A5064631905- Iron Metabolism and Disorders
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Environmental Toxicology and Ecotoxicology
- CRISPR and Genetic Engineering
- Plant Micronutrient Interactions and Effects
- HIV/AIDS drug development and treatment
- Protein Degradation and Inhibitors
- Nanoparticles: synthesis and applications
- SARS-CoV-2 and COVID-19 Research
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- Heavy Metal Exposure and Toxicity
- Multiple Myeloma Research and Treatments
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Pharmaceutical and Antibiotic Environmental Impacts
- Molecular Biology Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Carcinogens and Genotoxicity Assessment
- HIV Research and Treatment
- Acute Myeloid Leukemia Research
- Radioactive element chemistry and processing
- Insect Resistance and Genetics
- RNA regulation and disease
University of Florida
2016-2025
Florida College
2017-2025
University Press of Florida
2024
UF Health Cancer Center
2022
University of Florida Health
2022
University of Mysore
2020
University of California, Berkeley
2010-2019
American Physiological Society
2019
Liverpool John Moores University
2015
University of Liverpool
2015
Most persons who are homozygous for C282Y, the HFE allele most commonly asssociated with hereditary hemochromatosis, have elevated levels of serum ferritin and transferrin saturation. Diseases related to iron overload develop in some C282Y homozygotes, but extent risk is controversial.We assessed mutations 31,192 northern European descent between ages 40 69 years participated Melbourne Collaborative Cohort Study were followed an average 12 years. In a random sample 1438 subjects stratified...
After reading many 2-DE-based articles featuring lists of the differentially expressed proteins, one starts experiencing a disturbing déjà vu. The same proteins seem to predominate regardless experiment, tissue or species. To quantify occurrence individual in 2-DE experiment reports, we compiled identities identified human, mouse, and rat tissues published three recent volumes Proteomics calculated appearance most predominant dataset. frequently protein is highly abundant glycolytic enzyme...
Toxicogenomics has provided innovative approaches to chemical screening, risk assessment, and predictive toxicology. If applied ecotoxicology, genomics tools could greatly enhance the ability understand modes of toxicity in environmentally relevant organisms. Daphnia magna, a small aquatic crustacean, is considered "keystone" species ecological food webs an indicator for toxicant exposure. Our objective was demonstrate potential utility gene expression profiling ecotoxicology by identifying...
Background: Differences in interlaboratory research protocols contribute to the conflicting data literature regarding engineered nanomaterial (ENM) bioactivity.Objectives: Grantees of a National Institute Health Sciences (NIEHS)-funded consortium program performed two phases vitro testing with selected ENMs an effort identify and minimize sources variability.Methods: Consortium participants (CPPs) conducted ENM bioactivity evaluations on zinc oxide (ZnO), three forms titanium dioxide (TiO2),...
Zinc oxide nanoparticles (ZnO NPs) are being rapidly developed for use in consumer products, wastewater treatment, and chemotherapy providing several possible routes ZnO NP exposure to humans aquatic organisms. Recent studies have shown that NPs undergo rapid dissolution Zn2+, but the relative contribution of Zn2+ bioavailability toxicity is not clear. We show a fraction suspension dissolves, this cannot account suspensions Daphnia magna. Gene expression profiling D. magna exposed or ZnSO4...
Iron deficiency is the most common nutritional disorder worldwide with substantial impact on health and economy. Current treatments predominantly rely soluble iron which adversely affects gastrointestinal tract. We have developed organic acid-modified Fe(III) oxo-hydroxide nanomaterials, here termed nano Fe(III), as alternative safe delivery agents. Nano absorption in humans correlated serum increase (P < 0.0001) direct vitro cellular uptake = 0.001), but not gastric solubility. The...
Nanowires (NWs), high-aspect-ratio nanomaterials, are increasingly used in technological materials and consumer products may have toxicological characteristics distinct from nanoparticles. We carried out a comprehensive evaluation of the physicochemical stability four silver nanowires (AgNWs) two sizes coatings their toxicity to Daphnia magna . Inorganic aluminum-doped silica were less effective than organic poly(vinyl pyrrolidone) at preventing oxidation or Ag(+) release underwent...
Hyalella azteca is a cryptic species complex of epibenthic amphipods interest to ecotoxicology and evolutionary biology. It the primary crustacean used in North America for sediment toxicity testing an emerging model molecular ecotoxicology. To provide resources quality assessments studies, we sequenced, assembled, annotated genome H. U.S. Lab Strain. The completeness comparable with other ecotoxicological species. Through targeted investigation use gene expression data sets exposed...
3' RNA sequencing provides an alternative to whole transcript analysis. However, we do not know a priori the relative advantage of each method. Thus, comprehensive comparison between and 3′ method is needed determine their merits. To this end, used two commercially available library preparation kits, KAPA Stranded mRNA-Seq kit (traditional method) Lexogen QuantSeq (3′ method), prepare libraries from mouse liver RNA. We then sequenced analyzed advantages disadvantages these approaches. found...
Abstract Background The COVID-19 pandemic has resulted in 275 million infections and 5.4 deaths as of December 2021. While effective vaccines are being administered globally, there is still a great need for antiviral therapies antigenically novel SARS-CoV-2 variants continue to emerge across the globe. Viruses require host factors at every step their life cycle, representing rich pool candidate targets drug design. Methods To identify that promote infection with potential broad-spectrum...
The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. aims of this study were to clone the rat gene and examine its expression gastrointestinal tract relation other genes encoding transport proteins. was isolated from mRNA found encode protein 96% identical mouse hephaestin. Analysis by ribonuclease protection assay Western blotting showed that expressed at high levels throughout small intestine colon. Immunofluorescence localized mature...