A5048384124- Spaceflight effects on biology
- Genomics, phytochemicals, and oxidative stress
- Nutrition, Genetics, and Disease
- Glutathione Transferases and Polymorphisms
- Effects of Radiation Exposure
- MicroRNA in disease regulation
- Mathematical Biology Tumor Growth
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- BRCA gene mutations in cancer
- Bioinformatics and Genomic Networks
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- Lymphoma Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Gene expression and cancer classification
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Veterinary Oncology Research
Georgetown University
2020-2024
Broward Health
2024
SRI International
2024
University of Florida
1991-2023
The Ohio State University Wexner Medical Center
2023
Howard University
2023
Howard University Hospital
2023
Hampton University
2014-2022
Cancer Research Center
2015-2022
Georgetown University Medical Center
2020-2021
Highlights•In silico predictions of miR-2392 as a miRNA involved with SARS-CoV-2•Overexpression produces similar biological response COVID-19 infection•miR-2392 is confirmed to circulate in serum and urine patients COVID-19•Development initiated potential antiviral therapeutic against COVID-19SummaryMicroRNAs (miRNAs) are small non-coding RNAs post-transcriptional gene regulation that have major impact on many diseases provide an exciting avenue toward therapeutics. From patient...
We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents humans in response to simulated, short-duration long-duration spaceflight. Previous studies miRNAs regulate rodent responses spaceflight low-Earth orbit, we confirmed the expression of these proposed reacting simulated conditions. Moreover, astronaut samples from NASA Twins Study signatures miRNA sequencing, single-cell RNA sequencing (scRNA-seq), assay for transposase accessible...
Abstract Our previous research revealed a key microRNA signature that is associated with spaceflight can be used as biomarker and to develop countermeasure treatments mitigate the damage caused by space radiation. Here, we expand on this work determine biological factors rescued treatment. We performed RNA-sequencing transcriptomic analysis 3D microvessel cell cultures exposed simulated deep radiation (0.5 Gy of Galactic Cosmic Radiation) without antagonists three microRNAs: miR-16-5p,...
With the development of transcriptomic technologies, we are able to quantify precise changes in gene expression profiles from astronauts and other organisms exposed spaceflight. Members NASA GeneLab GeneLab-associated analysis working groups (AWGs) have developed a consensus pipeline for analyzing short-read RNA-sequencing data spaceflight-associated experiments. The includes quality control, read trimming, mapping, quantification steps, culminating detection differentially expressed genes....
Abstract Background Spaceflight poses a unique set of challenges to humans and the hostile spaceflight environment can induce wide range increased health risks, including dermatological issues. The biology driving frequency skin issues in astronauts is currently not well understood. Methods To address this issue, we used systems approach utilizing NASA’s Open Science Data Repository (OSDR) on space flown murine transcriptomic datasets focused skin, biochemical profiles 50 NASA human...
Abstract There remains a need to identify new sensitive diagnostic and predictive blood-based platforms in lymphoma. We previously discovered novel circulating microRNA (miRNA) signature Smurf2-deficient mouse model that spontaneously develops diffuse large B-cell lymphoma (DLBCL). Herein, we investigated this 10-miRNA (miR-15a, let-7c, let-7b, miR-27a, miR-10b, miR-18a, miR-497, miR-130a, miR24, miR-155) human cell lines, mice engrafted with patient-derived xenografts (PDXs), DLBCL patient...
Rationale: Viral infections are complex processes based on an intricate network of molecular interactions. The infectious agent hijacks components the cellular machinery for its profit, circumventing natural defense mechanisms triggered by infected cell. successful completion replicative viral cycle within a cell depends function versus defenses. Non-coding RNAs (ncRNAs) important modulators, either promoting or preventing progression infections. Among these ncRNAs, long non-coding RNA...
Abstract Aging is the major determinant of cancer incidence, which, in turn, likely dictated large part by processes that influence progression early subclinical (occult) cancers. However, there little understanding how aging informs changes aggregate host signaling favor progression. In this study, we provide direct evidence can serve as an organizing axis to define progression-modulating processes. As a model system explore concept, employed adolescent (68 days), young adult (143...
Pelvic radiation for cancer therapy can damage a variety of normal tissues. In this study, we demonstrate that causes acute changes to pelvic fibroblasts such as the transformation myofibroblasts and induction senescence, which persist months after radiation. The addition manganese porphyrin, MnTE-2-PyP, resulted in protection these persisted following exposure. Specifically, at two post-radiation, MnTE-2-PyP inhibited number α-smooth muscle actin positive induced by six significantly...
