A5084352194- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Gut microbiota and health
- Child Nutrition and Water Access
- Trace Elements in Health
- Folate and B Vitamins Research
- Probiotics and Fermented Foods
- Heavy Metal Exposure and Toxicity
- Inflammatory Bowel Disease
- Nanoparticles: synthesis and applications
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Plant Micronutrient Interactions and Effects
- Food Security and Health in Diverse Populations
- Nutrition and Health in Aging
- Therapeutic Uses of Natural Elements
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Iron oxide chemistry and applications
- Diet, Metabolism, and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- COVID-19 Impact on Reproduction
- Erythropoietin and Anemia Treatment
- Insect Pheromone Research and Control
- Food composition and properties
University of Cambridge
2018-2024
MRC Unit the Gambia
2016-2023
Vifor Pharma (Switzerland)
2023
Vifor Pharma (United Kingdom)
2023
MRC Human Nutrition Research
2011-2021
Medical Research Council
2012-2018
University of Reading
2002-2003
Ghent University
1998-1999
ABSTRACT The objective of this study was to evaluate the effect human gut-derived lactic acid bacteria and bifidobacteria on cholesterol levels in vitro. Continuous cultures inoculated with fecal material from healthy volunteers media supplemented bile acids were used enrich for potential assimilators among indigenous bacterial populations. Seven probiotics found: Lactobacillus fermentum strains F53 KC5b, Bifidobacterium infantis ATCC 15697, Streptococcus bovis 43143, Enterococcus durans DSM...
ABSTRACT This study evaluated the use of a bile-salt-hydrolyzing Lactobacillus fermentum strain as probiotic with potential hypocholesterolemic properties. The effect L. on representative microbial populations and overall metabolic activity human intestinal microbiota was investigated using three-stage continuous culture system. Also, galactooligosaccharides prebiotic to enhance growth and/or evaluated. Administration resulted in decrease bifidobacterial population (ca. 1 log unit). In vitro...
Iron deficiency is the most common nutritional disorder worldwide with substantial impact on health and economy. Current treatments predominantly rely soluble iron which adversely affects gastrointestinal tract. We have developed organic acid-modified Fe(III) oxo-hydroxide nanomaterials, here termed nano Fe(III), as alternative safe delivery agents. Nano absorption in humans correlated serum increase (P < 0.0001) direct vitro cellular uptake = 0.001), but not gastric solubility. The...
Novel aspects of engineered nanoparticles offer many advantages for optimising food products and packaging. However, their potential hazards in the gastrointestinal tract require further investigation. We evaluated toxic inflammatory two types particles that might become increasingly relevant to industry, namely SiO2 ZnO. The materials were characterised morphology, oxidant generation hydrodynamic behaviour. Cytotoxicity interleukin-8 mRNA protein expression human intestinal Caco-2 cells....
The 2-5 nm Fe(III) oxo-hydroxide core of ferritin is less ordered and readily bioavailable compared to its pure synthetic analogue, ferrihydrite. We report the facile synthesis tartrate-modified, nano-disperse ferrihydrite small primary particle size, but with enlarged or strained lattice structure (~2.7Å for main Bragg peak versus 2.6Å ferrihydrite). Analysis indicated that co-precipitation conditions can be achieved tartrate inclusion into developing particles, retarding both growth...
Abstract Distinct bacterial trophic networks exist in the gut microbiota of individuals industrialized and non-industrialized countries. In particular, microbiomes tend to be enriched with Prevotella species. To study development these -rich compositions, we investigated children aged between 7 37 months living rural Gambia (616 children, 1,389 stool samples, stratified by 3-month age groups). These infants, who typically eat a high-fibre, low-protein diet, were part double-blind, randomized...
Dietary non-heme iron contains ferrous [Fe(II)] and ferric [Fe(III)] fractions the latter should hydrolyze, forming Fe(III) oxo-hydroxide particles, on passing from acidic stomach to less duodenum. Using conditions mimic in vivo hydrolytic environment we confirmed formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues these (~ 10 nm hydrodynamic diameter) were readily adherent cell membrane differentiated Caco-2 cells internalization was visualized using transmission...
