A5005084000- Spaceflight effects on biology
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Systemic Sclerosis and Related Diseases
- Biomedical Text Mining and Ontologies
- HER2/EGFR in Cancer Research
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Health, Environment, Cognitive Aging
- High Altitude and Hypoxia
- RNA modifications and cancer
- Breast Cancer Treatment Studies
- Genetic Syndromes and Imprinting
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Semantic Web and Ontologies
- MicroRNA in disease regulation
- Effects and risks of endocrine disrupting chemicals
- Effects of Radiation Exposure
- Mycotoxins in Agriculture and Food
- Microbial Community Ecology and Physiology
- Space Science and Extraterrestrial Life
- Dermatologic Treatments and Research
- Muscle Physiology and Disorders
University of Salford
2025
University of Staffordshire
2021-2024
International Space University
2022-2024
Queen's University Belfast
2019-2023
University of Malta
2022
Medical University of South Carolina
2017-2019
Universidade de São Paulo
2013-2018
Clinics Hospital of Ribeirão Preto
2015-2018
University of Charleston
2017
Universidade de Ribeirão Preto
2013-2014
Mitral valve prolapse (MVP) affects 1 in 40 people and is the most common indication for mitral surgery. MVP can cause arrhythmias, heart failure, sudden cardiac death, to date, causes of this disease are poorly understood. We now demonstrate that defects primary cilia genes their regulated pathways familial sporadic nonsyndromic cases. Our expression studies genetic ablation experiments confirmed a role regulating ECM deposition during development. Loss development resulted progressive...
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
Abstract Spaceflight has several detrimental effects on the physiology of astronauts, many which are recapitulated in rodent models. Mouse studies performed Space Shuttle showed disruption lipid metabolism liver. However, given that these animals were not sacrificed on-orbit and instead returned live to earth, it is unclear if disruptions solely induced by space stressors (e.g. microgravity, radiation) or part explained stress return Earth. In this work we analyzed three liver datasets from...
Abstract Extended spaceflight has been shown to adversely affect astronaut visual acuity. The purpose of this study was determine whether alters gene expression profiles and induces oxidative damage in the retina. Ten week old adult C57BL/6 male mice were flown aboard ISS for 35 days returned Earth alive. Ground control maintained on under identical environmental conditions. Within 38 (+/−4) hours after splashdown, ocular tissues collected analysis. RNA sequencing detected 600 differentially...
The organic compound diethylhexyl phthalate (DEHP) represents a high production volume chemical found in cosmetics, personal care products, laundry detergents, and household items. DEHP, along with other phthalates causes endocrine disruption males. Exposure to disrupting chemicals has been linked the development of several adverse health outcomes apical end points including Non-Alcoholic Fatty Liver Disease (NAFLD). This study examined adult male zebrafish (Danio rerio) transcriptome after...
Scleroderma-associated pulmonary fibrosis (SSc-PF) and idiopathic (IPF) are two of many chronic fibroproliferative diseases that responsible for nearly 45% all deaths in developed countries. While sharing several pathobiological characteristics, they also have very distinct features. Currently no effective antifibrotic treatments exist can halt the progression PF or reverse it. Our goal is to uncover potential gene targets development therapies efficacious both diseases, those specific...
With the development of transcriptomic technologies, we are able to quantify precise changes in gene expression profiles from astronauts and other organisms exposed spaceflight. Members NASA GeneLab GeneLab-associated analysis working groups (AWGs) have developed a consensus pipeline for analyzing short-read RNA-sequencing data spaceflight-associated experiments. The includes quality control, read trimming, mapping, quantification steps, culminating detection differentially expressed genes....
Abstract Incidence of kidney stones in astronauts is a major risk factor associated with long-term missions, caused by increased blood calcium levels due to bone demineralisation triggered microgravity and space radiation. Transcriptomic changes have been observed tissues during spaceflight, including the kidney. We analysed transcriptome patterns two different strains mice flown on International Space Station, C57BL/6J BALB/c. Here we show link between spaceflight dysregulation lipid...
Breast cancer is the most common in women worldwide and metastatic dissemination principal factor related to death by this disease. stem cells (bCSC) are thought be responsible for metastasis chemoresistance. In study, based on whole transcriptome analysis from putative bCSC reverse engineering of transcription control networks, we identified two networks associated with phenotype. One controlled SNAI2, TWIST1, BNC2, PRRX1 TBX5 drives a mesenchymal or CSC-like The second network SCML4,...
Systemic sclerosis (SSc) is a complex disease of unknown aetiology in which inflammation and fibrosis lead to multiple organ damage. There currently no effective therapy that can halt the progression or reverse it, thus studies provide novel insights into pathogenesis identify potential therapeutic targets are critically needed.We used global gene expression genome-wide DNA methylation analyses dermal fibroblasts (dFBs) from unique cohort twins discordant for SSc molecular features this...
Ubiquitous exposure to bisphenol A (BPA), an endocrine disruptor (ED), has raised concerns for both human and ecosystem health. Epigenetic factors, including microRNAs (miRNAs), are key regulators of gene expression during cancer. The effect BPA on the zebrafish epigenome remains poorly characterized. Zebrafish represents excellent model study cancer as organism develops a disease that resembles Using systems toxicology model, we hypothesized chronic BPA-exposure impacts miRNome in adult...
