A5103908571- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Amyotrophic Lateral Sclerosis Research
- dental development and anomalies
- Dental Radiography and Imaging
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Neurogenetic and Muscular Disorders Research
- Dental Implant Techniques and Outcomes
- Cancer Treatment and Pharmacology
- Orthodontics and Dentofacial Orthopedics
- Periodontal Regeneration and Treatments
- Protist diversity and phylogeny
- Prion Diseases and Protein Misfolding
- Microbial Community Ecology and Physiology
- Oral and Maxillofacial Pathology
- Muscle Physiology and Disorders
- Advanced biosensing and bioanalysis techniques
- Carbohydrate Chemistry and Synthesis
- Head and Neck Cancer Studies
- Oral microbiology and periodontitis research
- Microtubule and mitosis dynamics
- Cholinesterase and Neurodegenerative Diseases
- Endodontics and Root Canal Treatments
- Fungal Biology and Applications
Fujian Medical University
2018-2024
Shandong University
2012-2024
First Affiliated Hospital of Fujian Medical University
2018-2024
Beijing Language and Culture University
2024
The First Hospital of Changsha
2024
Central South University
2024
State Key Laboratory of Microbial Technology
2004-2023
Peking University
2009-2023
Qingdao Binhai University
2023
Qilu Hospital of Shandong University
2021
Abstract Complex environmental conditions can significantly affect bacterial genome size by unknown mechanisms. The So0157-2 strain of Sorangium cellulosum is an alkaline-adaptive epothilone producer that grows across a wide pH range. Here, we show the this 14,782,125 base pairs, 1.75-megabases larger than largest from S. reported previously. total 11,599 coding sequences (CDSs) include massive duplications and horizontally transferred genes, regulated lots protein kinases, sigma factors...
One intriguing discovery in modern microbiology is the extensive presence of extracellular DNA (eDNA) within biofilms various bacterial species. Although several biological functions have been suggested for eDNA, including involvement biofilm formation, detailed mechanism eDNA integration into architecture still poorly understood. In formed by Myxococcus xanthus, a Gram-negative soil bacterium with complex morphogenesis and social behaviors, was found both extracted native matrices (ECM)....
Myxococcus fulvus HW-1 (ATCC BAA-855) is a halotolerant marine myxobacterium. This strain exhibits complex social behaviors in the presence of low concentrations seawater but adopts an asocial living pattern under oceanic conditions. The whole genome M. will enable us to further investigate details its evolution.
Abstract The virulence factor pyocyanin and the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP) play key roles in regulating biofilm formation multi-drug efflux pump expression Pseudomonas aeruginosa . However, crosstalk between these two signaling pathways remains unclear. Here we show that BrlR (PA4878), previously identified as a c-di-GMP responsive transcriptional regulator, acts also receptor for pyocyanin. Crystal structures of free c-di-GMP-bound reveal...
NVMEERKIK, a peptide derived from ovalbumin, exhibited remarkable immunomodulatory activity. This study investigated the effect of simulated gastrointestinal digestion on its structure and bioactivity. NVMEERKIK was found to be unstable against enzymes completely degraded into NVME, NVMEE, KIK, K, R. Among these, which constituted 90.90% digest, synthesized demonstrated superior immune-enhancing activity than NVMEERKIK. Specifically, NVME improved phagocytosis, NO production, TNF-α content...
Abstract Objective To explore the associations between serum creatinine and creatine kinase (CK) levels with survival in male female ALS patients. Methods A prospective cohort study was carried out including 346 patients repeated CK measurements. Kaplan Meier analysis multivariable Cox regression were used to perform analysis. Results There 218 128 patients, males had significantly higher baseline than females. After analysis, lower associated a short both (≤61 μ mol/L, HR: 1.629; 95%CI:...
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease, pathologically characterized by TDP-43 aggregates. Recent evidence has been indicated that phosphorylated (pTDP-43) present not only in motor neurons but also muscle tissues. However, it unclear whether testing pTDP-43 aggregation tissue would assist the diagnosis of ALS. We propose three key questions: (i) Is detectable routine biopsied muscles? (ii) Can detection discriminate between ALS and non-ALS patients?...
