A5051862087- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Effects and risks of endocrine disrupting chemicals
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Toxic Organic Pollutants Impact
- Protein Kinase Regulation and GTPase Signaling
- Mechanisms of cancer metastasis
- Carcinogens and Genotoxicity Assessment
- Biochemical Acid Research Studies
- Ubiquitin and proteasome pathways
- S100 Proteins and Annexins
- 14-3-3 protein interactions
- Adipose Tissue and Metabolism
- Alzheimer's disease research and treatments
- Heavy Metal Exposure and Toxicity
- Sperm and Testicular Function
- Bladder and Urothelial Cancer Treatments
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Autophagy in Disease and Therapy
University of Chinese Academy of Sciences
2025
Kunming Institute of Botany
2025
Chinese Academy of Sciences
2025
Nanjing Drum Tower Hospital
2018-2024
Model Animal Research Center
2012-2024
Wuhan Pulmonary Hospital
2023-2024
Xiangya Hospital Central South University
2021-2024
University of Michigan
2023-2024
Michigan Medicine
2024
Central South University
2021-2024
Aducanumab, a human-derived antibody targeting amyloid-β (Aβ), is in Phase 3 clinical trials for the treatment of Alzheimer's disease. Biochemical and structural analyses show that aducanumab binds linear epitope formed by amino acids 3-7 Aβ peptide. Aducanumab discriminates between monomers oligomeric or fibrillar aggregates based on weak monovalent affinity, fast binding kinetics strong avidity epitope-rich aggregates. Direct comparative studies with analogs gantenerumab, bapineuzumab...
Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They characterized by the unique amino acids lanthionine and methyllanthionine, introduced means of dehydration Ser/Thr residues followed reaction resulting dehydro with cysteines form thioether linkages. Two-component lantibiotics use two each posttranslationally modified yield functionally distinct products act in synergy provide bactericidal activity. By...
Abstract Cadmium is one of the environmental and occupational pollutants its potential adverse effects on human health have given rise to substantial concern. causes damage male reproductive system via induction germ-cell apoptosis; however, underlying mechanism cadmium-induced toxicity in Leydig cells remains unclear. In this study, twenty mice were divided randomly into four groups exposed CdCl 2 at concentrations 0, 0.5, 1.0 2.0 mg/kg/day for consecutive weeks. Testicular injury, abnormal...
Abstract In primary biliary cirrhosis (PBC), the major autoepitope recognized by both T and B cells is inner lipoyl domain of E2 component pyruvate dehydrogenase. To address hypothesis that PBC induced xenobiotic exposure, we took advantage ab initio quantum chemistry synthesized dehydrogenase, replacing lipoic acid moiety with synthetic structures designed to mimic a xenobiotically modified hapten, quantitated reactivity these sera from patients. Interestingly, antimitochondrial Abs all...
The effect of pancreastatin on the release insulin, glucagon, and somatostatin was studied in isolated perfused rat pancreas. After an initial equilibration period (-20 to 0 min) with a basal glucose concentration (3.3 mM), pancreata were either 16.7 mM (0-40 or 20 arginine (0-20 min). Pancreastatin introduced 10 min prior throughout administration high continued during their perfusion. As expected, augmented insulin release. (1 nM) markedly suppressed first phase both insulinogogues used,...
Abstract The E2 subunit of pyruvate dehydrogenase complex (PDC-E2) is the major autoantigen recognized by antimitochondrial Abs (AMA) in primary biliary cirrhosis (PBC). Recently, we replaced lipoic acid moiety PDC-E2 with a battery synthetic structures designed to mimic xenobiotically modified lipoyl hapten on 12-aa peptide that was found within immunodominant autoepitope and demonstrated AMA PBC reacted against several organic mimotopes as well as, or sometimes significantly better than,...
AS160 (Akt substrate of 160 kDa) is a Rab GTPase-activating protein implicated in insulin control GLUT4 (glucose transporter 4) trafficking. In humans, truncation mutation (R363X) one allele decreased the expression and caused severe postprandial hyperinsulinaemia during puberty. To complement limited studies possible we generated an AS160-knockout mouse. wild-type mice, relatively high adipose tissue soleus muscle, low EDL (extensor digitorum longus) muscle detectable liver only after...
Deposition of tau aggregates in the brain is a pathological hallmark several neurodegenerative diseases, termed tauopathies, such as Alzheimer's disease (AD), corticobasal degeneration, and progressive supranuclear palsy (PSP). As transcellular spread has been implicated progression, immunotherapy being considered treatment for tauopathies. Here we report detailed biochemical biophysical characterization tau-binding properties gosuranemab, humanized monoclonal antibody directed against...
Significance Excess energy intake and physical inactivity are two major factors causing obesity, but the underlying mechanisms have not been fully understood. Both excess increase cellular status that is monitored by energy-sensing AMP-activated protein kinase (AMPK). We demonstrate AMPK–tre-2/USP6, BUB2, cdc16 domain family member 1 (the TBC GTPase activating domain) (TBC1D1) signaling nexus regulates insulin-like growth factor (IGF1) secretion. Disruption of this AMPK–TBC1D1 in mice...
Oxidative stress mechanisms are involved in hepatotoxicity. The liver is reported to be affected by bisphenol A (BPA) animals studies and has been also possess hepatic toxicity. This study aimed examine association between health status the effects of BPA on antioxidant defense systems biomarkers. (0, 2, 10, 50 mg/kg) body weight was mixed corn oil intraperitoneally administered every forty-eight hours for 30 days dose-dependent manner. There no significant difference rat BPA-treated groups...
SPEG (Striated muscle preferentially expressed protein kinase) has 2 kinase-domains and is critical for cardiac development function. However, it not clear how these function to maintain performance.To determine the molecular functions of SPEG.A proteomics approach identified SERCA2a (sarcoplasmic/endoplasmic reticulum calcium ATPase 2a) as a interacting with second kinase-domain but first SPEG. Furthermore, could phosphorylate Thr484 on SERCA2a, promote its oligomerization increase reuptake...
Abstract Diabetic cardiomyopathy is a progressive disease in diabetic patients, and myocardial insulin resistance contributes to its pathogenesis through incompletely-defined mechanisms. Striated muscle preferentially expressed protein kinase (SPEG) has two kinase-domains critical cardiac regulator. Here we show that SPEG phosphorylated on Ser 2461 /Ser 2462 /Thr 2463 by B (PKB) response insulin. PKB-mediated phosphorylation of activates second kinase-domain, which turn phosphorylates...
Abstract Bisphenol‐A (BPA), one of endocrine‐disrupting chemicals, is a male reproductive toxicant. Previous studies have revealed the direct cytotoxicity BPA in many cultured cells, such as mitotic aneuploidy embryonic cells and somatic apoptosis neurons testicular Sertoli cells. To understand action assess its risk, Pten/Akt pathway was investigated to elucidate mechanism effects BPA. The results showed that over 50 μM treatment could decrease viability cause more apoptosis. In addition,...