A5041582063- Iron Metabolism and Disorders
- Diabetes and associated disorders
- Trace Elements in Health
- Pancreatic function and diabetes
- Diabetes Management and Research
- Plant Micronutrient Interactions and Effects
- Folate and B Vitamins Research
- Hemoglobinopathies and Related Disorders
- Heavy Metal Exposure and Toxicity
- Breastfeeding Practices and Influences
- Parathyroid Disorders and Treatments
- Fibroblast Growth Factor Research
- Child Nutrition and Water Access
- Biochemical Acid Research Studies
- Liver Diseases and Immunity
- Infant Nutrition and Health
- Digestive system and related health
- Collagen: Extraction and Characterization
- Microbial Applications in Construction Materials
- Blood Pressure and Hypertension Studies
- Cleft Lip and Palate Research
- Phytase and its Applications
- Sodium Intake and Health
- Alcoholism and Thiamine Deficiency
- Hydrogen's biological and therapeutic effects
University of Cambridge
2017-2024
MRC Human Nutrition Research
2004-2019
Medical Research Council
2013-2017
William Harvey Research Institute
2017
Queen Mary University of London
2017
Cambridge Research Biochemicals (United Kingdom)
2017
Wingate Institute
2004
University of California, Davis
2003
Iron deficiency is the most common nutritional disorder worldwide with substantial impact on health and economy. Current treatments predominantly rely soluble iron which adversely affects gastrointestinal tract. We have developed organic acid-modified Fe(III) oxo-hydroxide nanomaterials, here termed nano Fe(III), as alternative safe delivery agents. Nano absorption in humans correlated serum increase (P < 0.0001) direct vitro cellular uptake = 0.001), but not gastric solubility. The...
Exposure to persistent engineered nano and micro particles via the oral route is well established. Animal studies have demonstrated that, once ingested, a small proportion of such translocate from gastrointestinal tract other tissues. titanium dioxide widespread route, but only one study has provided indirect evidence (total analyses) absorption into blood stream in humans. We sought replicate these observations provide additional for particulate uptake.Human volunteers with normal...
The 2-5 nm Fe(III) oxo-hydroxide core of ferritin is less ordered and readily bioavailable compared to its pure synthetic analogue, ferrihydrite. We report the facile synthesis tartrate-modified, nano-disperse ferrihydrite small primary particle size, but with enlarged or strained lattice structure (~2.7Å for main Bragg peak versus 2.6Å ferrihydrite). Analysis indicated that co-precipitation conditions can be achieved tartrate inclusion into developing particles, retarding both growth...
High dietary sodium intake triggers increased blood pressure (BP). Animal studies show that salt loading results in dermal Na+ accumulation and lymphangiogenesis mediated by VEGF-C (vascular endothelial growth factor C), both attenuating the rise BP. Our objective was to determine whether these mechanisms function humans. We assessed skin electrolytes, BP, plasma 48 healthy participants randomized placebo (70 mmol sodium/d) slow (200 mmol/d) for 7 days. Skin K+ concentrations were measured...
Abstract Aims Heterogeneity in the rate of β ‐cell loss newly diagnosed type 1 diabetes patients is poorly understood and creates a barrier to designing interpreting disease‐modifying clinical trials. Integrative analyses baseline multi‐omics data obtained after diagnosis may provide mechanistic insight into diverse rates disease progression diagnosis. Methods We collected samples pan‐European consortium that enabled concerted analysis five different omics modalities from 97 patients. In...
Dietary non-heme iron contains ferrous [Fe(II)] and ferric [Fe(III)] fractions the latter should hydrolyze, forming Fe(III) oxo-hydroxide particles, on passing from acidic stomach to less duodenum. Using conditions mimic in vivo hydrolytic environment we confirmed formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues these (~ 10 nm hydrodynamic diameter) were readily adherent cell membrane differentiated Caco-2 cells internalization was visualized using transmission...
The ferritin core is composed of fine nanoparticulate Fe3+ oxohydroxide, and we have developed a synthetic mimetic, polyoxohydroxide (nanoFe3+). aim this study was to determine how dietary iron derived in fashion absorbed the duodenum. Following 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented Fe2+ sulfate (FeSO4), nanoFe3+, no added Fe for further wk. A control group sufficient...
