A5003114342- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- interferon and immune responses
- ATP Synthase and ATPases Research
- Mycobacterium research and diagnosis
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Psoriasis: Treatment and Pathogenesis
- Protein Kinase Regulation and GTPase Signaling
- CAR-T cell therapy research
- Diamond and Carbon-based Materials Research
- Silk-based biomaterials and applications
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Bacteriophages and microbial interactions
- Sphingolipid Metabolism and Signaling
- Virus-based gene therapy research
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Mitochondrial Function and Pathology
Turku Centre for Computer Science
2019-2024
University of Turku
2017-2024
Åbo Akademi University
2017-2024
Turku Centre for Biotechnology
2017-2023
Cleveland Clinic Lerner College of Medicine
2023
In this paper, cellular management of fluorescent nanodiamonds (FNDs) has been studied for better understanding in the design potential applications FNDs biomedicine. The have shown to be photostable probes bioimaging and thus are well-suited, example, long-term tracking purposes. also exhibit good biocompatibility and, general, low toxicity cell labeling. To demonstrate underlying mechanism cells coping but potentially toxic effects by nondegradable FNDs, we their temporal intracellular...
Gene regulatory elements, such as enhancers, greatly influence cell identity by tuning the transcriptional activity of specific types. Dynamics enhancer landscape during early human Th17 differentiation remains incompletely understood. Leveraging ATAC-seq-based profiling chromatin accessibility and comprehensive analysis key histone marks, we identified a repertoire enhancers that potentially exert control over fate specification cells. We found 23 SNPs associated with autoimmune diseases...
Regulatory T cells (Tregs) are central in controlling immune responses, and dysregulation of their function can lead to autoimmune disorders or cancer. Despite extensive studies on Tregs, the basis epigenetic regulation human Treg development is incompletely understood. Long intergenic noncoding RNAs (lincRNA)s important for shaping maintaining landscape different cell types. In this study, we identified a gene chromosome 6p25.3 locus, encoding lincRNA, that was up-regulated during early...
Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) is an oncogene and a potential cancer therapy target protein. Accordingly, better understanding the physiological function CIP2A, especially in context immune cells, prerequisite for its exploitation therapy. Here, we report that CIP2A negatively regulates interleukin (IL)-17 production by Th17 cells human mouse. Interestingly, concomitant with increased IL-17 production, CIP2A-deficient had strength duration STAT3 phosphorylation. We...
T helper 17 (Th17) cells protect against fungal and bacterial infections are implicated in autoimmunity. Several long intergenic noncoding RNAs (lincRNA) induced during Th17 differentiation, however, their contribution to differentiation is poorly understood. We aimed characterize the function of lincRNA Myocardial Infarction Associated Transcript (MIAT) early human cell differentiation. found MIAT be upregulated after induction along with an increase chromatin accessibility at gene locus....
Sharpin, a multifunctional adaptor protein, regulates several signalling pathways. For example, Sharpin enhances signal-induced NF-κB as part of the linear ubiquitin assembly complex (LUBAC) and inhibits integrins, T cell receptor, caspase 1 PTEN. However, despite recent insights into LUBAC function, systematic approach to identify pathways regulated by has not been reported. Here, we present first 'Sharpin interactome', which identifies large number novel potential interactors in addition...
Cancerous inhibitor of protein phosphatase 2A (CIP2A) is involved in immune response, cancer progression, and Alzheimer's disease. However, an understanding the mechanistic basis its function this wide spectrum physiological pathological processes limited due to poorly characterized interaction networks. Here we present first systematic characterization CIP2A interactome by affinity-purification mass spectrometry combined with validation selected reaction monitoring targeted (SRM-MS)...
ABSTRACT SHARPIN is involved in several cellular processes and promotes cancer progression. However, how the choice between different functions of post-translationally regulated unclear. Here, we characterized phosphorylation by mass spectrometry vitro kinase assay. Focusing on S131 S146, demonstrate that they have a role SHARPIN-ARP2/3 complex interaction, but play no integrin inhibition or LUBAC activation. Consistent with its novel ARP2/3 regulation, S146 promoted lamellipodia formation....
Abstract Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides significant contribution the function regulatory T (Treg) cells. However, mechanism by which it regulates these processes was not apparent. To address this question, used affinity-purification mass...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Journal Cell Science, helping early-career researchers promote themselves alongside their papers. Meraj Hasan Khan author on ‘The Sharpin interactome reveals role for lamellipodium formation via Arp2/3 complex’, Science. PhD student laboratory Jeroen Pouwels at University Turku, Finland, investigating cytoskeleton and protein early markers metastasis.
ABSTRACT Cancerous inhibitor of protein phosphatase 2A (CIP2A) is involved in immune response, cancer progression, and Alzheimer’s disease. However, an understanding the mechanistic basis its function this wide spectrum physiological pathological processes limited due to poorly characterized interaction networks. Here we present first systematic characterization CIP2A interactome by affinity-purification mass spectrometry combined with validation selected reaction monitoring targeted...
Abstract The adaptor protein SHARPIN is involved in a number of cellular processes and promotes cancer progression metastasis. However, how the choice between different functions post-translationally regulated unclear. Here we have characterized phosphorylation by mass spectrometry vit ro kinase assay. Focusing on two uncharacterized sites, serine 131 146, unstructured linker region SHARPIN, demonstrate their role SHARPIN-ARP2/3 complex interaction, whereas they play no integrin inhibition...