A5035637782- SARS-CoV-2 and COVID-19 Research
- HER2/EGFR in Cancer Research
- Semiconductor materials and devices
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Electrical and Thermal Properties of Materials
- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- SARS-CoV-2 detection and testing
- PI3K/AKT/mTOR signaling in cancer
- Electronic and Structural Properties of Oxides
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Renal Diseases and Glomerulopathies
- Estrogen and related hormone effects
- Copper Interconnects and Reliability
- Neonatal Health and Biochemistry
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Metal and Thin Film Mechanics
- Ferroelectric and Piezoelectric Materials
- Receptor Mechanisms and Signaling
- Advancements in Semiconductor Devices and Circuit Design
- Genetics, Aging, and Longevity in Model Organisms
- Muscle Physiology and Disorders
McMaster University
1970-2024
Lunenfeld-Tanenbaum Research Institute
2007-2024
Mount Sinai Hospital
2006-2024
Hamilton Health Sciences
2023
Sinai Health System
2022
University of Massachusetts Chan Medical School
2010-2017
McGill University
2004-2013
University of Toronto
2006-2013
Occupational Cancer Research Centre
2013
Goodman (Japan)
2013
This report concerns three individuals with a similar illness characterized by the following: striking leukocytosis due primarily to increased numbers of mature eosinophils; cardiac or pulmonary symptoms, both; and hepatosplenomegaly. Two patients died; third remains in clinical good health. Distinctive morphologic abnormalities were found heart, bone marrow, liver, spleen. Similar cases have often been reported as examples eosinophilic leukemia. However, review pertinent evidence is not...
The SARS-CoV-2 virus Omicron variant has now supplanted wild-type as the dominant circulating strain globally. Three doses of mRNA COVID-19 vaccine are recommended for transplant recipients their primary series. However, immunogenicity vaccines they specifically relate to not well studied. We analyzed Omicron-specific neutralization in after three-doses mRNA-1273 vaccine. Neutralization was determined using a spike pseudotyped lentivirus assay with constructs and Delta variants. A total 60...
The neu ( c-erbB-2 , Her-2 ) protooncogene is amplified and overexpressed in 20–30% of human breast cancers. Although transgenic mouse models have illustrated the role Neu induction mammary tumors, expression these driven by a strong viral promoter questionable relevance to disease. To ascertain whether activated under control endogenous gland could induce tumors we generated mice that conditionally express transcriptional intact promoter. Expression oncogenic resulted accelerated...
Amplification of the Neu/c-erbB-2 receptor tyrosine kinase has been implicated as an important event in genesis human breast cancer. Indeed, transgenic mice bearing either activated form neu or wild-type proto-oncogene under transcriptional control mouse mammary tumor virus promoter-enhancer frequently develop carcinomas (L. Bouchard, L. Lamarre, P. J. Tremblay, and Jolicoeur, Cell 57:931-936, 1989; C. T. Guy, M. A. Webster, Schaller, Parson, R. D. Cardiff, W. Muller, Proc. Natl. Acad. Sci....
BACKGROUNDLimited information is available on the impact of immunosuppressants COVID-19 vaccination in patients with immune-mediated inflammatory diseases (IMID).METHODSThis observational cohort study examined immunogenicity SARS-CoV-2 mRNA vaccines adult bowel disease, rheumatoid arthritis, ankylosing spondylitis, or psoriatic without maintenance immunosuppressive therapies. Ab and T cell responses to SARS-CoV-2, including neutralization against variants, were determined before after 1 2...
Safe and effective vaccines are needed to end the COVID-19 pandemic. Here, we report preclinical development of a lipid nanoparticle–formulated SARS-CoV-2 mRNA vaccine, PTX-COVID19-B. PTX-COVID19-B was chosen among three candidates after initial mouse vaccination results showed that it elicited strongest neutralizing antibody response against SARS-CoV-2. Further tests in mice hamsters indicated induced robust humoral cellular immune responses completely protected vaccinated animals from...
Amplification of the Neu/c-erbB-2 receptor tyrosine kinase has been implicated as an important event in genesis human breast cancer. Indeed, transgenic mice bearing either activated form neu or wild-type proto-oncogene under transcriptional control mouse mammary tumor virus promoter-enhancer frequently develop carcinomas (L. Bouchard, L. Lamarre, P. J. Tremblay, and Jolicoeur, Cell 57:931-936, 1989; C. T. Guy, M. A. Webster, Schaller, Parson, R. D. Cardiff, W. Muller, Proc. Natl. Acad. Sci....
