A5070754234- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Neutropenia and Cancer Infections
- Endoplasmic Reticulum Stress and Disease
- Mass Spectrometry Techniques and Applications
- Blood disorders and treatments
- Autophagy in Disease and Therapy
- Cancer Treatment and Pharmacology
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Trypanosoma species research and implications
- Cellular transport and secretion
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Genetic Neurodegenerative Diseases
- Immunotherapy and Immune Responses
- RNA regulation and disease
University of California, Irvine
2016-2025
Chongqing Medical University
2023-2025
Children's Hospital of Chongqing Medical University
2023-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2025
Jilin University
2004-2025
Jilin Province Science and Technology Department
2004-2025
Institute of Laboratory Animal Science
2008-2024
Shanxi University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2008-2024
Suzhou Municipal Hospital
2024
The E6AP ubiquitin-protein ligase (E3) mediates the human papillomavirus-induced degradation of p53 tumor suppressor in cervical cancer and is mutated Angelman syndrome, a neurological disorder. crystal structure catalytic hect domain reveals bilobal with broad cleft at junction two lobes. consists conserved residues whose mutation interferes ubiquitin-thioester bond formation site syndrome mutations. bound to UbcH7 ubiquitin-conjugating enzyme (E2) determinants E2-E3 specificity provides...
Knowledge of elaborate structures protein complexes is fundamental for understanding their functions and regulations. Although cross-linking coupled with mass spectrometry (MS) has been presented as a feasible strategy structural elucidation large multisubunit complexes, this method proven challenging because technical difficulties in unambiguous identification cross-linked peptides determination sites by MS analysis. In work, we developed novel using newly designed MS-cleavable...
Expansion of the polyglutamine repeat within protein Huntingtin (Htt) causes Huntington's disease, a neurodegenerative disease associated with aging and accumulation mutant Htt in diseased neurons. Understanding mechanisms that influence cellular degradation may target treatments designed to activate clearance pathways. We find is phosphorylated by inflammatory kinase IKK, enhancing its normal proteasome lysosome. Phosphorylation regulates additional post-translational modifications,...
Tandem affinity strategies reach exceptional protein purification grades and have considerably improved the outcome of mass spectrometry-based proteomic experiments. However, current tandem tags are incompatible with two-step under fully denaturing conditions. Such stringent conditions desirable for spectrometric analyses modifications as they result in maximal preservation posttranslational modifications. Here we describe histidine-biotin (HB) tag, a new tag The HB consists hexahistidine...
Abstract CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive engineering requires simultaneous of multiple genes at defined locations. Here, to expand the scope Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a MbCas12a, possess high activity rice. Among them, Mb2Cas12a stands out with efficiency tolerance low temperature. An engineered...
Significance Blueprints of in-cell protein interaction landscapes are essential for our understanding cellular structures and functions, which have been challenging to study at the systems level. Cross-linking–mass spectrometry (XL-MS) represents a high-throughput method global profiling networks can determine identity connectivity native PPIs simultaneously without cell engineering. While in vivo XL-MS experiments feasible, in-depth analyses remain difficult due technical limitations on...
Tolerance of anoxia in maize root tips is greatly improved when seedlings are pretreated with 2 to 4 h hypoxia. We describe the patterns protein synthesis during hypoxic acclimation and anoxia. quantified incorporation [(35)S]methionine into total 262 individual proteins under different oxygen tensions. Proteins synthesized most rapidly normoxic conditions continued account for acclimation, while production a very few was selectively enhanced. When acclimated were placed anoxia, depressed no...
The 26S proteasome is a multisubunit complex responsible for degradation of ubiquitinated substrates, which plays critical role in regulating various biological processes. To fully understand the function and regulation complex, an important step to elucidate its subunit composition posttranslational modifications. Toward this goal, new affinity purification strategy has been developed using derivative HB tag rapid isolation human subsequent proteomic analysis. achieved from stable 293 cell...
The proteasome plays a pivotal role in the cellular response to oxidative stress. Here, we used biochemical and mass spectrometric methods investigate structural changes 26S proteasomes from yeast mammalian cells exposed hydrogen peroxide (H₂O₂). Oxidative stress induced dissociation of 20S core particle 19S regulatory proteasome, which resulted loss activities accumulation ubiquitinated proteins. H₂O₂ triggered increased association proteasome-interacting protein Ecm29 with purified...
Ubiquitination regulates a host of cellular processes by labeling proteins for degradation, but also functioning as regulatory, nonproteolytic posttranslational modification. Proteome-wide strategies to monitor changes in ubiquitination profiles are important obtain insight into the various functions ubiquitination. Here we describe generation stable cell lines expressing tandem hexahistidine-biotin tag (HB-tag) fused ubiquitin two-step purification ubiquitinated proteome under fully...
Dynamically interacting proteins associate and dissociate with their binding partners at high on/off rates. Although identification is of great significance to proteomics research, lack an efficient strategy distinguish stable dynamic interactors has hampered the efforts toward this goal. In work, we developed a new method, MAP (mixing after purification)-SILAC (stable isotope labeling amino acids in cell culture), quantitatively investigate interactions protein complexes by mass...
Protein–protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving physical contacts between proteins as they occur in cells is critical uncovering molecular details underlying various cellular activities. To advance study PPIs living cells, we have developed a new vivo cross-linking mass spectrometry platform that couples novel membrane-permeable, enrichable, MS-cleavable cross-linker with multistage tandem spectrometry. This strategy permits...
A thorough analysis of the protein interaction partners yeast GTPase Gsp1p was carried out by a multidimensional chromatography strategy strong cation exchange fractionation peptides followed reverse phase LC-ESI-MSMS using QSTAR instrument. This dataset then analyzed latest developmental version Protein Prospector. The Prospector search results were also compared with from engine "Mascot" new comparison program within named "SearchCompare." this study demonstrate that high quality data...
Huntingtin (Htt) is a widely expressed protein that causes tissue-specific degeneration when mutated to contain an expanded polyglutamine (poly(Q)) domain. Although Htt large, 350 kDa, the appearance of amino-terminal fragments in extracts postmortem brain tissue from patients with Huntington disease (HD), and fact fragment, exon 1 (Httex1p), sufficient cause models HD, points importance region process. The first encodes 17 amino acids followed by poly(Q) repeat variable length culminating...
Nedd8 is a small ubiquitin-like protein that can be conjugated to substrate–proteins in process known as neddylation. Although neddylation plays critical regulatory role cell proliferation and development, the spectrum of substrates its interaction network remain poorly understood. To explore pathway at proteome level, we have affinity purified modified associated proteins from HEK293 cells stably expressing GST-Nedd8 employed LC−MS/MS for subsequent identification. A total 496 been...
The number of publications in the field chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and probe protein-protein interactions has increased during last years. As technique is now becoming routine vitro vivo applications proteomics structural biology there a pressing need define protocols as well data analysis reporting formats. Such consensus formats should become accepted be shown lead reproducible...