A5084136590- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Reactions
- Phosphodiesterase function and regulation
- Epigenetics and DNA Methylation
- Heat shock proteins research
- Asymmetric Hydrogenation and Catalysis
- Protein Structure and Dynamics
- Carbon dioxide utilization in catalysis
- Legume Nitrogen Fixing Symbiosis
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Mass Spectrometry Techniques and Applications
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Chemical Synthesis and Analysis
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Nicotinic Acetylcholine Receptors Study
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- DNA Repair Mechanisms
- Trypanosoma species research and implications
- Endoplasmic Reticulum Stress and Disease
University of New Hampshire
2015-2024
University of New Hampshire at Manchester
2014-2020
University of California, San Francisco
1999-2014
QB3
2014
Howard Hughes Medical Institute
2004-2007
University of California, Berkeley
2004
University of South Florida
1999-2002
Moffitt Cancer Center
1999
The unfolded protein response (UPR) adjusts the cell’s folding capacity in endoplasmic reticulum (ER) according to need. IRE1 is most conserved UPR sensor eukaryotic cells. It has remained controversial, however, whether mammalian and yeast use a common mechanism for ER stress sensing. Here, we show that similar yeast, human IRE1α’s ER-lumenal domain (hIRE1α LD) binds peptides with characteristic amino acid bias. Peptides proteins bind hIRE1α LD’s MHC-like groove induce allosteric changes...
Abstract Chromatophore organs in cephalopod skin are known to produce ultra-fast changes appearance for camouflage and communication. Light-scattering pigment granules within chromatocytes have been presumed be the sole source of coloration these complex organs. We report discovery structural emanating precise register with expanded pigmented chromatocytes. Concurrently, using an annotated squid chromatophore proteome together microscopy, we identify a likely biochemical component this...
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands redirection of scientific efforts criteria organized research projects. international COVID19-NMR consortium seeks provide such new approaches gathering expertise worldwide. In particular, making available viral proteins RNAs will pave way understanding molecular components in detail. resources provided through are fully disclosed accelerate access exploitation. NMR...
Inheritance of epigenetic information is critical for maintaining cell identity. The transfer parental histone H3-H4 tetramers, the primary carrier modifications on proteins, represents a crucial yet poorly understood step in inheritance information. Here, we show lagging strand DNA polymerase, Pol δ, interacts directly with and that interaction between δ sliding clamp PCNA regulates to strands, most likely independent their roles synthesis. When combined, mutations at Mcm2 compromise result...
Aurora B kinase is a key component of the chromosomal passenger complex (CPC), which regulates chromosome segregation and cytokinesis. An ortholog was characterized in Trypanosoma brucei (TbAUK1), but other conserved components have not been found. Here we identified four novel TbAUK1 associated proteins by tandem affinity purification mass spectrometry. Among these proteins, TbKIN-A TbKIN-B are kinesin homologs, whereas TbCPC1 TbCPC2 hypothetical without any sequence similarity to those...
Abstract The nuclear proteomes of maize (Zea mays) lines that differ in UV-B tolerance were compared by two-dimensional gel electrophoresis after UV light treatment. Differential accumulation chromatin proteins, particularly histones, constituted the largest class identified mass spectrometry. UV-B–tolerant landraces and B73 inbred line show twice as many protein changes UV-B–sensitive b, pl W23 transgenic expressing RNA interference constructs directed against factors. Mass spectrometic...
Chemical cross-linking, followed by identification of the cross-linked residues, is a powerful approach to probe topologies and interacting surfaces protein assemblies. In this work, we demonstrate new bioinformatics using multiple program modules within software package "Protein Prospector" that greatly facilitates discovery peptides in chemical cross-linking studies. Examples are given for how has been used defining interfaces heterodimeric homodimeric complexes, both which provide results...
In embryonic stem cells (ESCs), the Tip60 histone acetyltransferase activates genes required for proliferation and silences that promote differentiation. Here we show class II deacetylase Hdac6 co-purifies with Tip60-p400 complex from ESCs. is necessary regulation of most target genes, particularly those repressed by complex. Unlike differentiated cells, where mainly cytoplasmic, largely nuclear in ESCs, neural (NSCs), some cancer cell lines, interacts each. localizes to promoters bound...
Liquid chromatography/electrospray ionization mass spectrometry was used to investigate the peptide composition of venom Conus pennaceus, a molluscivorous cone shell from Red Sea. Based on observed Mrs, this contained all known conotoxins previously isolated and identified species. Interestingly, doubly protonated species only two these conotoxins, α-PnIA α-PnIB, showed additional related ions at +40 m/z (+80 Da), indicating presence either sulfation or phosphorylation in both components....
Among the methods used to unravel protein interaction surfaces, chemical cross-linking followed by identification of cross-linked peptides mass spectrometry has proven especially useful in dynamic and complex systems. During signal recognition particle (SRP)-dependent targeting proteins bacterial plasma membrane, specific between Ffh (the component SRP) FtsY SRP receptor) is known be essential for efficiency fidelity this process. In work, we studied Escherichia coli Thermus aquaticus...
Post-translational histone modifications modulate chromatin-templated processes and therefore affect cellular proliferation, growth, development. Although post-translational on the core histones have been under intense investigation for several years, variant are poorly understood. We used tandem mass spectrometry to identify covalent a H2A variant, macroH2A1.2. MacroH2A1.2 can be monoubiquitinated; however, site of monoubiquitination has not documented. In this study we green fluorescent...
Understanding the structure–function relationships of pigment-based nanostructures can provide insight into molecular mechanisms behind biological signaling, camouflage, or communication experienced in many species. In squid Doryteuthis pealeii, combinations phenoxazone-based pigments are identified as source visible color within nanostructured granules that populate dermal chromatophore organs. absence pigments, experience a reduction diameter with loss color, suggesting important...
Soil salinization is a worldwide problem that intensifying because of the effects climate change. An effective method for reclamation salt-affected soils involves initiating plant succession using fast growing, nitrogen fixing actinorhizal trees such as Casuarina. The salt tolerance Casuarina enhanced by nitrogen-fixing symbiosis they form with actinobacterium Frankia. Identification and molecular characterization salt-tolerant species associated Frankia imperative successful utilization in...