A5060521527- Protein Tyrosine Phosphatases
- Protein Kinase Regulation and GTPase Signaling
- HER2/EGFR in Cancer Research
- Advanced Breast Cancer Therapies
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- 14-3-3 protein interactions
- Fibroblast Growth Factor Research
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- Angiogenesis and VEGF in Cancer
- Prostate Cancer Treatment and Research
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Synthesis and biological activity
- Biological Research and Disease Studies
- Heat shock proteins research
- Cellular Mechanics and Interactions
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
Australian Regenerative Medicine Institute
2016-2025
Monash University
2016-2025
Rotunda Hospital
2024
Royal College of Surgeons in Ireland
2024
Johns Hopkins University Applied Physics Laboratory
2023
Discovery Institute
2016-2022
John Wiley & Sons (United States)
2018
Hudson Institute
2018
Garvan Institute of Medical Research
2006-2015
The Kinghorn Cancer Centre
2012-2015
A cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound 2 or GRB2) containing one src homology (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal receptors (EGFRs) platelet-derived (PDGFRs) via its SH2 domain. Interestingly, exhibits striking structural functional to the C. elegans sem-5. It has been shown sem-5 other genes called let-23 (EGFR like) let-60 (ras lie...
Abstract Summary Structural and physiochemical descriptors extracted from sequence data have been widely used to represent sequences predict structural, functional, expression interaction profiles of proteins peptides as well DNAs/RNAs. Here, we present iFeature, a versatile Python-based toolkit for generating various numerical feature representation schemes both protein peptide sequences. iFeature is capable calculating extracting comprehensive spectrum 18 major encoding that encompass 53...
Abstract With the explosive growth of biological sequences generated in post-genomic era, one most challenging problems bioinformatics and computational biology is to computationally characterize sequences, structures functions an efficient, accurate high-throughput manner. A number online web servers stand-alone tools have been developed address this date; however, all these their limitations drawbacks terms effectiveness, user-friendliness capacity. Here, we present iLearn, a comprehensive...
Breast cancer is a common malignancy with current biological therapies tailored to steroid hormone (ER, PR) and HER2 receptor status. Understanding the basis of resistance targeted identification new potential therapeutic targets an ongoing challenge. The PI3K pathway altered in high proportion breast cancers may contribute resistance. We undertook integrative study mutational, copy number expression analyses key regulators cohort 292 invasive patients known treatment outcomes. alterations...
Protein kinase-like domains that lack conserved residues known to catalyse phosphoryl transfer, termed pseudokinases, have emerged as important signalling across all kingdoms of life. Although predicted function principally catalysis-independent protein-interaction modules, several pseudokinase been attributed unexpected catalytic functions, often amid controversy. We established a thermal-shift assay benchmark technique define the nucleotide-binding properties domains. Unlike in vitro...
Abstract Sequence-based analysis and prediction are fundamental bioinformatic tasks that facilitate understanding of the sequence(-structure)-function paradigm for DNAs, RNAs proteins. Rapid accumulation sequences requires equally pervasive development new predictive models, which depends on availability effective tools support these efforts. We introduce iLearnPlus, first machine-learning platform with graphical- web-based interfaces construction pipelines predictions using nucleic acid...
The Tek/Tie2 receptor tyrosine kinase plays a pivotal role in vascular and hematopoietic development. To study the signal transduction pathways that are mediated by this receptor, we have used yeast two-hybrid system to identify signaling molecules associate with phosphorylated Tek receptor. Using approach, demonstrate five molecules, Grb2, Grb7, Grb14, Shp2, p85 subunit of phosphatidylinositol 3-kinase can interact phosphotyrosine-dependent manner through their SH2 domains. Mapping binding...
The ubiquitin ligase Nedd4 has been proposed to regulate a number of signaling pathways, but its physiological role in mammals not characterized. Here we present an analysis Nedd4-null mice show that loss results reduced insulin-like growth factor 1 (IGF-1) and insulin signaling, delayed embryonic development, body weight, neonatal lethality. In mouse fibroblasts, mitogenic activity was reduced, the abundance adaptor protein Grb10 increased, IGF-1 receptor, which is normally on plasma...
Since their discovery a little more than decade ago, the docking proteins of Gab/DOS family have emerged as important signalling elements in metazoans. integrate and amplify signals from wide variety sources including growth factor, cytokine antigen receptors well cell adhesion molecules. They also contribute to signal diversification by channelling information activated into pathways with distinct biological functions. Recent approaches protein biochemistry systems biology revealed that Gab...
To identify therapeutic targets and prognostic markers for basal breast cancers, cancer cell lines were subjected to mass spectrometry-based profiling of protein tyrosine phosphorylation events. This revealed that luminal cells exhibit distinct signatures depend on pathway activation as well expression. Basal are characterized by elevated Met, Lyn, EphA2, epidermal growth factor receptor (EGFR), FAK, Src family kinase (SFK) substrates such p130Cas. SFKs exert a prominent role in these cells,...
Objective— Myocardial infarction (MI) is a serious complication of atherosclerosis associated with increasing mortality attributable to heart failure. Activation phosphoinositide 3-kinase [PI3K(p110α)] considered new strategy for the treatment However, whether PI3K(p110α) provides protection in setting MI unknown, and difficult target because it has multiple actions numerous cell types. The goal this study was assess beneficial and, if so, identify cardiac-selective microRNA mRNA that...