A5041043165- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Metalloenzymes and iron-sulfur proteins
- ATP Synthase and ATPases Research
- Electrocatalysts for Energy Conversion
- Ubiquitin and proteasome pathways
- Heme Oxygenase-1 and Carbon Monoxide
- Ammonia Synthesis and Nitrogen Reduction
- Porphyrin Metabolism and Disorders
- Hemoglobin structure and function
- Chemical Reactions and Isotopes
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
- Advanced Power Generation Technologies
- Bacterial biofilms and quorum sensing
- Hybrid Renewable Energy Systems
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- Antibiotic Resistance in Bacteria
- Endoplasmic Reticulum Stress and Disease
- Hydrogen Storage and Materials
- Protein Kinase Regulation and GTPase Signaling
- Protein Structure and Dynamics
Australian Regenerative Medicine Institute
2021-2025
Monash University
2021-2025
Discovery Institute
2024
The University of Melbourne
2016-2023
Walter and Eliza Hall Institute of Medical Research
2016-2023
Abstract Diverse aerobic bacteria use atmospheric H 2 as an energy source for growth and survival 1 . This globally significant process regulates the composition of atmosphere, enhances soil biodiversity drives primary production in extreme environments 2,3 Atmospheric oxidation is attributed to uncharacterized members [NiFe] hydrogenase superfamily 4,5 However, it remains unresolved how these enzymes overcome extraordinary catalytic challenge oxidizing picomolar levels amid ambient poison O...
We present an in-depth analysis of selected CASP15 targets, focusing on their biological and functional significance. The authors the structures identify discuss key protein features evaluate how effectively these aspects were captured in submitted predictions. While overall ability to predict three-dimensional continues impress, reproducing uncommon not previously observed experimental is still a challenge. Furthermore, instances with conformational flexibility large multimeric complexes...
The mammalian pseudokinase SgK223, and its structurally related homologue SgK269, are oncogenic scaffolds that nucleate the assembly of specific signalling complexes regulate tyrosine kinase signalling. Both SgK223 SgK269 form homo- hetero-oligomers, a mechanism underpins diversity outputs. However, mechanistic insights into heterotypic association lacking. Here we present crystal structure domain adjacent N- C-terminal helices. reveals how C-regulatory helices engage in novel fold to...
Abstract PEAK pseudokinases regulate cell migration, invasion and proliferation by recruiting key signaling proteins to the cytoskeleton. Despite lacking catalytic activity, alteration in their expression level is associated with several aggressive cancers. Here, we elucidate molecular details of interactions adapter CrkII Grb2 scaffold protein 14-3-3. Our findings rationalize why dimerization has a crucial function signal transduction provide biophysical structural data unravel binding...
Abstract Diverse aerobic bacteria use atmospheric hydrogen (H 2 ) and carbon monoxide (CO) as energy sources to support growth survival. Such trace gas oxidation is recognised a globally significant process that serves the main sink in biogeochemical H cycle sustains microbial biodiversity oligotrophic ecosystems. However, it unclear whether archaea can also . Here we show thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes CO sub-atmospheric levels....
Diverse bacteria and archaea use atmospheric CO as an energy source for long-term survival. Bacteria [MoCu]-CO dehydrogenases (Mo-CODH) to convert carbon dioxide, transferring the obtained electrons aerobic respiratory chain. However, it is unknown how these enzymes oxidize at low concentrations interact with Here, we cryo-electron microscopy structural modeling show Mo-CODHMs (CoxSML) from Mycobacterium smegmatis interacts its partner, membrane-bound menaquinone-binding protein CoxG. We...
All cells require a continuous supply of the universal energy currency, adenosine triphosphate (ATP), to drive countless cellular reactions. The universally conserved F1Fo-ATP synthase regenerates ATP from ADP and Pi by harnessing transmembrane electrochemical proton gradient (pmf). Bacteria have evolved wide diversity pmf-forming proteins make using light, organic, inorganic sources. Recently, we proposed that many bacteria survive atmospheric trace gases produce when limited for other...
Abstract Doublecortin-like kinase 1 (DCLK1) is an understudied bi-functional with a proven role in tumour growth and development. However, the presence of tissue-specific spliced DCLK1 isoforms distinct biological functions have challenged development effective strategies to understand oncogenesis. Recently, DCLK1-IN-1 was reported as highly selective inhibitor, powerful tool dissect functions. Here, we report crystal structures domain complex its precursors. Combined, our data rationalises...
