A5101874699- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Diabetes Management and Research
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Sphingolipid Metabolism and Signaling
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Prenatal Screening and Diagnostics
- IL-33, ST2, and ILC Pathways
- Protein Tyrosine Phosphatases
- Medical Imaging and Pathology Studies
- Single-cell and spatial transcriptomics
- Psoriasis: Treatment and Pathogenesis
- Genetic Syndromes and Imprinting
- Galectins and Cancer Biology
University of Turku
2021-2024
Åbo Akademi University
2021-2024
Turku Centre for Computer Science
2021-2024
Turku Centre for Biotechnology
2023
Type 1 diabetes is a complex heterogenous autoimmune disease without therapeutic interventions available to prevent or reverse the disease. This study aimed identify transcriptional changes associated with progression in patients recent-onset type diabetes.Whole-blood samples were collected as part of INNODIA at baseline and 12 months after diagnosis diabetes. We used linear mixed-effects modelling on RNA-seq data genes age, sex, progression. Cell-type proportions estimated from using...
Abstract Aims/hypothesis Type 1 diabetes is a chronic autoimmune disease of complex aetiology, including potential role for epigenetic regulation. Previous epigenomic studies focused mainly on clinically diagnosed individuals. The aim the study was to assess early DNA methylation changes associated with type already before diagnosis or even appearance autoantibodies. Methods Reduced representation bisulphite sequencing (RRBS) applied in purified CD4 + T cell, CD8 cell and − fractions 226...
Previous studies have revealed heterogeneity in the progression to clinical type 1 diabetes children who develop islet-specific antibodies either insulin (IAA) or glutamic acid decarboxylase (GADA) as first autoantibodies. Here, we test hypothesis that later disease different early immune responses, depending on of autoantibody appear (GADA-first IAA-first). We use mass cytometry for deep profiling peripheral blood mononuclear cell samples longitudinally collected from progressed (IAA-first,...
Gene regulatory elements, such as enhancers, greatly influence cell identity by tuning the transcriptional activity of specific types. Dynamics enhancer landscape during early human Th17 differentiation remains incompletely understood. Leveraging ATAC-seq-based profiling chromatin accessibility and comprehensive analysis key histone marks, we identified a repertoire enhancers that potentially exert control over fate specification cells. We found 23 SNPs associated with autoimmune diseases...
Regulatory T cells (Tregs) are central in controlling immune responses, and dysregulation of their function can lead to autoimmune disorders or cancer. Despite extensive studies on Tregs, the basis epigenetic regulation human Treg development is incompletely understood. Long intergenic noncoding RNAs (lincRNA)s important for shaping maintaining landscape different cell types. In this study, we identified a gene chromosome 6p25.3 locus, encoding lincRNA, that was up-regulated during early...
T cell activation, proliferation, and differentiation involve metabolic reprogramming resulting from the interplay of genes, proteins, metabolites. Here, we aim to understand pathways involved in activation functional human CD4+ subsets (T helper [Th]1, Th2, Th17, induced regulatory [iTreg] cells). combine genome-scale modeling, gene expression data, targeted non-targeted lipidomics experiments, together with vitro knockdown show that cells undergo specific changes during differentiation. In...
T helper 17 (Th17) cells protect against fungal and bacterial infections are implicated in autoimmunity. Several long intergenic noncoding RNAs (lincRNA) induced during Th17 differentiation, however, their contribution to differentiation is poorly understood. We aimed characterize the function of lincRNA Myocardial Infarction Associated Transcript (MIAT) early human cell differentiation. found MIAT be upregulated after induction along with an increase chromatin accessibility at gene locus....
DNA methylation patterns are largely established in-utero and might mediate the impacts of conditions on later health outcomes. Associations between perinatal marks pregnancy-related variables, such as maternal age gestational weight gain, have been earlier studied with microarrays, which typically cover less than 2% human CpG sites. To detect associations outside these regions, we chose bisulphite sequencing approach. We collected curated clinical data 200 newborn infants; whose umbilical...
Abstract Aims/hypothesis Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was determine whether perinatal is associated with later progression diabetes. Methods Reduced representation bisulphite sequencing (RRBS) analysis performed on umbilical cord samples within the Finnish Type Diabetes Prediction and Prevention (DIPP) Study. Children diagnosed and/or who tested positive for multiple islet...
Abstract Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides significant contribution the function regulatory T (Treg) cells. However, mechanism by which it regulates these processes was not apparent. To address this question, used affinity-purification mass...