A5025830796- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Biochemical effects in animals
- GABA and Rice Research
- Effects and risks of endocrine disrupting chemicals
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Gene Regulatory Network Analysis
- Trace Elements in Health
- Epilepsy research and treatment
- Stress Responses and Cortisol
- Estrogen and related hormone effects
- Molecular Sensors and Ion Detection
- Ion channel regulation and function
- Pancreatic function and diabetes
- Computational Drug Discovery Methods
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Analytical Chemistry and Chromatography
- Metabolism, Diabetes, and Cancer
University College London
2002-2016
University of London
2000-2002
The assessment of mixture effects estrogenic agents is regarded as an issue high priority by many governmental agencies and expert decision-making bodies all over the world. However, few studies published so far have suffered from conceptual experimental problems are considered to be inconclusive. Here, we report results assessments two-, three- four-component mixtures o,p'-DDT, genistein, 4-nonylphenol, 4-n-octylphenol, compounds with well-documented activity. Extensive...
Slow and persistent synaptic inhibition is mediated by metabotropic GABA B receptors (GABA Rs). Rs are responsible for the modulation of neurotransmitter release from presynaptic terminals hyperpolarization at postsynaptic sites. Postsynaptic predominantly found on dendritic spines, adjacent to excitatory synapses, but control their plasma membrane availability still controversial. Here, we explore role glutamate receptor activation in regulating function surface central neurons. We...
GABAB receptors mediate slow synaptic inhibition in the central nervous system and are important for plasticity as well being implicated disease. Located at pre- postsynaptic sites, will influence cell excitability, but their effectiveness doing so be dependent, part, on trafficking to, stability on, surface membrane. To examine dynamic behavior of GIRK cells neurons, we have devised a method that is based tagging receptor with binding site components neurotoxin, α-bungarotoxin. By using...
GABA B receptors mediate slow inhibitory neurotransmission in the brain and feature during excitatory synaptic plasticity, as well various neurological conditions. These are obligate heterodimers composed of R1 R2 subunits. The two predominant isoforms differ by presence complement control protein modules or Sushi domains (SDs) N terminus R1a. By using live imaging, with an α-bungarotoxin-binding site (BBS) fluorophore-linked bungarotoxin, we studied how stabilizes R1b subunits at cell...
GABA type A (GABA<sub>A</sub>) receptors are functionally regulated by external protons in a manner dependent on the receptor subunit composition. Although H<sup>+</sup> can regulate open probability of single ion channels, exactly what residues and subunits responsible for proton-induced modulation remain unknown. This study resolves this issue using recombinant α1βi GABA<sub>A</sub> expressed human embryonic kidney cells. The potentiating effect low pH responses exhibited p<sub>Ka</sub>...
Regulation of GABA(A) receptors by extracellular pH exhibits a dependence on the receptor subunit composition. To date, molecular mechanism responsible for modulation at alkaline has remained elusive. We report here that GABA-activated current can be potentiated 8.4 both alphabeta and gamma subunit-containing receptors, but only GABA concentrations below EC40. Site-specific mutagenesis revealed single lysine residue, K279 in beta TM2-TM3 linker, was critically important to modulate function...
Hyperekplexia or startle disease is a serious neurological condition affecting newborn children and usually involves dysfunctional glycinergic neurotransmission. Glycine receptors (GlyRs) are major mediators of inhibition in the spinal cord brainstem. A missense mutation, replacing asparagine (N) with lysine (K), at position 46 GlyR α1 subunit induced hyperekplexia following reduction potency transmitter glycine; this resulted from rapid deactivation agonist current mutant GlyRs. These...
The assessment of mixture effects estrogenic agents is regarded as an issue high priority by many governmental agencies and expert decision-making bodies all over the world. However, few studies published so far have suffered from conceptual experimental problems are considered to be inconclusive. Here, we report results assessments two-, three- four-component mixtures o,p'-DDT, genistein, 4-nonylphenol, 4-n-octylphenol, compounds with well-documented activity. Extensive...