Space radiation has recently been considered a risk factor for astronauts’ cardiac health. As an example, the case of how to query and identify datasets within NASA’s GeneLab database demonstrate utility, we used unbiased systems biology method identifying key genes/drivers contribution space on cardiovascular system. This knowledge can contribute designing appropriate experiments targeting these specific pathways. Microarray data from cardiomyocytes male C57BL/6 mice followed-up 28 days...
<p class="Pa7"><strong>Background: </strong>Variants of unknown significance (VUSs) have been identified in <em>BRCA1 </em>and <em>BRCA2 account for the majority all sequence alterations. Notably, VUSs occur disproportionately people African descent hampering breast cancer (BCa) management and prevention efforts population. Our study sought to identify characterize mutations associated with increased risk BCa at young age.</p><p...
The concept of age-dependent host control cancer development raises the natural question how these effects manifest across tissue/organ types with which a tumor interacts, one important component is aging immune system. To investigate this, changes in spleen, an nexus mouse, was examined for its interactive influence on carcinogenesis process. model C57BL/6 male mice (adolescent, young adult, middle-aged, and old or 68, 143, 551 736 days respectively) without syngeneic murine implant....
Background: Ionizing radiation from galactic cosmic rays (GCR) is one of the major risk factors that will impact health astronauts on extended missions outside protective effects Earth’s magnetic field. The NASA GeneLab project has detailed information exposure using animal models with curated dosimetry for spaceflight experiments. Methods: We analyzed multiple omics datasets associated both ground-based and studies included in vivo vitro approaches. A range ions protons to iron particles...
// J. Tyson McDonald 1, * , Athena Kritharis 2, Afshin Beheshti 3 Monika Pilichowska 4 Kristine Burgess 5 Luisel Ricks-Santi 1 Elizabeth McNiel Cheryl A. London Dashnamoorthy Ravi 2 and Andrew M. Evens Cancer Research Center, Hampton University, Hampton, VA, USA Division of Blood Disorders, Rutgers Institute New Jersey, Brunswick, NJ, WYLE, NASA Ames Moffett Field, CA, Pathology, Tufts Medical Boston, MA, Cummings School Veterinary Medicine, These authors contributed equally to this work...
Potential molecular alterations based on age and sex are not well defined in diffuse large B-cell lymphoma (DLBCL). We examined global transcriptome DLBCL data from The Cancer Genome Atlas (TCGA) via a systems biology approach to determine the differences associated with sex. Collectively, revealed striking transcriptional older decreased metabolism telomere functions female was interferon signaling, transcription, cell cycle, PD-1 signaling. discovered that key genes for most groups...
Extensive epidemiological data have demonstrated an exponential rise in the incidence of non-Hodgkin lymphoma (NHL) that is associated with increasing age. The molecular etiology this remains largely unknown, which impacts effectiveness treatment for patients. We proposed age-dependent circulating microRNA (miRNA) signatures host influence diffuse large B cell (DLBCL) development. Our objective was to examine tumor development age-based DLBCL system using inventive systems biology approach....
Radiation is a common anticancer therapy for prostate cancer, which transforms tumor-associated normal fibroblasts to myofibroblasts, resulting in fibrosis. Oxidative stress caused by radiation-mediated mitochondrial damage one of the major contributors As diabetics are oxidatively stressed, reactive oxygen species cause severe treatment failure, treatment-related side effects, and significantly reduced survival diabetic cancer patients as compared non-diabetic patients. Hyperglycemia...
Enrichment of cancer stem cells (CSCs) is thought to be responsible for glioblastoma multiforme (GBM) recurrence after radiation therapy. Simulation results from our agent-based cellular automata model reveal that the enrichment CSCs may result either an increased symmetric self-renewal division rate or a reprogramming non-stem (CCs) cell state. Based on plateau-to-peak ratio CSC fraction in tumor following radiation, downward trend peak subsequent plateau (i.e., exceeding 1.0) was found...
Spaceflight poses a unique set of challenges to humans and the hostile environment can induce wide range increased health risks, including dermatological issues. The biology driving frequency skin issues in astronauts is currently not well understood. To address this issue, we used systems approach utilizing NASA's Open Science Data Repository (OSDR) on spaceflown murine transcriptomic datasets focused skin, biomedical profiles from fifty NASA astronauts, confirmation via data JAXA Twins...