Han-Chung Lee
A5085191164- Spaceflight effects on biology
- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Legume Nitrogen Fixing Symbiosis
- Renal and related cancers
- Methane Hydrates and Related Phenomena
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Vibrio bacteria research studies
- Renal Diseases and Glomerulopathies
- Neuroethics, Human Enhancement, Biomedical Innovations
- Algal biology and biofuel production
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Nematode management and characterization studies
- Nutritional Studies and Diet
- Birth, Development, and Health
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Chronic Kidney Disease and Diabetes
- Plant nutrient uptake and metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Proteomics Techniques and Applications
- Gut microbiota and health
Monash University
2023-2024
Australian Regenerative Medicine Institute
2023-2024
Australian National University
2021-2023
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
Legumes acquire soil nutrients through nitrogen-fixing root nodules and lateral roots. To balance the costs benefits of nodulation, legumes negatively control nodule number by autoregulatory hormonal pathways. How simultaneously coordinate development to procure remains poorly understood. In Medicago (Medicago truncatula), a subset mature C-TERMINALLY ENCODED PEPTIDE (CEP) hormones can systemically promote number, but all CEP tested date regulate number. Here we showed that CEP7 produces...
Abstract Diverse aerobic bacteria use atmospheric hydrogen (H 2 ) and carbon monoxide (CO) as energy sources to support growth survival. Such trace gas oxidation is recognised a globally significant process that serves the main sink in biogeochemical H cycle sustains microbial biodiversity oligotrophic ecosystems. However, it unclear whether archaea can also . Here we show thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes CO sub-atmospheric levels....
Filamentous algae (FA) have potential advantages over microalgae for wastewater treatment. However, their implementation at a large-scale is hindered by an inability to predict performance. This study compared the cellular responses (photosynthesis and respiration) composition (pigments photosystem proteins) of FA Oedogonium acclimatised average summer winter conditions (Melbourne, Australia). After seven days acclimation Chl content 'summer acclimated' (SA) was about half that 'winter (WA)...
The type VI secretion system (T6SS) is a molecular machine utilised by many Gram-negative bacteria to deliver antibacterial toxins into adjacent cells. Here we present the structure of Tse15, T6SS Rhs effector from nosocomial pathogen Acinetobacter baumannii. Tse15 forms triple layered β-cocoon domain with an N-terminal α-helical clade and unfolded C-terminal toxin inside cage. cleaved three domains, through independent auto-cleavage events involving aspartyl protease activity for...
The gut microbiota is a crucial link between diet and cardiovascular disease (CVD). Using fecal metaproteomics, method that concurrently captures human microbiome proteins, we determined the crosstalk microbiome, diet, health, CVD. Traditional CVD risk factors (age, BMI, sex, blood pressure) explained < 10% of proteome variance. However, unsupervised protein-based clustering analysis revealed two distinct clusters (low-risk high-risk) with different pressure (by 9 mmHg) sex-dependent dietary...
C-TERMINALLY ENCODED PEPTIDEs (CEPs) control diverse responses in plants including root development, system architecture, nitrogen demand signalling, and nutrient allocation that influences yield, there is evidence different ligands impart phenotypic responses. Thus, a need for simple method identifies bona fide CEP hormone-receptor pairings vivo examines whether family peptides bind the same receptor. We used formaldehyde or photoactivation to cross-link fluorescently tagged group 1 2 CEPs...
Abstract In soil ecosystems, obligately aerobic bacteria survive oxygen deprivation (hypoxia) by entering non-replicative persistent states. Little is known about how these rewire their metabolism to stay viable in The model obligate aerobe Mycobacterium smegmatis maintains redox homeostasis during hypoxia mediating fermentative hydrogen production. However, the fate of organic carbon fermentation, and associated remodeling metabolism, unresolved. Here we systematically profiled M. growth,...
Background/Objective: The gut microbiota is a crucial link between diet and cardiovascular health by producing beneficial metabolites, such as short-chain fatty acids (SCFAs), through fibre fermentation. However, most studies have focused on microbial DNA to infer function. Here, we employed metaproteomics, novel method capture human protein expression profiles concurrently. We aimed investigate the crosstalk microbiota, diet, host health, blood pressure (BP). Methods: Mass...
ABSTRACT Acute kidney injury (AKI) is a common clinical disorder linked to high rates of illness and death. Ischemia leading cause AKI, which can result in chronic disease (CKD) through maladaptive repair process characterised by failed epithelial regeneration, inflammation, metabolic dysregulation. No targeted therapies exist prevent the AKI CKD transition insight into ischemic humans remains limited. In this study, we report that human organoids recapitulate select molecular signatures...
<title>Abstract</title> Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights)...