Carbon monoxide has an infamous reputation as a toxic gas, and it been suggested that potential antibacterial agent. Despite this, how bacteria resist its effects is not well understood.
Abstract Diverse bacteria and archaea use atmospheric carbon monoxide (CO) as an energy source during long-term survival. This process enhances the biodiversity of soil marine ecosystems globally removes 250 million tonnes a toxic, climate-relevant pollutant from atmosphere each year. Bacteria [MoCu]-carbon dehydrogenases (Mo-CODH) to convert CO dioxide, then transfer liberated high-energy electrons aerobic respiratory chain. However, given no high-affinity Mo-CODH has been purified, it is...
Acinetobacter baumannii is a high-risk pathogen due to the rapid global spread of multidrug-resistant lineages. Its phylogenetic divergence from other ESKAPE pathogens means that determinants its antimicrobial resistance can be difficult extrapolate widely studied bacteria. A recent study showed A. upregulates production an outer membrane lipoprotein, which we designate BonA, in response challenge with polymyxins. Here, show BonA has limited sequence similarity and distinct structural...
Iron is an essential nutrient for most bacteria and often growth-limiting during infection, due to the host sequestering free iron as part of innate immune response. To obtain required growth, many bacterial pathogens encode transporters capable extracting iron-containing cofactor heme directly from proteins. Pathogenic E. coli Shigella spp. produce outer membrane transporter ChuA, which binds hemoglobin extracts its cofactor, before importing into cell. Heme extraction by ChuA a dynamic...
Abstract Doublecortin-like kinase 1 (DCLK1) is a bi-functional protein classified as Microtubule-Associated Protein (MAP) and serine/threonine that plays critical role in regulating microtubule assembly. This understudied upregulated or mutated wide range of cancers. Knockdown studies have shown DCLK1 functionally important for tumour growth. However, the presence tissue development specific spliced isoforms lack systematic evaluation their biological function challenged effective strategies...
Abstract PEAK pseudokinases regulate cell migration, invasion and proliferation by recruiting key signaling proteins to the cytoskeleton. Despite lacking catalytic activity, alteration in their expression level is associated with several aggressive cancers. Here, we elucidate new molecular details of interactions adapter CrkII Grb2 scaffold protein 14-3-3. Our findings rationalize why dimerization has a crucial function signal transduction provide biophysical structural data unravel binding...
Abstract Diverse aerobic bacteria use atmospheric H 2 as an energy source for growth and survival. This recently discovered yet globally significant process regulates the composition of atmosphere, enhances soil biodiversity, drives primary production in certain extreme environments. Atmospheric oxidation has been attributed to still uncharacterised members [NiFe]-hydrogenase superfamily. However, it is unresolved how these enzymes overcome extraordinary catalytic challenge selectively...
Many soil bacteria lack the organic compounds required for growth and, as a result, exist in dormant non-replicative state.In this state, still need low level of energy cell maintenance and to maintain proton motive force.Bacteria oxidise reduced trace gases atmosphere obtain energy, most notably hydrogen (H2) carbon monoxide (CO) [1].The potential electrons from process enter cellular respiratory chain, contributing force ATP generation.While these are present at extremely levels (530 parts...
PEAK pseudokinases (PEAK1, PEAK2 and PEAK3) are important protein scaffolds that regulate cell migration, invasion proliferation by recruiting signalling effectors at the cytoskeleton [1,2].Despite lacking catalytic activity, alteration in expression level of family members is associated with several type aggressive cancers.An emerging thread function ability to utilise homo-and hetero-dimerisation dynamically integrate diversify cellular signals.Our lab was first structurally reveal a...
Mycobacterium smegmatis is a heterotrophic, obligately aerobic soil dwelling bacterium that requires organic carbon for growth.Due to fierce competition resources in soil, the often experiences deprivation of both oxygen and sources.To cope with this, M. has evolved persist through metabolic flexibility, upregulating enzymes, such as hydrogenase Huc, enable it utilise electron donors acceptors other than molecular oxygen.Huc provides electrons from H2 cytochrome bcc-aa3 oxidase supercomplex...
Abstract PEAK pseudokinases regulate cell migration, invasion and proliferation by recruiting key signaling proteins to the cytoskeleton. Despite lacking catalytic activity, alteration in their expression level is associated with several aggressive cancers. Here, we elucidate new molecular details of interactions adapter CrkII Grb2 scaffold protein 14-3-3. Our findings rationalize why dimerization has a crucial function signal transduction provide biophysical structural data unravel binding...