Abstract Background The INNODIA consortium has established a pan-European infrastructure using validated centres to prospectively evaluate clinical data from individuals with newly diagnosed type 1 diabetes combined centralised collection of samples determine rates decline in beta-cell function and identify novel biomarkers, which could be used for future stratification phase 2 trials. Methods In this context, we have developed Master Protocol, based on the “backbone” natural history study,...
Myo-inositol hexakisphosphate (IP6), is the main iron chelator in cereals and bread. The aim of this study was to investigate effect three commercial baking processes (sourdough, conventional yeast Chorleywood Bread Making Process (CBP)) on IP6 content wholemeal bread, its impact uptake Caco-2 cells predicted bioavailability from these breads with added iron, simulating a mixed-meal. sourdough process fully degraded whilst CBP reduced it by 75% compared flour. released solution after...
Type 1 diabetes is a complex heterogenous autoimmune disease without therapeutic interventions available to prevent or reverse the disease. This study aimed identify transcriptional changes associated with progression in patients recent-onset type diabetes.Whole-blood samples were collected as part of INNODIA at baseline and 12 months after diagnosis diabetes. We used linear mixed-effects modelling on RNA-seq data genes age, sex, progression. Cell-type proportions estimated from using...
There is a growing need for markers that could help indicate the decline in beta cell function and recognise efficacy of intervention type 1 diabetes. Measurements suitably selected serum potentially provide non-invasive easily applicable solution to this challenge. Accordingly, we evaluated broad panel proteins previously associated with diabetes from newly diagnosed individuals during first year diagnosis. To uncover associations function, comparisons were made between these targeted...
Although the anti-Helicobacter pylori activity of bismuth is well established, therapeutic potential other metal ions against organism not known.We measured minimum inhibitory concentrations a series ions, including several cobalt (II) compounds four type strains and seven clinical isolates H. using three standard broth culture media defined medium. Other intestinal bacteria were also investigated for specificity action.Cobalt chloride had marked (minimum concentration range was 0.03-1.0...
Type 1 diabetes (T1D) is a chronic autoimmune disease, characterised by progressive destruction of the insulin-producing β cells pancreas. One immunosuppressive agent that has recently shown promise in treatment new-onset T1D subjects aged 12-45 years antithymocyte globulin (ATG), Thymoglobuline, encouraging further exploration lower age groups.Minimal effective low dose (MELD)-ATG phase 2, multicentre, randomised, double-blind, placebo-controlled, multiarm parallel-group trial participants...
In the UK contemporary estimates of dietary Fe intakes rely upon food content data from 1980s or before. Moreover, there has been speculation that natural foods fallen over time, predominantly due to changes in agricultural practices. Therefore, we re-analysed common plant-based diet for their (the ‘2000s analyses’) and compared values with most recent published ‘1980s much older ‘1930s analyses’), latter two being different editions McCance Widdowson tables. Overall, was remarkable...
Zinc delivery from a nipple shield system (NSDS), novel platform for administering medicines to infants during breastfeeding, was characterised using breastfeeding simulation apparatus. In this study, human milk at flow rates and pressures physiologically representative of passed through the NSDS loaded with zinc-containing rapidly disintegrating tablets, resulting in release zinc into milk. Inductively coupled plasma optical emission spectrometry used detect released, method that does not...
Fibroblast growth factor 23 (FGF23) is grossly elevated in Gambian children with rickets and, at a lower prevalence, those without bone deformities. We used western blotting to mimic the detection capabilities of C-terminal FGF23 enzyme-linked immunosorbent assay (ELISA). Only intact hormone was present plasma samples from and rickets.Elevated circulating concentrations have been detected using Immutopics ELISA. The ELISA detects both fragment. aim this study determine whether as by were...
Caco-2 cells may be typically used as a first step to investigate the bioavailability of different dietary and fortificant forms iron (Fe) at low levels (< 10 µM) in tissue culture medium (TCM). Whether this model is suitable with supplemental Fe (ca. 200 µM TCM) not clear neither, therefore, choice reference compound under those conditions ‘positive control’. Here we show that ?M TCM (serum-free MEM), Fe(II) sulphate precipitates while high ascorbic acid can prevent this, it detriment...