A5029683473- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- Diabetes Management and Research
- Immune Response and Inflammation
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- Advanced Proteomics Techniques and Applications
- Rheumatoid Arthritis Research and Therapies
- Asthma and respiratory diseases
- Yersinia bacterium, plague, ectoparasites research
- Reproductive tract infections research
- Molecular Biology Techniques and Applications
- Lymphoma Diagnosis and Treatment
- interferon and immune responses
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
Åbo Akademi University
2016-2025
Turku Centre for Computer Science
2019-2025
University of Turku
2016-2025
Varsinais-Suomen Sairaanhoitopiiri
2021-2025
Turku Centre for Biotechnology
2012-2024
Novo Nordisk Foundation
2022
University of Copenhagen
2022
Aalto University
2014
Massachusetts Institute of Technology
2014
Oulu University Hospital
2014
Abstract Breast milk is a complex liquid with immune-competent cells and soluble proteins that provide immunity to the infant affect maturation of infant’s immune system. Exosomes are nanovesicles (30–100 nm) an endosome-derived limiting membrane secreted by diverse range cell types. Because exosomes carry immunorelevant structures, they suggested participate in directing response. We hypothesized human breast contain exosomes, which may be important for development isolated vesicles from...
The risk determinants of type 1 diabetes, initiators autoimmune response, mechanisms regulating progress toward β cell failure, and factors determining time presentation clinical diabetes are poorly understood. We investigated changes in the serum metabolome prospectively children who later progressed to diabetes. Serum metabolite profiles were compared between sample series drawn from 56 73 controls remained nondiabetic permanently autoantibody negative. Individuals developed had reduced...
Murine IL-10 has been reported originally to be produced by the Th2 subset of CD4+ T cell clones. In this study, we demonstrate that human is Th0, Th1-, and Th2-like clones after both Ag-specific polyclonal activation. purified peripheral blood cells, low, but significant, levels were found CD4+CD45RA+ population, whereas CD4+CD45RA- "memory" cells secreted 5- 20-fold higher IL-10. addition, was activated CD8+ cells. Optimal induction observed activation specific Ag combination anti-CD3 mAb...
T helper type 2 (Th2) cells are important regulators of mammalian adaptive immunity and have relevance for infection, autoimmunity, tumor immunology. Using a newly developed, genome-wide retroviral CRISPR knockout (KO) library, combined with RNA-seq, ATAC-seq, ChIP-seq, we dissected the regulatory circuitry governing activation differentiation these cells. Our experiments distinguish cell versus in quantitative framework. We demonstrate that two processes tightly coupled jointly controlled...
The insult leading to autoantibody development in children who will progress develop type 1 diabetes (T1D) has remained elusive. To investigate the genes and molecular pathways pathogenesis of this disease, we performed genome-wide transcriptomics analysis on a unique series prospective whole-blood RNA samples from at-risk collected Finnish Type Diabetes Prediction Prevention study. We studied 28 autoantibody-positive children, out which 22 progressed clinical disease. Collectively, covered...
Abstract Macrophages and dendritic cells (DC) are APC, which regulate innate adaptive immune responses. function locally mainly, maintaining inflammatory responses in tissues, whereas DC take up microbes, mature, migrate to local lymph nodes present microbial antigens naïve T elicit microbe-specific Blood monocytes can be differentiated vitro macrophages or by GM-CSF + IL-4, respectively. In the study, we performed global gene expression analyses using Affymetrix HG-U133A Gene Chip...
Chronic granulomatous disease (CGD) is a primary immunodeficiency caused by mutations in the phagocyte reactive oxygen species (ROS)-producing NOX2 enzyme complex and characterized recurrent infections associated with hyperinflammatory autoimmune manifestations. A translational, comparative analysis of CGD patients corresponding ROS-deficient Ncf1(m1J) mutated mouse model was performed to reveal molecular pathways operating deficient inflammation.A prominent type I interferon (IFN) response...
Although genome-wide association studies (GWAS) have identified hundreds of variants associated with a risk for autoimmune and immune-related disorders (AID), our understanding the disease mechanisms is still limited. In particular, more than 90% lie in non-coding regions, almost 10% these map to long RNA transcripts (lncRNAs). lncRNAs are known show cell-type specificity protein-coding genes. We aimed characterize genes located loci nine AIDs which been well-defined by Immunochip analysis...
Abstract Biomedical research typically involves longitudinal study designs where samples from individuals are measured repeatedly over time and the goal is to identify risk factors (covariates) that associated with an outcome value. General linear mixed effect models standard workhorse for statistical analysis of data. However, data can be complicated reasons such as difficulties in modelling correlated values, functional (time-varying) covariates, nonlinear non-stationary effects, model...
Identification of molecular components that define a pluripotent human embryonic stem cell (hESC) provides the basis for understanding mechanisms regulating maintenance pluripotency and induction differentiation. We compared gene expression profiles seven genetically independent hESC lines with those nonlineage‐differentiated cells derived from each line. A total 8,464 transcripts were expressed in all lines. More than 45% them have no yet‐known biological function, which indicates high...
Abstract Background Chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-seq) is increasingly being applied to study transcriptional regulation on a genome-wide scale. While numerous algorithms have recently been proposed for analysing the large ChIP-seq datasets, their relative merits and potential limitations remain unclear in practical applications. Results The present compares state-of-the-art detecting transcription factor binding sites four diverse datasets...