A5012743687- CRISPR and Genetic Engineering
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Heat shock proteins research
- Genetic Neurodegenerative Diseases
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Genetics, Aging, and Longevity in Model Organisms
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Animal Genetics and Reproduction
- T-cell and B-cell Immunology
- Prion Diseases and Protein Misfolding
- Metabolomics and Mass Spectrometry Studies
- Biopolymer Synthesis and Applications
- biodegradable polymer synthesis and properties
- Microscopic Colitis
- Alzheimer's disease research and treatments
University of California, Berkeley
2018-2024
QB3
2021-2024
Howard Hughes Medical Institute
2018
East Carolina University
2014-2017
Stanford University
2014-2016
Mayo Clinic in Florida
2013
McLean Hospital
1997
University of Virginia
1988
Duke University
1988
Duke University Hospital
1988
The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas molecular biosciences, including functional genomics, genetics, applied biomedical research, agricultural biotechnology. In plant systems, however, a number hurdles currently exist that limit this technology from reaching its full potential. For example,...
An expanded hexanucleotide repeat in C9orf72 causes amyotrophic lateral sclerosis and frontotemporal dementia (c9FTD/ALS). Therapeutics are being developed to target RNAs containing the sequence (GGGGCC); however, this approach is complicated by presence of antisense strand transcription GGCCCC repeats. We found that targeting elongation factor Spt4 selectively decreased production both sense transcripts, as well their translated dipeptide (DPR) products, also mitigated degeneration animal...
There are many studies suggesting an age-associated decline in the actin cytoskeleton, and this has been adopted as common knowledge field of aging biology. However, a direct identification phenomenon multicellular organisms not performed. Here, we express LifeAct::mRuby tissue-specific manner to interrogate cytoskeletal organization function age. We show for first time Caenorhabditis elegans that morphology cytoskeleton deteriorate at advanced age muscles, intestine, hypodermis. Moreover,...
The concentrations of selected metabolites in the hippocampus and cerebellum 13 Alzheimer's diseased (AD) four nondemented postmortem brains were measured using high resolution 1H NMR spectroscopy. For both hippocampal region cerebellum, putative neuronal marker N-acetyl aspartate (NAA) was significantly lower AD relative to brains. region, NAA concentration correlated inversely with semiquantitative assessments loss neurofibrillary tangles. γ-aminobutyric acid levels an age- a...
Abstract Oncogenic mutations can destabilize signaling proteins, resulting in increased or unregulated activity. Thus, there is considerable interest mapping the relationship between and stability of to better understand consequences oncogenic potentially inform development new therapeutics. Here, we develop a tool study protein-kinase live mammalian cells effects HSP90 chaperone system on these kinases. We monitor fluorescence kinases fused fluorescent protein relative that co-expressed...
Abstract Oncogenic mutations can destabilize signaling proteins, resulting in increased or unregulated activity. Thus, there is considerable interest mapping the relationship between and stability of to better understand consequences oncogenic potentially inform development new therapeutics. Here, we develop a tool study protein‐kinase live mammalian cells effects HSP90 chaperone system on these kinases. We determine expression levels protein kinases by monitoring fluorescence fluorescent...
ABSTRACT Longevity is dictated by a combination of environmental and genetic factors. One the key mechanisms implicated in regulating lifespan extension ability to induce protein chaperones promote homeostasis. However, it unclear whether exclusively regulate longevity. Previous work has shown that activating unfolded response endoplasmic reticulum (UPR ER ) neurons can signal peripheral tissues chaperone expression, thus enhancing organismal stress resistance extending lifespan. Here, we...