A5019589476- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Cellular transport and secretion
- Sphingolipid Metabolism and Signaling
- Calcium signaling and nucleotide metabolism
- Viral Infections and Vectors
- Malaria Research and Control
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Circadian rhythm and melatonin
- Cancer-related molecular mechanisms research
- Mycobacterium research and diagnosis
- Adenosine and Purinergic Signaling
- Polyamine Metabolism and Applications
- Plant Parasitism and Resistance
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
Turku Centre for Computer Science
2019-2024
University of Turku
2018-2024
Åbo Akademi University
2018-2024
Turku Centre for Biotechnology
2018-2023
Keio University
2016-2017
International Centre for Genetic Engineering and Biotechnology
2016-2017
National Institute of Infectious Diseases
2015-2017
Regulatory T (Treg) cells are critical in regulating the immune response. In vitro induced Treg (iTreg) have significant potential clinical medicine. However, applying iTreg as therapeutics is complicated by poor stability of human and their variable suppressive activity. Therefore, it important to understand molecular mechanisms cell specification. We identified hypermethylated cancer 1 (HIC1) a transcription factor upregulated early during differentiation cells. Although FOXP3 expression...
Regulatory T cells (Tregs) are central in controlling immune responses, and dysregulation of their function can lead to autoimmune disorders or cancer. Despite extensive studies on Tregs, the basis epigenetic regulation human Treg development is incompletely understood. Long intergenic noncoding RNAs (lincRNA)s important for shaping maintaining landscape different cell types. In this study, we identified a gene chromosome 6p25.3 locus, encoding lincRNA, that was up-regulated during early...
T cell activation, proliferation, and differentiation involve metabolic reprogramming resulting from the interplay of genes, proteins, metabolites. Here, we aim to understand pathways involved in activation functional human CD4+ subsets (T helper [Th]1, Th2, Th17, induced regulatory [iTreg] cells). combine genome-scale modeling, gene expression data, targeted non-targeted lipidomics experiments, together with vitro knockdown show that cells undergo specific changes during differentiation. In...
Cytokinins are plant hormones that involved in regulation of cell proliferation, cycle progression, and plastid development. Here, we show the apicomplexan parasites Toxoplasma gondii Plasmodium berghei, an opportunistic human pathogen a rodent malaria agent, respectively, produce cytokinins via biosynthetic pathway similar to plants. regulate growth progression T. by mediating expression cyclin gene TgCYC4. A natural form cytokinin, trans-zeatin (t-zeatin), upregulated this cyclin, while...
Host cell microdomains are involved in the attachment, entry, and replication of intracellular microbial pathogens. Entry into host Toxoplasma gondii subsequent survival this protozoan parasite tightly coupled with proteins secreted from organelle called rhoptry. The rhoptry rapidly discharged clusters vesicles, evacuoles, which then delivered to parasitophorous vacuoles (PVs) or nucleus. In study, we examined roles two microdomain components, cholesterol glycosylphosphatidylinositol (GPI),...
The apicomplexan parasite Toxoplasma gondii produces the plant hormone abscisic acid, but it is unclear if phytohormones are produced by malaria Plasmodium spp., most important of this phylum. Here, we report detection salicylic an immune-related phytohormone land plants, in P. berghei ANKA and T. cell lysates. However, addition acid to falciparum culture had no effect. We transfected 3D7 with nahG gene, which encodes a acid-degrading enzyme isolated from plant-infecting Pseudomonas sp.,...
Abstract During phagocytic uptake by macrophages, role of Golgi apparatus was previously ruled out. Notably all such reports were limited to Fcγ-receptor mediated phagocytosis. Here we unravel a highly devolved mechanism for recruitment Golgi-derived secretory vesicles during phagosome biogenesis, which important most cargos except IgG-coated ones. We report Mannosidase-II positive diverse targets including latex beads, E. coli , Salmonella Typhimurium and Mycobacterium tuberculosis in human...
Abstract Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides significant contribution the function regulatory T (Treg) cells. However, mechanism by which it regulates these processes was not apparent. To address this question, used affinity-purification mass...
ABSTRACT T-cells are sentinels of adaptive cell-mediated immune responses. T-cell activation, proliferation and differentiation involves metabolic reprogramming involving the interplay genes, proteins metabolites. Here, we aim to understand pathways involved in activation functional human CD4 + subsets (Th1, Th2, Th17 iTregs). We combined genome-scale modeling, gene expression data, targeted non-targeted lipidomics experiments, together with vitro knockdown experiments showed that undergo...