Kevin Nestler
A5074165375- Spaceflight effects on biology
- RNA modifications and cancer
- Chromatin Remodeling and Cancer
- Advanced Proteomics Techniques and Applications
- Acute Myeloid Leukemia Research
- Protein Degradation and Inhibitors
- Insurance, Mortality, Demography, Risk Management
- Epigenetics and DNA Methylation
- Climate Change and Health Impacts
- Space Science and Extraterrestrial Life
- Multiple Myeloma Research and Treatments
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Genetics, Aging, and Longevity in Model Organisms
- Circular RNAs in diseases
- Advanced Biosensing Techniques and Applications
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Neuroethics, Human Enhancement, Biomedical Innovations
- Mass Spectrometry Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Biosensors and Analytical Detection
- Genomics and Chromatin Dynamics
George Washington University
2023-2024
Children's Hospital of Philadelphia
2019-2023
University of Pennsylvania
2021
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
Abstract Ovarian cancers (OC) are characterized by an immunosuppressive tumor microenvironment and frequent mutations in the TP53 gene, which encodes for p53 suppressor protein. Low infiltration of tumor-fighting immune cells these tumors contribute to ovarian cancer’s static 5-year survival rate poor response immunotherapy treatment. We others have shown that using therapies inhibit DNA methylation (DNMTis) can facilitate cell recruitment activation fight cancers. This is mediated...
With the rapid developments in mass spectrometry (MS)-based proteomics methods, label-free semiquantitative has become an increasingly popular tool for profiling global protein abundances unbiased manner. However, reproducibility of these data across time and LC–MS platforms is not well characterized. Here, we evaluate performance three (Orbitrap Elite, Q Exactive HF, Orbitrap Fusion) analysis cell surface proteins over a six-year period. Sucrose gradient ultracentrifugation was used...
Background: Pediatric acute myeloid leukemia (AML) is the second most common pediatric but accounts for about 50% of leukemia-related mortality. While transcriptomics has identified novel AML subgroups, therapeutic targets and potential biologic mechanisms underlying treatment failure, only one proteomic array study evaluated subsets AML. Thus, to our knowledge, a comprehensive, integrated proteogenomic analysis lacking. Such an could identify new drivers failure. Using 182 matched...
<title>Abstract</title> Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights)...
Abstract Acute myeloid leukemia (AML) therapy requires very intensive chemotherapy/stem cell transplant that cures only 60% of children and 25% adults with AML. The development chimeric antigen receptor (CAR) T cells for AML has been hampered by the lack optimal surface antigens. We hypothesized unbiased identification proteins will enable creation novel CAR immunotherapies integration surfaceome data RNA DNA sequencing facilitate new therapeutic targets. also pediatric adult surfaceomes are...