A5010453425- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- High-Temperature Coating Behaviors
- Microstructure and Mechanical Properties of Steels
- Bacterial biofilms and quorum sensing
- Bacillus and Francisella bacterial research
- Biochemical and Molecular Research
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Antibiotic Resistance in Bacteria
- Conducting polymers and applications
- Advanced oxidation water treatment
- Vibrio bacteria research studies
- Iron-based superconductors research
- Blood properties and coagulation
- Tuberculosis Research and Epidemiology
- Microbial bioremediation and biosurfactants
- Insect symbiosis and bacterial influences
- Metal Alloys Wear and Properties
- Muscle Physiology and Disorders
- Advanced Sensor and Energy Harvesting Materials
University of California, Los Angeles
2017-2025
Xi'an Jiaotong University
2022-2025
Hebei University of Technology
2023-2025
California NanoSystems Institute
2018-2024
Shanghai University
2023-2024
Southwest Jiaotong University
2020-2021
Shandong University
2012-2020
State Key Laboratory of Microbial Technology
2012-2020
UCLA Health
2019
Imaging Center
2017-2018
Abstract Plant diseases caused by fungi and oomycetes pose an increasing threat to food security ecosystem health worldwide. These filamentous pathogens, while taxonomically distinct, modulate host defense responses secreting effectors, which are typically identified based on the presence of signal peptides. Here we show that Phytophthora sojae Verticillium dahliae secrete isochorismatases (PsIsc1 VdIsc1, respectively) required for full pathogenesis. PsIsc1 VdIsc1 can suppress...
The spliceosome undergoes dramatic changes in a splicing cycle. Structures of B, B
We present an in-depth analysis of selected CASP15 targets, focusing on their biological and functional significance. The authors the structures identify discuss key protein features evaluate how effectively these aspects were captured in submitted predictions. While overall ability to predict three-dimensional continues impress, reproducing uncommon not previously observed experimental is still a challenge. Furthermore, instances with conformational flexibility large multimeric complexes...
Abstract Peripheral nerve regeneration and functional recovery rely on the chemical, physical, structural properties of guidance conduits (NGCs). However, limited support for long‐distance axonal has hindered widespread use NGCs. This study introduces a novel conduit with oriented lateral walls, incorporating multi‐walled carbon nanotubes (MWCNTs) within core‐shell fibers prepared in single step using modified electrohydrodynamic (EHD) printing technique to promote peripheral regeneration....
U1 snRNP plays a critical role in 5'-splice site recognition and is frequent target of alternative splicing factors. These factors transiently associate with human are not amenable for structural studies, while their Saccharomyces cerevisiae (yeast) homologs stable components snRNP. Here, we report the cryoEM structure yeast at 3.6 Å resolution atomic models ten core proteins, nearly all essential domains its RNA, five stably associated auxiliary proteins. The foot-shaped contains...
Human APOBEC3G (A3G) is a virus restriction factor that inhibits HIV-1 replication and triggers lethal hypermutation on viral reverse transcripts. infectivity (Vif) breaches this host A3G immunity by hijacking cellular E3 ubiquitin ligase complex to target for ubiquitination degradation. The molecular mechanism of targeting Vif-E3 unknown, limiting the antiviral efforts host-pathogen interaction crucial infection. Here, we report cryo-electron microscopy structures bound Vif in with T cell...
Abstract Following assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema (EF) into host cell. Here, we report cryo-EM structures of heptameric PA channels with partially unfolded LF EF at 4.6 3.1-Å resolution, respectively. The first α helix β strand unfold dock a deep amphipathic cleft, called clamp, which resides interface two monomers. α-clamp-helix interactions exhibit structural plasticity when...
Abstract The α-keto acid dehydrogenase complex family catalyzes the essential oxidative decarboxylation of acids to yield acyl-CoA and NADH. Despite performing same overarching reaction, members have different component structures structural organization between each other across phylogenetic species. While native complexes from bacteria fungi became available recently, atomic structure their mammalian counterparts in states remain unknown. Here, we report cryo-electron microscopy endogenous...
Nipah virus is an emergent paramyxovirus that causes deadly encephalitis and respiratory infections in humans. Two glycoproteins coordinate the infection of host cells, attachment protein (G), which binds to cell surface receptors, a fusion (F) protein, carries out process virus-cell membrane fusion. The G ephrin B2/3 inducing conformational changes trigger F refolding. Using optical approach based on second harmonic generation, we show monomeric dimeric receptors activate distinct G....
Cyclic diguanosine monophosphate (c-di-GMP) is one of the most important bacterial second messengers that controls many cellular functions including lifestyle switch between plankton and biofilm. Surface attachment defective (SadC) a diguanylate cyclase (DGC) involved in biosynthesis c-di-GMP Pseudomonas aeruginosa, an opportunistic pathogen can cause diverse infections. Here we report crystal structure GGDEF domain from SadC critical role trans-membrane (TM) with regard to biofilm...
Abstract Transcription by RNA polymerase V (Pol V) in plants is required for RNA-directed DNA methylation, leading to transcriptional gene silencing. Global chromatin association of Pol requires components the DDR complex DRD1, DMS3 and RDM1, but assembly process this underlying mechanism recruitment remain unknown. Here we show that all co-localize with V, report cryoEM structures two complexes associated recruitment—DR (DMS3-RDM1) DDR′ (DMS3-RDM1-DRD1 peptide), at 3.6 Å 3.5 resolution,...
In Trypanosoma brucei, the editosome, composed of RNA-editing substrate-binding complex (RESC) and catalytic (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism information transfer from gRNA mRNA is unclear owing a lack high-resolution structures for these complexes. With cryo-electron microscopy functional studies, we have captured gRNA-stabilizing RESC-A gRNA-mRNA-binding RESC-B RESC-C particles....
Abstract Female mosquitoes inject saliva into vertebrate hosts during blood feeding. This process transmits mosquito-borne human pathogens that collectively cause ~1,000,000 deaths/year. Among the most abundant and conserved proteins secreted by female salivary glands is a high-molecular weight protein called gland surface 1 (SGS1) facilitates pathogen transmission, but its mechanism remains elusive. Here, we determine native structure of SGS1 cryoID approach, showing 3364 amino-acid has Tc...
The internal oxidation formed during the advanced high-strength steels hot-rolled coiling process can be inherited into cold-rolled sheet and impact final product quality. Herein, influence of Si on oxide scale phase transition behavior Fe-Mn-Si steel after are investigated. results indicate that depth increases with increase content is accompanied by severe intragranular oxidation. Moreover, interface between Mn-Si substrate provides a direct diffusion path, which accelerates rate In...