usage strategy nasal available over-the-counter, that registers an average 8-fold improvement topical delivery drugs at diseased sites, when compared to prevalent The protocol involves re-orienting axis harness...
BackgroundMany individuals with cystic fibrosis (CF) have chronic rhinosinusitis resulting in nasal obstruction, sinus infections, and repeated surgeries. Elexacaftor-tezacaftor-ivacaftor is a highly effective modulator therapy approved for aged 6 years or older CF who at least one F508del allele other responsive mutation. The current study tests the hypothesis that ELX/TEZ/IVA improves sinonasal disease CF.MethodsThe was pre/post, observational cohort conducted two sites. Participants...
p62/Sequestosome 1 is a scaffold protein involved in the regulation of autophagy, trafficking proteins to proteasome, and activation NF-kappaB. p62 encodes an N-terminal PB1 domain addition ZZ domain, TRAF6-binding LC3 interaction region, ubiquitin-associated each critical for physiological function p62. domains have beta-grasp topology where front end one binds back second domain. The homodimerizes as well heterodimerizes with other domains. atypical kinases C, MAPK kinase, MEK5, NBR1...
G-protein heterotrimers, composed of a guanine nucleotide-binding Gα subunit and an obligate Gβγ dimer, regulate signal transduction pathways by cycling between GDP- GTP-bound states. Signal deactivation is achieved Gα-mediated GTP hydrolysis (GTPase activity) which enhanced the GTPase-accelerating protein (GAP) activity “regulator signaling” (RGS) proteins. In cellular context, RGS proteins have also been shown to speed up onset signaling, accelerate without changing amplitude or...
Abstract Objectives/Hypothesis: The nasoseptal flap provides hearty vascularized tissue for reconstruction of expanded endonasal approaches (EEA); however, it produces donor site morbidity due to exposed cartilage. Mucosalization the septum requires 12 weeks, multiple debridements, and frequent saline rinses. This study addresses reduction nasal by grafting middle turbinate mucosa onto septum. Study Design: Small prospective with retrospective analysis a larger cohort. Methods: Fifteen...
"Regulator of G-protein signaling" (RGS) proteins facilitate the termination G protein-coupled receptor (GPCR) signaling via their ability to increase intrinsic GTP hydrolysis rate Galpha subunits (known as GTPase-accelerating protein or "GAP" activity). RGS2 is unique in its vitro potency and selectivity a GAP for Galpha(q) subunits. As many vasoconstrictive hormones signal G(q) heterotrimer-coupled receptors, it perhaps not surprising that RGS2-deficient mice exhibit constitutive...
ABSTRACT Despite signs of infection, the involvement oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, 15 immune cell clusters. Analysis SARS-CoV-2 viral entry factor expression showed enrichment epithelia including ducts acini suprabasal cells mucosae. autopsy tissues confirmed vivo infection mucosa. Saliva SARS-CoV-2-infected individuals...
Objectives/Hypothesis Information on polymorphous low-grade adenocarcinoma (PLGA) consists primarily of case reports and small institutional series with varying recurrence rates. In this report, we describe our experience conduct a review the literature to assess overall incidence PLGA among oral salivary gland tumors determine Study Design A retrospective literature-based was performed. Methods Retrospective at an academic tertiary referral center. Review clinical records pathological...
Objectives/Hypothesis Historically, narrative letters of recommendation have been utilized in the selection applicants for otolaryngology residency programs. In last two application cycles, our specialty adopted a standardized letter (SLOR). The intent was to decrease time burden writers and provide readers with an objective evaluation applicants. this study determine attributes SLOR that correlate matching into program. Study Design We performed retrospective using SLOR, United States...
The vast majority of people with cystic fibrosis (CF) are now eligible for CF transmembrane regulator (CFTR) modulator therapy. remaining individuals harbor premature termination codons (PTCs) or rare CFTR variants limited treatment options. Although the clinical response can be reliably predicted using primary airway epithelial cells, cells carrying scarce. To overcome this obstacle, cell lines created by overexpression mouse Bmi-1 and human TERT (hTERT). Using approach, we developed 2...
Correlation between symptom-based surveys and objective olfactory testing is variable. For diagnosis symptom monitoring, should correlate with testing. The Odor Awareness Scale (OAS) Affective Importance of (AIO) showed significant but moderate positive correlations University Pennsylvania Scent Identification Test (UPSIT) score.
Objectives The 22‐question SinoNasal Outcome Test (SNOT‐22) assesses chronic rhinosinusitis (CRS) severity. We aimed to identify predictors of SNOT‐22 score improvement following highly effective modulator therapy (HEMT) initiation and corroborate the minimal clinically important difference (MCID) in adults with cystic fibrosis (CF). Methods Prospective observational data was pooled from four studies across 10 US centers investigating people CF (PwCF) CRS. Three evaluated HEMT's impact on...
Simulators allow junior otolaryngology residents to practice the delicate procedure of pressure equalization tube (PET) insertion. However, most simulators lack ability mimic differing anatomic complexities between patients, such as variable external auditory canal (EAC) size. We developed a novel low-cost, medium-fidelity 3-dimensional-printed PET simulator with different EAC sizes better reflect complexity. Additionally, variety materials were tested elastic modulus tympanic membrane,...