A5015795152- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Genetic Neurodegenerative Diseases
- Hereditary Neurological Disorders
- Ubiquitin and proteasome pathways
- Parathyroid Disorders and Treatments
- RNA Research and Splicing
- Nerve injury and regeneration
- Retinal Development and Disorders
- Nicotinic Acetylcholine Receptors Study
- Biochemical Analysis and Sensing Techniques
- Fungal and yeast genetics research
- Genetic and Kidney Cyst Diseases
- Neural dynamics and brain function
- Medicinal Plants and Neuroprotection
- Ion channel regulation and function
- Genetic Syndromes and Imprinting
- Gaze Tracking and Assistive Technology
- Neuroinflammation and Neurodegeneration Mechanisms
University of Chile
2015-2024
Instituto de Neurociencia Biomédica
2011-2023
Universidad Bernardo O'Higgins
2020
Neuroscience Institute
2018
Tufts University
2010
University of Pennsylvania
2007
University College London
1998-2005
Medical Research Council
1998-2004
MRC Laboratory for Molecular Cell Biology
1999-2004
Centro de Estudios Científicos
2004
GABA(B) receptors are unique among G-protein-coupled (GPCRs) in their requirement for heterodimerization between two homologous subunits, GABA(B1) and GABA(B2), functional expression. Whereas is capable of binding receptor agonists antagonists, the role each subunit signaling unknown. Here we identified amino acid residues within second intracellular domain GABA(B2) that critical coupling heterodimers to downstream effector systems. Our results provide strong evidence a G-protein...
γ-Aminobutyric acid type B (GABA<sub>B</sub>) receptors mediate the transmission of slow and prolonged inhibitory signals in central nervous system. Two splice variants GABA<sub>B</sub> receptors, GABA<sub>B</sub>R1a GABA<sub>B</sub>R1b, were recently cloned from a mouse cortical cerebellar cDNA library. As predicted, these belong to G protein-coupled receptor superfamily. We have used epitope-tagged versions study cellular distribution proteins variety non-neuronal neuronal cell types. Here...
Dendritic arborization of neurons is regulated by brain-derived neurotrophic factor (BDNF) together with its receptor, TrkB. Endocytosis required for dendritic branching and regulates TrkB signaling, but how postendocytic trafficking determines the neuronal response to BDNF not well understood. The monomeric GTPase Rab11 dynamics recycling endosomes local delivery receptors specific compartments. We investigated whether Rab11-dependent in dendrites BDNF-induced rat hippocampal neurons....
A major goal of neuroscience is understanding how neurons arrange themselves into neural networks that result in behavior. Most theoretical and experimental efforts have focused on a top-down approach which seeks to identify neuronal correlates behaviors. This has been accomplished by effectively mapping specific behaviors distinct patterns, or creating computational models produce desired behavioral outcome. Nonetheless, these approaches only implicitly considered the fact tissue, like any...
GABA B receptors are G-protein-coupled that mediate slow synaptic inhibition in the brain and spinal cord. These heterodimers assembled from B1 B2 subunits, neither of which is capable producing functional on homomeric expression. B1, although able to bind GABA, retained within endoplasmic reticulum (ER) when expressed alone. In contrast, access cell surface alone but does not couple efficiently appropriate effector systems or produce any detectable GABA-binding sites. present study, we have...
GABA<sub>A</sub> receptors (GABA<sub>A</sub>-Rs) play a significant role in mediating fast synaptic inhibition and it is the main inhibitory receptor CNS. The of <i>Wnt</i> signaling coordinating synapse structure function mature CNS poorly understood. In previous studies we found that ligands can modulate excitatory synapses through remodeling both presynaptic postsynaptic regions. this current study provide evidence for effect <i>Wnt-5a</i> on GABA<sub>A</sub>-Rs. We observed induces...
Slow and persistent synaptic inhibition is mediated by metabotropic GABA B receptors (GABA Rs). Rs are responsible for the modulation of neurotransmitter release from presynaptic terminals hyperpolarization at postsynaptic sites. Postsynaptic predominantly found on dendritic spines, adjacent to excitatory synapses, but control their plasma membrane availability still controversial. Here, we explore role glutamate receptor activation in regulating function surface central neurons. We...
In the adult hippocampus new neurons are continuously generated from neural stem cells (NSCs) present at subgranular zone of dentate gyrus. This process is controlled by Wnt signaling, which plays a complex role in regulating multiple steps neurogenesis including maintenance, proliferation and differentiation progenitor development newborn neurons. Differential effects signaling during progression could be mediated cell-type specific expression receptors. Here we studied potential Frizzled-1...
GABAB receptors are heterodimeric G protein-coupled that mediate slow synaptic inhibition in the central nervous system. The dynamic control of cell surface stability is likely to be fundamental importance modulation receptor signaling. Presently, however, this process poorly understood. Here we demonstrate remarkably stable at plasma membrane showing little basal endocytosis cultured cortical and hippocampal neurons. In addition, show exposure baclofen, a well characterized agonist, fails...
Neuronal GABA(B) receptors regulate calcium and potassium currents via G-protein-coupled mechanisms play a critical role in long-term inhibition of synaptic transmission the CNS. Recent studies have demonstrated that assembly receptor GABA(B)R1 GABA(B)R2 subunits into functional heterodimers is required for coupling to channels heterologous systems. However whether heterodimerization effector systems neurons remains be established. To address this issue, we studied recombinant endogenous...
Fast synaptic inhibition in the brain is largely mediated by gamma-aminobutyric acid receptors (GABA(A)R). While pharmacological manipulation of GABA(A)R function therapeutic agents, such as benzodiazepines can have profound effects on neuronal excitation and behavior, endogenous mechanisms neurons use to regulate efficacy their impact behavior remains poorly understood. To address this issue, we created a knock-in mouse which tyrosine phosphorylation GABA(A)Rs gamma2 subunit,...
Significance Regulation of the axonal protein content is fundamental to maintain neural function. The transport ready-made proteins by fast and slow mechanisms has been extensively studied, but local synthesis may also define composition axon, in particular during adaptation or recovery. Accumulated evidence suggests that a proportion cytosolic are locally translated, very little known about membrane proteins. Our findings show distribution endoplasmic reticulum secretory organelles...
The efficacy of synaptic transmission depends on the availability ionotropic and metabotropic neurotransmitter receptors at plasma membrane, but contribution endocytic recycling pathways in regulation gamma-aminobutyric acid type B (GABA(B)) remains controversial. To understand mechanisms that regulate abundance GABA(B) receptors, we have studied their turnover combining surface biotin labeling a microscopic immunoendocytosis assay hippocampal cortical neurons. We report internalization is...
The quantification of colocalizing signals in multichannel fluorescence microscopy images depends on the reliable segmentation corresponding regions interest, selection appropriate colocalization coefficients, and a robust statistical criterion to discriminate true from random colocalization. Here, we introduce confined displacement algorithm based image correlation spectroscopy combination with Manders coefficients M1(ROI) M2(ROI) quantify given florescence pattern. We show that existing...
GABA(B) receptors are heterodimeric G protein-coupled that mediate slow synaptic inhibition in the central nervous system. Whereas heterodimerization between receptor GABA(B)R1 and GABA(B)R2 subunits is essential for functional expression, how neurons coordinate assembly of these critical remains to be established. Here we have identified Marlin-1, a novel receptor-binding protein associates specifically with subunit yeast, tissue culture cells, neurons. Marlin-1 expressed brain exhibits...