A5055158467- CRISPR and Genetic Engineering
- interferon and immune responses
- Planetary Science and Exploration
- Ocular Diseases and Behçet’s Syndrome
- Astro and Planetary Science
- Geological and Geochemical Analysis
- Atmospheric chemistry and aerosols
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Air Quality and Health Impacts
- Magnetic Properties of Alloys
- Air Quality Monitoring and Forecasting
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- High-pressure geophysics and materials
- Geology and Paleoclimatology Research
- Paleontology and Stratigraphy of Fossils
- Atmospheric and Environmental Gas Dynamics
- Fire effects on ecosystems
- Space Science and Extraterrestrial Life
- Climate Change and Health Impacts
- Meteorological Phenomena and Simulations
- Climate variability and models
- Radioactive element chemistry and processing
Institute of Geology and Geophysics
2018-2025
Chinese Academy of Sciences
2015-2025
Zhejiang Normal University
2024
Fudan University
2010-2024
University of Electronic Science and Technology of China
2015-2024
Southwest University
2024
Ningbo Institute of Industrial Technology
2015-2024
Ministry of Education of the People's Republic of China
2024
Ministry of Natural Resources
2022-2024
Chongqing Medical University
2018-2023
Polyploidization has provided much genetic variation for plant adaptive evolution, but the mechanisms by which molecular evolution of polyploid genomes establishes architecture underlying species differentiation are unclear. Brassica is an ideal model to increase knowledge evolution. Here we describe a draft genome sequence oleracea, comparing it with that its sister B. rapa reveal numerous chromosome rearrangements and asymmetrical gene loss in duplicated genomic blocks, amplification...
The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas molecular biosciences, including functional genomics, genetics, applied biomedical research, agricultural biotechnology. In plant systems, however, a number hurdles currently exist that limit this technology from reaching its full potential. For example,...
Abstract The Moon has a magmatic and thermal history that is distinct from of the terrestrial planets 1 . Radioisotope dating lunar samples suggests most basaltic magmatism ceased by around 2.9–2.8 billion years ago (Ga) 2,3 , although younger basalts between 3 Ga have been suggested crater-counting chronology, which large uncertainties owing to lack returned for calibration 4,5 Here we report precise lead–lead age 2,030 ± 4 million basalt clasts Chang’e-5 mission, 238 U/ 204 Pb ratio ( µ...
Targeting specificity has been a barrier to applying genome editing systems in functional genomics, precise medicine and plant breeding. In plants, only limited studies have used whole-genome sequencing (WGS) test off-target effects of Cas9. The cause numerous discovered mutations is still controversial. Furthermore, WGS-based analysis Cpf1 (Cas12a) not reported any higher organism date.
CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated temperature sensitive in plants, but a lack of comprehensive understanding on Cas12a sensitivity plant cells has hampered effective application nucleases editing. We compared AsCas12a, FnCas12a, and LbCas12a for their efficiencies non-homologous end joining (NHEJ) repair profiles at four different temperatures rice. found AsCas12a more to it...
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in plant development and stress responses. Loss-of-function analysis of miRNA genes has been traditionally challenging due to lack appropriate knockout tools. In this study, single (OsMIR408 OsMIR528) gene families (miR815a/b/c miR820a/b/c) rice were targeted by CRISPR-Cas9. We showed strand conformation polymorphism (SSCP) is a more reliable method than restriction fragment length (RFLP) for identifying CRISPR-Cas9...
Abstract CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive engineering requires simultaneous of multiple genes at defined locations. Here, to expand the scope Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a MbCas12a, possess high activity rice. Among them, Mb2Cas12a stands out with efficiency tolerance low temperature. An engineered...
The evolution of the lunar magnetic field can reveal Moon’s interior structure, thermal history, and surface environment. mid-to-late-stage is poorly constrained, thus, existence a long-lived dynamo remains controversial. Chang’e-5 mission returned heretofore youngest mare basalts from Oceanus Procellarum uniquely positioned at midlatitude. We recovered weak paleointensities ~2 to 4 microtesla basalt clasts 2 billion years ago, attesting longevity until least midstage. This paleomagnetic...
Summary CRISPR ‐Cas9 and Cas12a are two powerful genome editing systems. Expression of in plants is typically achieved with a mixed dual promoter system, which Cas protein expressed by Pol II guide RNA species‐specific III such as U6 or U3. To achieve coordinated expression compact vector packaging, it desirable to express both components under single promoter. Previously, we demonstrated first‐generation transcript unit ( STU )‐Cas9 ‐Cas9‐ RZ , based on hammerhead ribozyme for processing s...
Summary Cytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C‐to‐T in plants, we first compared seven cytidine deaminases BE3‐like configuration rice. We found A3A/Y130F‐CBE_V01 resulted highest efficiency both rice and Arabidopsis . Furthermore, demonstrated this A3A/Y130F deaminase could be used iSpyMacCas9‐mediated at A‐rich PAMs. showcase its applications, applied for multiplexed generate microRNA‐resistant mRNA transcripts as well...
Summary CRISPR‐Cas9 is an emerging genome editing tool for reverse genetics in plants. However, its application functional study of non‐coding RNAs plants still at infancy. Despite being a major class RNAs, the biological roles circle (circRNAs) remain largely unknown Previous plant circRNA studies have focused on identification and annotation putative circRNAs, with their functions uninvestigated by genetic approaches. Here, we applied multiplexed strategy to efficiently acquire individual...
Abstract Micrometeorite impacts and solar wind irradiation, the dominant space weathering (SW) processes, largely modified compositions microtexture of soil materials on Moon. Here, we report SW characteristics Chang'e‐5 lunar soils from mid‐high latitude (43.06°N). All mineral phases exposed surface a single basalt clast have vapor deposit layer, whereas textures irradiation‐damaged zone are dependent host species. Nanophase Fe (npFe 0 ) particles spherical in amorphized pyroxenes,...
Summary PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine are promising tools for precise genome editing in plants. However, their genome‐wide off‐target effects largely unexplored. Here, we conduct whole‐genome sequencing (WGS) analyses of transgenic plants edited by xCas9, Cas9‐NGv1, Cas9‐NG, SpRY, nCas9‐NG‐PmCDA1, nSpRY‐PmCDA1 nSpRY‐ABE8e rice. Our results reveal that nuclease editors, when coupled with the same guide RNA (gRNA), prefer distinct gRNA‐dependent sites. De novo...
Abstract Cytokinin (CTK) is an important plant hormone that promotes cell division, controls differentiation, and regulates a variety of growth development processes. oxidase/dehydrogenase (CKX) irreversible cytokinin‐degrading enzyme affects by regulating the dynamic balance CTKs synthesis degradation. There are presumed 11 members CKX gene family in rice ( Oryza sativa L.), but limited have been reported. In this study, based on CRISPR‐Cas9 CRISPR‐Cas12a genome‐editing technology, we...