The ferritin core is composed of fine nanoparticulate Fe3+ oxohydroxide, and we have developed a synthetic mimetic, polyoxohydroxide (nanoFe3+). aim this study was to determine how dietary iron derived in fashion absorbed the duodenum. Following 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented Fe2+ sulfate (FeSO4), nanoFe3+, no added Fe for further wk. A control group sufficient...
Abstract Alterations in the gut microbiota have been recently linked to oral iron. We conducted two feeding studies including an initial diet‐induced iron‐depletion period followed by supplementation with nanoparticulate tartrate‐modified ferrihydrite (Nano Fe(III): considered bioavailable host but not bacteria) or soluble ferrous sulfate (FeSO 4 : both and bacteria). applied denaturing gradient gel electrophoresis fluorescence situ hybridization for study‐1 454‐pyrosequencing of fecal 16S...
Oral iron supplementation is often associated with rapid onset of gastrointestinal side-effects. The aim this study was to develop and trial a short, simple questionnaire capture these early side-effects determine which symptoms are more discriminating. double-blind placebo-controlled randomized parallel one week treatment followed by wash-out. Subjects were into two groups (n = 10/group) receive either ferrous sulphate (200 mg capsules containing 65 iron) or placebo, both be taken at...
Iron (Fe) deficiency anemia remains the largest nutritional disorder worldwide. How gut acquires iron from nano Fe(III), especially at apical surface, is incompletely understood.We developed a novel Fe supplement consisting of nanoparticulate tartrate-modified Fe(III) poly oxo-hydroxide [here termed Fe(III)], which mimics oxide core ferritin and effectively treats in rats.We determined transfer to systemic circulation iron-deficient iron-sufficient outbread Swiss mouse strain (CD1) mice with...
Iron deficiency remains the largest nutritional worldwide and main cause of anaemia. Severe iron leads to anaemia known as (IDA), which affects a total 1·24 billion people, majority whom are children women from resource-poor countries. In sub-Saharan Africa, is frequently exacerbated by concomitant parasitic bacterial infections contributes over 120 000 maternal deaths year, while it irreparably limits cognitive development poor outcomes in pregnancy. Currently available compounds cheap...
Background: Iron deficiency and its associated anaemia (IDA) are the leading forms of micronutrient malnutrition worldwide. Here we describe rationale design first clinical trial evaluating efficacy safety an innovative nano iron supplement, hydroxide adipate tartrate (IHAT), for treatment IDA in young children (IHAT-GUT trial). Oral is often ineffective due to poor absorption and/or gastrointestinal adverse effects. IHAT novel since it effectively absorbed whilst remaining nanoparticulate...
Background Iron plays a key role in human immune responses; however, the influence of iron deficiency on coronavirus disease 2019 (COVID-19) vaccine effectiveness is unclear. Aim To assess BNT162b2 messenger RNA COVID-19 preventing severe acute respiratory syndrome 2 (SARS-CoV-2) infection and COVID-19–related hospitalization death individuals with or without deficiency. Methods This large retrospective, longitudinal cohort study analyzed real-world data from Maccabi Healthcare Services...
Iron deficiency anaemia and oral iron supplementation have been associated negatively with quality of life, adverse effects, respectively, in subjects inflammatory bowel disease (IBD). Hence, the risk-benefit ratio is not understood this patient group. The present case–control study investigated whether dietary intake impacts on life IBD patients. Quality habitual intakes requirements were assessed 29 patients inactive or mildly active as well 28 healthy control subjects. As expected, was...
Ferrous iron supplementation has been reported to adversely alter the gut microbiota in infants. To date, impact of on adult is limited, particularly at low supplementary concentrations. The aim this research was explore low-level healthy and Irritable Bowel Syndrome (IBS) volunteers. Anaerobic, pH-controlled vitro batch cultures were inoculated with faeces from or IBS donors along (ferrous sulphate, nanoparticulate pea ferritin (50 μmol−1 iron)). explored by fluorescence situ hybridisation...