Myxococcus xanthus is a bacterium capable of complex social organization. Its characteristic (“S”)-motility mechanism mediated by type IV pili (TFP), linear actuator appendages that propel the along surface. TFP are known to bind secreted exopolysaccharides (EPS), but it unclear how M. manages use TFP-EPS technology common many bacteria achieve its unique coordinated multicellular movements. We examine S-motility, using high-resolution particle-tracking algorithms, and observe aperiodic...
An unprecedented 19-membered allenic macrolide archangiumide (1) was discovered from the myxobacterium Archangium violaceum SDU8 by integrating NMR-based metabolic profiling and genome mining. Its biosynthesis pathway proposed based on architectural analysis of encoding trans-AT PKS genes validated isotope labeling. The methodology combing 2D bioinformatics-aided structure prediction, as exemplified this study, is anticipated to improve discovery efficiency a broader range microbial "dark matter".
There is a continuous need for novel microbial natural products to fill the drying-up drug development pipeline. Herein, we report myxadazoles from Myxococcus sp. SDU36, family of chimeric small molecules that consist N-ribityl 5,6-dimethylbenzimidazole and linear fatty acid chain endowed with an isoxazole ring. The experiments genome sequencing, gene insertion mutation, isotope labelling, precursor feeding demonstrated was encoded by non-canonical PKS/NRPS cluster, whereas origin related...
Amyotrophic lateral sclerosis (ALS) is an incurable and fatal neurodegenerative disease; most ALS patients die within 3 to 5 years after symptom onset, usually as a consequence of respiratory failure. In the present study, we aim screen survival-related pulmonary function parameters, explore predictive value peak expiratory flow (PEF) in disease severity prognosis with ALS.The discovery cohort included 202 patients, demographic clinical characteristics eligible were collected tests performed...
Social motility (S motility), the coordinated movement of large cell groups on agar surfaces, Myxococcus xanthus requires type IV pili (TFP) and exopolysaccharides (EPS). Previous models proposed that this behavior, which only occurred within groups, cycles TFP extension retraction triggered by close interaction with EPS. However, curious observation M. can perform TFP-dependent at a single-cell level when placed onto polystyrene surfaces in highly viscous medium containing 1%...
Abstract Myxococcus xanthus performs coordinated social motility of cell groups through the extension and retraction type IV pili (TFP) on solid surfaces, which requires both TFP exopolysaccharides (EPS). By submerging cells in a liquid medium containing 1% methylcellulose, M. TFP-driven was induced isolated independently EPS. We measured analyzed movements using community tracking algorithms, combine single-cell resolution with statistics from large sample populations. Cells without...
Abstract C-di-GMP (3’,5’ -Cyclic diguanylic acid) is an important second messenger in bacteria that influences virulence, motility, biofilm formation, and cell division. The level of c-di-GMP cells controlled by diguanyl cyclases (DGCs) phosphodiesterases (PDEs). Here, we report the biochemical functions crystal structure potential diguanylase Dcsbis (PA2771, a diguanylate cyclase with self-blocked I-site) from Pseudomonas aeruginosa PAO1. full-length protein contains N-terminal GAF domain...
Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with wide range of survival times. We aimed to explore prognostic factors related short based on clinical features and plasma metabolic signatures using surface‐enhanced Raman spectroscopy (SERS). One hundred thirty‐eight sporadic ALS cases were enrolled serially, including 62 for the short‐duration group (≤3 years) 76 long‐duration (>3 years). Multivariate analysis showed that an older age onset (>60...
Abstract Objective Amyotrophic lateral sclerosis (ALS) is an adult‐onset fatal neurodegenerative disease which lacks identified biological markers. A label‐free plasma surface‐enhanced Raman spectroscopy (SERS) method was developed to explore a simple and noninvasive test for ALS. Methods ALS patients were enrolled serially samples collected at the time of diagnosis prior start treatment. SERS spectra recorded using Renishaw micro‐Raman system. Results To exclude interference by varying...