AbstractSignaling mediated by ErbB2 is thought to play a critical role in numerous developmental processes. However, due the embryonic lethality associated with germ line inactivation of erbB2, its adult tissues remains largely obscure. Given expression at neuromuscular junction, we have created muscle-specific knockout assess there. This resulted viable mice progressive defect proprioception loss muscle spindles. Interestingly, partial reduction levels also reduced number Although...
Modular interaction domains that recognize peptide motifs in target proteins can impart selectivity signaling pathways. Phosphotyrosine binding (PTB) are components of cytoplasmic docking bind cell surface receptors through NPXY motifs. We have employed a library human proteome-derived NXXY sequences to explore PTB domain specificity and function. SPOTS arrays were used create comprehensive matrix receptor probed with set 10 diverse domains. This approach confirmed individual selective...
Abstract Prioritizing Ontario’s long-term care home (LTCH) residents for vaccination against severe acute respiratory syndrome coronavirus 2 has drastically reduced their disease burden; however, recent LTCH outbreaks of variants concern (VOCs) have raised questions regarding immune responses. In 198 residents, mRNA vaccine dose 1 elicited partial spike and receptor binding domain antibody responses, while the second a response at least equivalent to convalescent individuals in most...
Abstract Immunocompromised patients are predisposed to severe COVID-19. Here we compare homotypic and heterotypic humoral cellular immune responses Omicron BA.1 in organ transplant across a diverse clinical spectrum. We perform variant-specific pseudovirus neutralization assays for D614G, Omicron-BA.1, -BA.2, Delta variants. also measure poly-and monofunctional T-cell ancestral SARS-CoV-2 peptide pools. identify that partially or fully-vaccinated recipients after infection with have the...
Activation of the epidermal growth factor receptor (EGFR) family is thought to play a critical role in both embryogenesis and oncogenesis. The diverse biological activities EGFR are achieved through various ligand-receptor receptor-receptor interactions. One that has been found central this signaling network ErbB-2/Neu, it considered preferred heterodimerization partner for other members family. To assess importance catalytic activity ErbB-2 embryonic development, we have generated mice...
Changes in protein-protein interactions may allow polypeptides to perform unexpected regulatory functions. Mammalian ShcA docking proteins have amino-terminal phosphotyrosine (pTyr) binding (PTB) and carboxyl-terminal Src homology 2 (SH2) domains, which recognize specific pTyr sites on activated receptors, a central region with two phosphorylated tyrosine-X-asparagine (pYXN) motifs (where X represents any amino acid) that each bind the growth factor receptor–bound protein (Grb2) adaptor....
Shc family proteins serve as phosphotyrosine adaptor molecules in various receptor-mediated signaling pathways. In mammals, three distinct genes have been described that encode characterized by two phosphotyrosine-interaction modules, an amino-terminal binding (PTB) domain and a carboxy-terminal Src homology 2 domain. Here, we report the analysis of uncharacterized fourth protein, ShcD/Shc4, is expressed adult brain skeletal muscle. Consistent with this expression pattern, find ShcD can...
Abstract Background In solid organ transplant (SOT) recipients, the primary vaccination series against Coronavirus Disease 2019 is 3 doses followed by boosters. We determined whether a fourth dose booster induced Omicron BA.4/5 neutralizing antibodies (nAbs) and T cells in large multicenter cohort study. Methods Serum was collected 4–6 weeks post-third post-fourth of messenger RNA vaccine 222 SOT recipients. nAbs were measured using pseudovirus neutralization assay that targeted spike...
Abstract Background Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order analyze the large number of cDNAs available post-genomic era. In Creator system, an ORF introduced a donor vector can be transferred with Cre recombinase library acceptor optimized for different applications. Usability system is impacted by ability easily manipulate DNA, downstream applications, and level protein from vectors. Results To...
Nephrin is a key structural component of the podocyte slit diaphragm, and proper expression nephrin on cell surface critical to ensure integrity blood filtration barrier. Maintenance within this unique junction has been proposed require dynamic phosphorylation events endocytic recycling, although molecular mechanisms that control interplay are poorly understood. Here, we investigated possibility phosphotyrosine adaptor protein ShcA regulates turnover. Western blotting immunostaining analysis...
The Nucleosome Remodeling and Deacetylase (NuRD) complex is a chromatin regulatory that functions as transcriptional co-repressor in metazoans. NuRD subunit MBD3 essential for targeting assembly of functional well embryonic stem cell (ESC) pluripotency. Three isoforms (MBD3A, MBD3B, MBD3C) are expressed mouse. Here, we find the MBD3C isoform contains unique 50-amino-acid N-terminal region necessary to specifically interact with histone H3 binding protein WDR5. Domain analyses WDR5 reveal...