It is estimated that 30% of all genes in the mammalian cells are regulated by microRNA (miRNAs). The most relevant miRNAs a cellular context not necessarily those with greatest change expression levels between healthy and diseased tissue. Differentially expressed (DE) modulate large number messenger RNA (mRNA) transcripts ultimately have greater influence determining phenotypic outcomes more important global biological than just few mRNA transcripts. Here, we describe development tool,...
Up to 30-40% of Ewing sarcoma (EwS) patients with non-metastatic disease develop local or metastatic relapse within a time span 2-10 years. This is in part caused by the absence prognostic biomarkers that can identify high-risk and thus assign them risk-adapted monitoring treatment regimens. Since cancer stemness has been associated tumour poor patient outcomes, we investigated current study potential SOX2 (sex determining region Y box 2) - major transcription factor involved development...
Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following combination analog conditions using mouse model: simulated microgravity via hindlimb unloading (HLU) and/or low-dose γ-ray irradiation (LDR) 21 days, followed by 4 months readaptation. Changes in gene expression epigenetic modifications brain samples during readaptation...
Human expansion in space is hampered by the physiological risks of spaceflight. The muscle and liver are among most affected tissues during spaceflight their relationships response to exposure have never been studied. We compared transcriptome quadriceps from mice on NASA RR1 mission, after 37 days using GSEA, ORA, sparse partial least square-differential analysis. found that lipid metabolism biological process between two organs. A specific gene cluster expression pattern strongly...
Cellular senescence is a state of permanent growth arrest that arises once cells reach the limit their proliferative capacity. It creates an inflammatory microenvironment favouring initiation and progression various age-related diseases, including prostate cancer. Non-coding RNAs (ncRNAs) have emerged as important regulators cellular gene expression. Nonetheless, very little known about interplay microRNAs (miRNAs) long non-coding (lncRNAs) how deregulation ncRNA networks promotes...
Systemic sclerosis (SSc) is a complex multi-system autoimmune disease characterized by immune dysregulation, vasculopathy, and organ fibrosis. Skin fibrosis causes high morbidity impaired quality of life in affected individuals. Animal models do not fully recapitulate the human disease. Thus, there critical need to identify ex vivo for dermal characteristic SSc. We identified genes regulated pro-fibrotic factor TGFβ skin maintained culture. The molecular signature overlapped with that which...
Background: Ionizing radiation from galactic cosmic rays (GCR) is one of the major risk factors that will impact health astronauts on extended missions outside protective effects Earth’s magnetic field. The NASA GeneLab project has detailed information exposure using animal models with curated dosimetry for spaceflight experiments. Methods: We analyzed multiple omics datasets associated both ground-based and studies included in vivo vitro approaches. A range ions protons to iron particles...
Bioinformatics approaches have proven useful in understanding biological responses to spaceflight. Spaceflight experiments remain resource intensive and rare. One outstanding issue is how maximize scientific output from a limited number of omics datasets traditional animal models including nematodes, fruitfly, rodents. The utility data invertebrate anticipating mammalian spaceflight has not been fully explored. Hence, we performed comparative analyses transcriptomes soleus extensor digitorum...
The etiology and reasons underlying the ethnic disparities in systemic sclerosis (SSc) remain unknown. African Americans are disproportionally affected by SSc yet underrepresented research. aim of this study was to comprehensively investigate association DNA methylation levels with dermal fibroblasts from patients ancestry. Reduced representation bisulfite sequencing (RRBS) performed on primary 15 controls ancestry, over 3.8 million CpG sites were tested for differential patterns between...
Abstract Mankind’s quest for a manned mission to Mars is placing increased emphasis on the development of innovative radio-protective countermeasures long-term space travel. Hibernation confers effects in hibernating animals, and this has led investigation synthetic torpor mitigate deleterious chronic low-dose-rate radiation exposure. Here we describe an induced model developed using zebrafish. We explored exposure with focus liver. Transcriptomic behavioural analyses were performed....
The purpose of this study was to retrospectively review the pathologic complete response (pCR) rate from patients (n=86) with stage II and III HER2-positive breast cancer treated neoadjuvant chemotherapy at our institution 2008 2013 determine possible predictive prognostic factors. Immunohistochemistry for hormone receptors Ki-67 carried out. Clinical pathological features were analyzed as factors therapy. For survival analysis, we used Kaplan-Meier curves estimate 5-year rates log-rank test...
The existence of tumor-initiating cells (TICs) within solid tumors has been hypothesized to explain tumor heterogeneity and resistance cancer therapy. In breast cancer, the expression CD44 CD24 activity aldehyde dehydrogenase 1 (ALDH1) can be used selectively isolate a cell population enriched in TICs. However, ideal marker identify TICs not established. aim this study was evaluate novel potential markers for TIC carcinoma. We prospectively analyzed CD44, CD24, ABCG2, CXCR4, ALDH1 by using...